ID   LAH_ASPFU               Reviewed;        5538 AA.
AC   Q4X212;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Leashin {ECO:0000303|PubMed:23869404};
GN   Name=lah {ECO:0000303|PubMed:23869404}; ORFNames=AFUA_2G08060;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND SUBUNIT.
RX   PubMed=23869404; DOI=10.1111/mmi.12316;
RA   Beck J., Echtenacher B., Ebel F.;
RT   "Woronin bodies, their impact on stress resistance and virulence of the
RT   pathogenic mould Aspergillus fumigatus and their anchoring at the septal
RT   pore of filamentous Ascomycota.";
RL   Mol. Microbiol. 89:857-871(2013).
CC   -!- FUNCTION: Acts as the tether and is essential for anchoring of Woronin
CC       bodies at the septal pore (PubMed:23869404). In damaged hyphae, Woronin
CC       bodies occlude septal pores in order to separate intact from damaged
CC       compartments (PubMed:23869404). {ECO:0000269|PubMed:23869404}.
CC   -!- SUBUNIT: Binds directly or indirectly to the Woronin body major protein
CC       hexA. {ECO:0000305|PubMed:23869404}.
CC   -!- SUBCELLULAR LOCATION: Cell septum {ECO:0000269|PubMed:23869404}.
CC       Note=Localizes to the central part of the septum indicating a specific
CC       targeting to the rim of the septal pore. {ECO:0000269|PubMed:23869404}.
CC   -!- DOMAIN: The N-terminal domain binds to Woronin bodies in the cytoplasm
CC       and on either side of the septal pore, whereas the C-terminal domain
CC       binds to the septal pore. {ECO:0000269|PubMed:23869404}.
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DR   EMBL; AAHF01000001; EAL93103.1; -; Genomic_DNA.
DR   RefSeq; XP_755141.1; XM_750048.1.
DR   STRING; 330879.Q4X212; -.
DR   EnsemblFungi; EAL93103; EAL93103; AFUA_2G08060.
DR   GeneID; 3513060; -.
DR   KEGG; afm:AFUA_2G08060; -.
DR   VEuPathDB; FungiDB:Afu2g08060; -.
DR   eggNOG; ENOG502QRYC; Eukaryota.
DR   HOGENOM; CLU_000055_0_0_1; -.
DR   InParanoid; Q4X212; -.
DR   OMA; MMRTPKV; -.
DR   OrthoDB; 158164at2759; -.
DR   Proteomes; UP000002530; Chromosome 2.
DR   GO; GO:0000934; C:porous cell septum; IDA:AspGD.
DR   GO; GO:0009611; P:response to wounding; IMP:AspGD.
PE   1: Evidence at protein level;
KW   Coiled coil; Reference proteome; Virulence.
FT   CHAIN           1..5538
FT                   /note="Leashin"
FT                   /id="PRO_0000455966"
FT   REGION          1..1100
FT                   /note="Woronin bodies-binding region"
FT                   /evidence="ECO:0000269|PubMed:23869404"
FT   REGION          4495..5538
FT                   /note="Septal pore-binding region"
FT                   /evidence="ECO:0000269|PubMed:23869404"
FT   COILED          5050..5223
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   5538 AA;  603726 MW;  B2EA8B086BBB63AE CRC64;
     MFRALMGGGR SSDSRSTTSS SKSSSRRRTN SKASSTVSRK SSRGDDRDRG LGDLSAYSFS
     GSRSKRYAPS AAGDSVASSY ATAEPGIAVE HDRIIIERTL KRRDTDEESG RDRYSEIRDR
     DGRSSRRRDR DRSQSRERER PRSERIERAQ LEDDAVESRD RRRERSRTQP GDTYLPPVSP
     SMPIQPNSPL VYDPHVQQQF PGQFPAYVAE PYRPPNPAGE AADYYGDQGQ SVADQPGVRP
     KPPPIIPSSQ AHLMTASPVA NPPPEPSSMG QVGAAAAYFA DDAELEVDPA PGRPDRPTAG
     TTSEPPKPSN TTFGISGIAA GAAAYGAGGS LPLPASPTSP PEPVPTTAPY APPVTSSTTK
     PPHTHGIGAS VGAAAAGAAA GYMLGHHQHH SMSSADHLSQ YTMQNYDESS QYGLGIPGPT
     VYNAPANAEW HAAGTGTAVP YAASPLHPHH AAVHHGAPFP SGSLAFQQRQ RGPLDKFIDF
     WRDPEGVGMF EEYTEAIGVC KYCFEPGTSS RDAPRKHHYR QRRSSGERYA SGSRVGKASR
     YTSSEDEGRR RKKSSRNSWL LPGLLGGIAA KALFNNKDFE DTYSVRSGRV MTVKDTESVS
     TARRSQTSRG VYRRRSQSRD RESRIIYSDS KSQYEDKRHR SRSRSHSSSR NRRHSALRDA
     AVGAAVGSGA IALAKSRDRS RSRSRSRSRF PRKSKGRKSS TSDSSSFLDI SQPARKSVGG
     GIASFFTASS ENRGKRRVKK RRSIFSFNNS SSSSLDADLA FGTGFARKPA RKSTKKSSKK
     KDRDVDAALL GLGAAATALA ATSHRRSRRA GEILVAKETR SRHSDYASSV TNDEGWEDLD
     SGDQSSSSVS SALAFGDTGL FGSDESQSSD SGTSKWGWRW GSKKNKKKKR ASSPQGRFPT
     GAALAAGALG TAALASTQDR DSRLPRQHAS SSSGSLQHVA PVPTNDPTLV DAVRVASLPH
     AEPAFVRPGP IPLQQPQPMT PVSQAVYATQ GASIPTYAAP MAPPAFPPTS YIPYRPEQIQ
     NVALGFNRPH HRSDSSPVFH TEPLEGVPAP GLKRRSTSKD QSSVQFDLTK EQADKERRMD
     RLEQLKRDAE RASGVQLIDR DHEPTVRDDD RRSGRYEDRG YVDRRQDEPR EDRYAMDSGK
     DKDSFSRVGA VAAGSIGAAA AATVLSGRSS VDESSETSQR RHEERRQQRR AERRRGSEPE
     SAVSSRSKSE RAQETTDYLP EERQPEPTKP PSPSRSPHKY DDYAEFFAPE ELRYSPDTYK
     QRAPTSMPTI VEIEPASERQ SREALLPAEE SHPGYRDLPW PVPVLKLIEP TPPQSVSGSV
     RDAASPVGNP RDLPPHEEEE DVKPAARQTT GSRVSWGEHK THEYEVPSTS SELESVDHET
     TREREQPHSP VLQQRYVSPK NAADDVGADI EFAAALAAAT AAAGFNPALV TEDPTYHTWS
     SPPGSHGRVE YRDPWVETES KSRIPHGFVE GEVETPEEEK APSSRVIEEQ PLYSEPEPVS
     REPESQESSE PQTRTSIAQE VIDRLSEKQD ERDGSRKALY GTEKSSNSGK ERDESELRAQ
     DSFSMPGGFE TEELRSDPKR DVDSRDDGDV DRRSVASAPV SGEYDFSTRP RKSTQDSEYF
     DNGEDAGSAS IEQDGSEGKK KRRKRRSKRD SDTFTDSASV ESSPARIGQS SEKLKSMDDK
     DKEKKAGGFF SSIFGSRVSE PVDSKRSSST DRPSRDVHSE IGRREYEESR RQRKEEKSSR
     RDEESGSDKE NSKVRDKDGV DIENYKSSRQ RREERRRRRY EDIVDSGKSG EYEKDRKLSE
     DNDENQSFLA EGPEMPAQIG DGDRGSGASG HVRLAEGAIT GLGIGVLGQR PRGRSTPPEA
     SERIMDPAPR SRSRPASPEP DRQEGDNQSQ SSRRSSILRS KDSPTAVPLH FRRPPASPGT
     NRSVSVGTPT APSPGSPTTP KRRPNSTEFK NSREMRPLWL VERHGPGHGE HKLEEPLPSL
     PSSKTSSANT SVEDLTALQD ERSWEAVDLS HHVHGMRRLS GIDVSQSRGF EHDAFGSQHV
     TPTATTFEQI HPHSRKEKLK YEFHSPSELL QDPSPYGDVQ PSNMGDLPSA EGSAVGVKDA
     SSENEDSAEL AAEALPPRPS TPQNNVTAAS EDTETTPTQT RTVNAFEGPG FAGVVDAAVA
     AAVSNRLSTR PDVAIPDKSS PEDLPYDADR THKPIADQEL AATSPPAPGV PLGFAAVVDA
     AVAAATISIG GQPEAAKELE QSPEISEPIP QTPHPNEQET SHPTGNDSVP RDDKRRDSVD
     TVVPQAEEAS DEKEKLDTSA VMPDLTGENK ELPSEAKNEN ANDNSQAQTE QPPVDTDEPS
     SSSAKKKKKK NKKKRQSMDS NTQEPTTPVD DSTIDQGGVG ISAVEDAQAD AVETFEPAPK
     EQILVEQPAS AEPTPAPEPE PTETTVDVEK AIEAPDVRKE LEEEPAPAAP EDTPAEPTAE
     TPAEDQAETS SSKKSKKKKK KKNKGSAPEE NTEDPASTET PEASAANSQV VAAEQVESTL
     ETTQPAEEEP KPTEESAIAV AENTVEPEPA PKEMEATEDP MFQDISVPGE SQDSPADADN
     QAKELPHPEE EFQAEATHAQ ELPEDTEIIN KTDDLSSGID PSGQAGGDMV NEAASPEVWH
     DALASSPEDK NGEAEQADLK SNEPQNEGKA PVSELPALDK ELSEISERPA EVDAPADTPL
     VDSPSTDQVE AADSGVQNEE PTPTAAELET PLSRKNSKKN KKKNKRKNTA ETPVQNEAVH
     TADPISSIEG VPEPGPEATT TAVEEPQVTL PDEAVDENKG ESRDVQAVKE ETSPENAAEV
     ANDSQPSTFE EIHLAGAEQS APDAIAEIMG ESQPAEPQDV LATAPEEPID GQPKKKAKKK
     KNRKTANVSE SQPESEAEAK TEKLQSAELA ENPQPHIRDE VAGDSQVPPE SGASEAPAGI
     EDVTPISAAE AVEFDLPVEK DETNGGEPHV SELNKQLNNE TVPGPETGSE PVPEAGEITQ
     SGKKSKKNKK KKQSLSLAPD ETPASDPSTP AGTADGNADL PAAPEDSLKT DQEPMPEEPT
     VSQPIVDLVT ETLNLSMAEE AVPMTAAQKK TKKEKKKKRQ SALLDEPTAT ESIEEANAKD
     VTSEGTQMPL EVPSEPQSSG PTLDAIEHAE AAAEHSQEQP NKDVTLHADH SPNSDGEFVL
     VPEHVPYGSN DEHKTQPGSM ELDVTQVNTE LEKEPSTQEG VLEANEATPA ETPSAADQHV
     QEESSPTPAM EGGAAAEELV AVEPDVLEGS QDKITEDNDT PDDSLTAKEP QTELVNAETT
     QKTEQGDVVL DVEAGSEGLV RDDQPVAPSK KKDKKKKKKR QSLTIDDEQR SSTKEEPTAE
     FSSDHVPEPS AVDESATTPS ASEEQQKPET DITETVTQTA AEPTPSSASE EPENIAEAPS
     NESTQEPAAE EAQTAKSKKK AKKDKKKRKS VSFEIGEPLT QQSEPGHPTA TPGETVTPHE
     GPKPGDKPTS PKDSSEEFQS GEAVPESPQD SAGIVTQPEQ PEPTAEATVV TEQHKQVTEP
     SLVSINEEQA VVEETVAPPV VDEASQLQEQ KVSSETLWSE TQRDVVKSFQ DAESLEEEKG
     ETAVSPSLEN NEGTKPGAWV EQAEFEPQTP TDDGEQGVGP SKSKKNKKKK KRNTLESTED
     VPVIPPGPSK IELLESTPVL ETEETHDIVE PDTTVSENVH EEEKPEDEFG GFMSAKAKKK
     AKKKDKKRQS KILDSANDAT NTAESAPDIS EQAPLETASG EADGPDATFS QETSETISTE
     AKSSEPSPET ALTPAEDDGK ENQSHDTEPH GGNDKDLTWT DHMVSSQVEQ QQGTPSDRPS
     EPAPETEPIS TREAATSIDV EQLDNNADDA SPAVNDRLER SGEEGTRVKK EIVSEEPEVQ
     YEGLLEIPRE EKAEISSQGE DTIQVKSDAE VEESVKATVE GTPEDSMSQE DAPISVLDGG
     DATTKDQFTS IEVNDPSQSK DILEPENEEL PLPIPGKKKK AQQETKIDET SQDDSVDAVQ
     EESPTSREMT DTGLPVAESQ ADPIAEPFHE LPEPQKAVEI AVEDKSMQET LKFKQDVTPA
     EDVFQEPPAL GRKKSKKDKK KDLLAQNTAV ESRENKFKEK QLATEEPLIT PSDARTTVLA
     TPVFTQALET PTKTTVDAGE RELHLSAVQS TVPVEDNINA LETSGAQTLE SFRTLEAPEQ
     PLERNILLND YAAKTPEELP AVEKSATYET ATEPIIEEAA LSHKNSKKKS KKAKKQAQEQ
     QEKSTTPTPA EHGEESIVET TAAIPSTPGP AENSDDIPEA NISEEQVAQL EHSEQLVLSD
     AARPQERLGQ TPNMDNQTDD VQSTEQGKEG NVQAEPRQVE SEDPIESQPG STAAVARKLS
     KKDRRKAKKK SAKDAIEPSD EPELRNPTEP IGASCSTSNQ AKIDQDQFLT VASDKQMVEE
     VPRPLEVEPA ASEVYQGATD ENDWPAIDWE KGKVEFKEQT PLSSPEAHAV PFEPAIAEFD
     ETAIPEGLLR RQSLSREEQL AGGKDGSSQH TRADEIAAPQ EGAVMIEPSA VAETEQSAGL
     QAKSVSSQGA PKTIQDDMQH PENRLARDQT KSETGVVPSK QSKIGSIFPD LERGSFRRPV
     PGQVLMPVKD RAEDETIDQN ADDNSAIKVS EAPIPAGEPE ESHLQSQQDE KGPEPTTTVS
     TLDLSLEEPT KMQDTSNTPV NLAVDIEVDP SYNVSVISDG LVNETKSIEI EWKTDGDKGT
     QEAETPLYAP LPVHEQKSSL VSQASPLDMD RDEPIPRNDS SCGLRRSPSI HGRHNHPPRT
     WSLEDTPITK AVTPPLFGGP VGATADMSSP PRTPLQPIAE QEPEVRVEQA SGFRSMVSEH
     GTPRLEMKPE HVLPRPETPI RKFTDNALAR QTWPVAENDI FKSSEDEDAA LTVKKQRPGN
     KWPAEVLKTP DKGMPILRPS SVSSVKSVQS THSVTGGQRS LRRTSRNTSG DLRAASQAQE
     SHGTQPHATP QPPQPPPSDL NIEHIASSSS YDPVTDKGKR PIRAMTDVYE GWGESPSSPR
     SPSRPPSIRH RRSMQHLQEL EARLDQLISE NRLLIAAREA AEDKLRNASV ARRKSDHTLN
     ERSADLRDRE AEVEKLKKSV EWLQKEVTRL TEENEGLITT NSNLTAAHAK EIESVRESSS
     RQLDDLRSRY EQLSMEVQNT VRHEIETALA RKDNELRRLR EELETARDKV SQLQQQIAAS
     LHDNVLVFRD EDYFDAACQK LCGHVQQWVL RFSKHSDHRR CRKLAEIQDE KIADRFDNAI
     LDGYDTDTYL ADRVRRRDIF MSVVMTMVWE FVFTRYLFGM DREQRQKLKS LEKQLNEVGP
     RSAVHRWRAI TLTLLSKRPA FARQRESDTE AVALEIFETL SRLLPPPSHV EVQLLESLRK
     VLRVAVNLSI EMRTQLAEYI MLPPLQPEYD TNGDLARQVY FNASLMNERS GETTSNEELE
     SQQAVVRVVL FPLVVKKGND TGEGEDEVVV CPAQVLVARP DKDPRASKIF SSDRMSLDGT
     KSVHSVAPSS TMDISNVI