ID   LAN2B_RUMFL             Reviewed;          67 AA.
AC   P0DQL4;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-AUG-2020, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Lantibiotic Flvbeta.b {ECO:0000303|PubMed:27028884};
DE   Flags: Precursor;
GN   Name=FlvA2.b {ECO:0000303|PubMed:27028884};
OS   Ruminococcus flavefaciens.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminococcus.
OX   NCBI_TaxID=1265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION IN E.COLI, DEHYDRATION AT
RP   SER-36; THR-39 AND THR-43, AND METHYLLANTHIONINE CROSS-LINKS.
RC   STRAIN=FD-1;
RX   PubMed=27028884; DOI=10.1016/j.chembiol.2015.11.014;
RA   Zhao X., van der Donk W.A.;
RT   "Structural characterization and bioactivity analysis of the two-component
RT   lantibiotic Flv system from a ruminant bacterium.";
RL   Cell Chem. Biol. 23:246-256(2016).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic) only
CC       active on Gram-positive bacteria in synergy with Flvalpha.a
CC       (PubMed:27028884). Is not active in absence of Flvalpha.a, which is
CC       encoded by the same operon than Flvbeta.b (PubMed:27028884). The
CC       bactericidal activity of lantibiotics is based on depolarization of
CC       energized bacterial cytoplasmic membranes, initiated by the formation
CC       of aqueous transmembrane pores (By similarity).
CC       {ECO:0000250|UniProtKB:P86475, ECO:0000269|PubMed:27028884}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- PTM: Contains DL-beta-methyllanthionine, when coepressed in E.coli with
CC       the flavecin synthetase FlvM2. {ECO:0000269|PubMed:27028884}.
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DR   AlphaFoldDB; P0DQL4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial; Bacteriocin; Lantibiotic; Secreted;
KW   Thioether bond.
FT   PROPEP          1..34
FT                   /note="Cleaved by FlvT"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT                   /id="PRO_0000450398"
FT   PEPTIDE         35..67
FT                   /note="Lantibiotic Flvbeta.b"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT                   /id="PRO_0000450399"
FT   MOD_RES         36
FT                   /note="2,3-didehydroalanine (Ser); by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT   MOD_RES         39
FT                   /note="2,3-didehydrobutyrine; by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT   MOD_RES         43
FT                   /note="2,3-didehydrobutyrine; by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT   CROSSLNK        50..56
FT                   /note="Beta-methyllanthionine (Thr-Cys); by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT   CROSSLNK        58..61
FT                   /note="Beta-methyllanthionine (Thr-Cys); by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
FT   CROSSLNK        62..65
FT                   /note="Beta-methyllanthionine (Thr-Cys); by FlvM2"
FT                   /evidence="ECO:0000305|PubMed:27028884"
SQ   SEQUENCE   67 AA;  7089 MW;  AABCC51792157B59 CRC64;
     MDNNTKLQKL YEQLAATGSE KELDAMLDEN MAGAGSPLTV TITGLIVAAT TGFDWCPTGA
     CTYSCRV