LAN2F_RUMFL
ID LAN2F_RUMFL Reviewed; 59 AA.
AC P0DQL8;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 12-AUG-2020, sequence version 1.
DT 25-MAY-2022, entry version 4.
DE RecName: Full=Lantipeptide Flvbeta.f {ECO:0000303|PubMed:27028884};
DE Flags: Precursor;
GN Name=FlvA2.f {ECO:0000303|PubMed:27028884};
OS Ruminococcus flavefaciens.
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=1265;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION IN E.COLI, DEHYDRATION AT
RP THR-31 AND THR-32, AND METHYLLANTHIONINE CROSS-LINKS.
RC STRAIN=FD-1;
RX PubMed=27028884; DOI=10.1016/j.chembiol.2015.11.014;
RA Zhao X., van der Donk W.A.;
RT "Structural characterization and bioactivity analysis of the two-component
RT lantibiotic Flv system from a ruminant bacterium.";
RL Cell Chem. Biol. 23:246-256(2016).
CC -!- FUNCTION: Lanthionine-containing peptide that does probably not show
CC antibacterial activity, since its analog [+7]Flvbeta.f does not show
CC antibacterial activity against M.luteus (PubMed:27028884). Also does
CC not show antibiotic activity when tested with [Del2]Flvalpha.a, an
CC analog of Flvalpha.a, which is encoded by the same operon than
CC Flvbeta.f (PubMed:27028884). The bactericidal activity of lantibiotics
CC is based on depolarization of energized bacterial cytoplasmic
CC membranes, initiated by the formation of aqueous transmembrane pores
CC (By similarity). {ECO:0000250|UniProtKB:P86475,
CC ECO:0000269|PubMed:27028884}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- PTM: Contains DL-beta-methyllanthionine, when coepressed in E.coli with
CC the flavecin synthetase FlvM2. {ECO:0000269|PubMed:27028884}.
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DR AlphaFoldDB; P0DQL8; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 3: Inferred from homology;
KW Secreted; Thioether bond.
FT PROPEP 1..27
FT /note="Cleaved by FlvT"
FT /evidence="ECO:0000305|PubMed:27028884"
FT /id="PRO_0000450406"
FT PEPTIDE 28..59
FT /note="Lantipeptide Flvbeta.f"
FT /evidence="ECO:0000305|PubMed:27028884"
FT /id="PRO_0000450407"
FT MOD_RES 31
FT /note="2,3-didehydrobutyrine; by FlvM2"
FT /evidence="ECO:0000305|PubMed:27028884"
FT MOD_RES 32
FT /note="2,3-didehydrobutyrine; by FlvM2"
FT /evidence="ECO:0000305|PubMed:27028884"
FT CROSSLNK 41..47
FT /note="Beta-methyllanthionine (Thr-Cys); by FlvM2"
FT /evidence="ECO:0000305|PubMed:27028884"
FT CROSSLNK 53..56
FT /note="Beta-methyllanthionine (Thr-Cys); by FlvM2"
FT /evidence="ECO:0000305|PubMed:27028884"
SQ SEQUENCE 59 AA; 6560 MW; 458EA1600268BF7B CRC64;
MEKMNNIAGI TPENELDEMF DDSVVGAVGY TTYWGILPLV TKNPQICPVS ENTVKCRLL