ID   ARCH_ICTPU              Reviewed;         162 AA.
AC   Q2EGP9;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Protein archease;
OS   Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Ictaluridae; Ictalurus.
OX   NCBI_TaxID=7998;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RA   Yeh H.-Y., Klesius P.H.;
RT   "Channel catfish gene expression after Edwardsiella ictaluri infection.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the tRNA-splicing ligase complex required to
CC       facilitate the enzymatic turnover of catalytic subunit RTCB. Together
CC       with ddx1, acts by facilitating the guanylylation of RTCB, a key
CC       intermediate step in tRNA ligation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the tRNA-splicing ligase complex. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the archease family. {ECO:0000305}.
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DR   EMBL; DQ381261; ABD38668.1; -; mRNA.
DR   RefSeq; NP_001187153.1; NM_001200224.1.
DR   AlphaFoldDB; Q2EGP9; -.
DR   SMR; Q2EGP9; -.
DR   STRING; 7998.ENSIPUP00000029176; -.
DR   GeneID; 108263438; -.
DR   KEGG; ipu:100304789; -.
DR   CTD; 339487; -.
DR   Proteomes; UP000221080; Genome assembly.
DR   GO; GO:0072669; C:tRNA-splicing ligase complex; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; ISS:UniProtKB.
DR   Gene3D; 3.55.10.10; -; 1.
DR   InterPro; IPR002804; Archease.
DR   InterPro; IPR023572; Archease_dom.
DR   InterPro; IPR036820; Archease_dom_sf.
DR   PANTHER; PTHR12682; PTHR12682; 1.
DR   Pfam; PF01951; Archease; 1.
DR   SUPFAM; SSF69819; SSF69819; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Metal-binding; tRNA processing.
FT   CHAIN           1..162
FT                   /note="Protein archease"
FT                   /id="PRO_0000285953"
FT   BINDING         34
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         161
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         162
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   162 AA;  18799 MW;  E90EC9CB12DFE5D9 CRC64;
     MNDRELDLTE AQKAIKAKYP AINKKYEYLD HTADVQLHSW GDTLEEAFEQ CAMAMFGYMT
     DTETVEPIDT IEIESEGDDL ESLLFHFLDD WLFKFSADAF FVPREVKVLQ LDRMRFTIRS
     IGWGEEFNLA KHPQGTEVKA ITYSAMQICD KEKPEIFAII DI