ID   LAN4_LACLL              Reviewed;          51 AA.
AC   P36499;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Lantibiotic lacticin-481;
DE   AltName: Full=Lactococcin-DR;
DE   Flags: Precursor;
GN   Name=lctA; Synonyms=lcnDR1, lct;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRUCTURE BY NMR, DEHYDRATION AT THR-48,
RP   AND LANTHIONINE CROSS-LINKS.
RC   STRAIN=CNRZ 481;
RX   PubMed=8344922; DOI=10.1016/s0021-9258(19)85429-7;
RA   Piard J.-C., Kuipers O.P., Rollema H.S., Desmazeaud M.J., de Vos W.M.;
RT   "Structure, organization, and expression of the lct gene for lacticin 481,
RT   a novel lantibiotic produced by Lactococcus lactis.";
RL   J. Biol. Chem. 268:16361-16368(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ADRIA 85LO30;
RX   PubMed=8017945; DOI=10.1128/aem.60.5.1652-1657.1994;
RA   Rince A., Dufour A., le Pogam S., Thuault D., Bourgeois C.M., Pennec J.P.;
RT   "Cloning, expression, and nucleotide sequence of genes involved in
RT   production of lactococcin DR, a bacteriocin from Lactococcus lactis subsp.
RT   lactis.";
RL   Appl. Environ. Microbiol. 60:1652-1657(1994).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-31.
RC   STRAIN=CNRZ 481;
RX   PubMed=16348628; DOI=10.1128/aem.58.1.279-284.1992;
RA   Piard J.-C., Muriana P.M., Desmazeaud M.J., Klaenhammer T.R.;
RT   "Purification and partial characterization of lacticin 481, a lanthionine-
RT   containing bacteriocin produced by Lactococcus lactis subsp. lactis CNRZ
RT   481.";
RL   Appl. Environ. Microbiol. 58:279-284(1992).
RN   [4]
RP   THIOETHER BONDS.
RX   PubMed=8764998; DOI=10.1016/0014-5793(96)00771-5;
RA   van den Hooven H.W., Lagerwerf F.M., Heerma W., Haverkamp J., Piard J.-C.,
RA   Hilbers C.W., Siezen R.J., Kuipers O.P., Rollema H.S.;
RT   "The structure of the lantibiotic lacticin 481 produced by Lactococcus
RT   lactis: location of the thioether bridges.";
RL   FEBS Lett. 391:317-322(1996).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC       active on Gram-positive bacteria. The bactericidal activity of
CC       lantibiotics is based on depolarization of energized bacterial
CC       cytoplasmic membranes, initiated by the formation of aqueous
CC       transmembrane pores. Lacticin 481 is a broad spectrum bacteriocin
CC       exhibiting activity against a wide range of lactic acid bacteria and
CC       C.tyrobutyricum.
CC   -!- SUBUNIT: Monomer or homodimer.
CC   -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC       Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC       with cysteine. This is followed by membrane translocation and cleavage
CC       of the modified precursor.
CC   -!- PTM: It is established that the 2,3-didehydrobutyrine is the Z-isomer.
CC       {ECO:0000269|PubMed:8344922}.
CC   -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000305}.
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DR   EMBL; X71410; CAA50534.1; -; Genomic_DNA.
DR   EMBL; U91581; AAC72257.1; -; Genomic_DNA.
DR   PIR; A47342; A47342.
DR   AlphaFoldDB; P36499; -.
DR   SMR; P36499; -.
DR   TCDB; 1.C.21.1.1; the lacticin 481 (lacticin 481) family.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR007682; Lantibiotic_typ-A_Lactobact.
DR   Pfam; PF04604; L_biotic_typeA; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Lantibiotic; Thioether bond.
FT   PROPEP          1..24
FT                   /evidence="ECO:0000269|PubMed:16348628"
FT                   /id="PRO_0000017120"
FT   PEPTIDE         25..51
FT                   /note="Lantibiotic lacticin-481"
FT                   /id="PRO_0000017121"
FT   MOD_RES         48
FT                   /note="(Z)-2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:8344922"
FT   CROSSLNK        33..38
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:8344922"
FT   CROSSLNK        35..49
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:8344922,
FT                   ECO:0000269|PubMed:8764998"
FT   CROSSLNK        42..50
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:8344922,
FT                   ECO:0000269|PubMed:8764998"
SQ   SEQUENCE   51 AA;  5677 MW;  44A3951AC2CEAE3D CRC64;
     MKEQNSFNLL QEVTESELDL ILGAKGGSGV IHTISHECNM NSWQFVFTCC S