ARCH_METBF
ID ARCH_METBF Reviewed; 146 AA.
AC Q46D27;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Protein archease {ECO:0000255|HAMAP-Rule:MF_01222};
GN OrderedLocusNames=Mbar_A1251;
OS Methanosarcina barkeri (strain Fusaro / DSM 804).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=269797;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Fusaro / DSM 804;
RX PubMed=16980466; DOI=10.1128/jb.00810-06;
RA Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT "The Methanosarcina barkeri genome: comparative analysis with
RT Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT rearrangement within methanosarcinal genomes.";
RL J. Bacteriol. 188:7922-7931(2006).
CC -!- FUNCTION: Activates the tRNA-splicing ligase complex by facilitating
CC the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the
CC guanylylation of RtcB, a key intermediate step in tRNA ligation. Can
CC also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is
CC used efficiently (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the archease family. {ECO:0000255|HAMAP-
CC Rule:MF_01222}.
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DR EMBL; CP000099; AAZ70215.1; -; Genomic_DNA.
DR RefSeq; WP_011306262.1; NC_007355.1.
DR AlphaFoldDB; Q46D27; -.
DR SMR; Q46D27; -.
DR STRING; 269797.Mbar_A1251; -.
DR EnsemblBacteria; AAZ70215; AAZ70215; Mbar_A1251.
DR GeneID; 3627851; -.
DR KEGG; mba:Mbar_A1251; -.
DR eggNOG; arCOG04055; Archaea.
DR HOGENOM; CLU_111362_3_0_2; -.
DR OMA; WLSELLY; -.
DR OrthoDB; 125788at2157; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.55.10.10; -; 1.
DR HAMAP; MF_01222; Archease_arch; 1.
DR InterPro; IPR002804; Archease.
DR InterPro; IPR022952; Archease_arc.
DR InterPro; IPR023572; Archease_dom.
DR InterPro; IPR036820; Archease_dom_sf.
DR PANTHER; PTHR12682; PTHR12682; 1.
DR Pfam; PF01951; Archease; 1.
DR SUPFAM; SSF69819; SSF69819; 1.
PE 3: Inferred from homology;
KW Calcium; Metal-binding; tRNA processing.
FT CHAIN 1..146
FT /note="Protein archease"
FT /id="PRO_1000066781"
FT BINDING 16
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 145
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 146
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
SQ SEQUENCE 146 AA; 16846 MW; 6C30D30365EA9237 CRC64;
MSSQGKQYEY LDHTADIKFQ AYGKTREEVF ENAALAMFNV IIDTKKISGD TAREIFLKSP
DLESLLVDWL SELLYLFEVD EIVFREFRVD NIREENGEYS ITAQALGEKY DLKSLPFETE
IKAVTYNQLE ITKTADGWKA QVVVDI