LANA1_LACLL
ID LANA1_LACLL Reviewed; 59 AA.
AC O87236;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Lantibiotic lacticin 3147 A1;
DE Flags: Precursor;
GN Name=ltnA1 {ECO:0000303|PubMed:10608807};
GN Synonyms=ltnA {ECO:0000312|EMBL:AAF32256.1}; ORFNames=ORF00035;
OS Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG Plasmid pMRC01 {ECO:0000312|EMBL:AAC56053.1}, and
OG Plasmid pBAC105 {ECO:0000312|EMBL:AAF32256.1}.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1360;
RN [1] {ECO:0000312|EMBL:AAC56053.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DPC3147 {ECO:0000312|EMBL:AAC56053.1}; PLASMID=pMRC01;
RX PubMed=9767571; DOI=10.1046/j.1365-2958.1998.00988.x;
RA Dougherty B.A., Hill C., Weidman J.F., Richardson D.R., Venter J.C.,
RA Ross R.P.;
RT "Sequence and analysis of the 60 kb conjugative, bacteriocin-producing
RT plasmid pMRC01 from Lactococcus lactis DPC3147.";
RL Mol. Microbiol. 29:1029-1038(1998).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF32256.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=IFPL105 {ECO:0000312|EMBL:AAF32256.1}; PLASMID=pBAC105;
RX PubMed=10971756; DOI=10.1046/j.1365-2672.2000.01103.x;
RA Martinez-Cuesta M.C., Buist G., Kok J., Hauge H.H., Nissen-Meyer J.,
RA Pelaez C., Requena T.;
RT "Biological and molecular characterization of a two-peptide lantibiotic
RT produced by Lactococcus lactis IFPL105.";
RL J. Appl. Microbiol. 89:249-260(2000).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 30-59, MASS SPECTROMETRY, THIOETHER BONDS, AND
RP DEHYDRATION AT THR-32; THR-34 AND SER-36.
RC STRAIN=DPC3147 {ECO:0000269|PubMed:15023056};
RX PubMed=15023056; DOI=10.1021/bi0362065;
RA Martin N.I., Sprules T., Carpenter M.R., Cotter P.D., Hill C., Ross R.P.,
RA Vederas J.C.;
RT "Structural characterization of lacticin 3147, a two-peptide lantibiotic
RT with synergistic activity.";
RL Biochemistry 43:3049-3056(2004).
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 30-47, FUNCTION, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=DPC3147 {ECO:0000269|PubMed:10608807};
RX PubMed=10608807; DOI=10.1074/jbc.274.53.37544;
RA Ryan M.P., Jack R.W., Josten M., Sahl H.-G., Jung G., Ross R.P., Hill C.;
RT "Extensive post-translational modification, including serine to D-alanine
RT conversion, in the two-component lantibiotic, lacticin 3147.";
RL J. Biol. Chem. 274:37544-37550(1999).
CC -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC active on Gram-positive bacteria. The bactericidal activity of
CC lantibiotics is based on depolarization of energized bacterial
CC cytoplasmic membranes, initiated by the formation of aqueous
CC transmembrane pores. When present individually lacticin 3147 A1
CC exhibits strong activity towards L.lactis strain AM2, weak activity
CC towards L.lactis strain HP and no activity towards L.lactis strain
CC IFPL359, but when combined with lacticin 3147 A2 it displays strong
CC activity towards all three strains. {ECO:0000269|PubMed:10608807,
CC ECO:0000269|PubMed:10971756}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10608807,
CC ECO:0000269|PubMed:10971756}.
CC -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC with cysteine. This is followed by membrane translocation and cleavage
CC of the modified precursor. {ECO:0000269|PubMed:15023056}.
CC -!- PTM: It is not established whether the 2,3-didehydrobutyrines are the
CC E- or Z-isomers (PubMed:15023056 and PubMed:10608807). In the NMR model
CC they were assumed to be the Z-isomer.
CC -!- MASS SPECTROMETRY: Mass=3322; Method=Plasma desorption;
CC Evidence={ECO:0000269|PubMed:10971756};
CC -!- MASS SPECTROMETRY: Mass=3306.69; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15023056};
CC -!- MASS SPECTROMETRY: Mass=3322.34; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10608807};
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DR EMBL; AE001272; AAC56053.1; -; Genomic_DNA.
DR EMBL; AF167432; AAF32256.1; -; Genomic_DNA.
DR PIR; T43106; T43106.
DR RefSeq; NP_047319.1; NC_001949.1.
DR AlphaFoldDB; O87236; -.
DR TCDB; 1.C.21.2.4; the lacticin 481 (lacticin 481) family.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR InterPro; IPR029243; Lantibiotic_alpha.
DR Pfam; PF14867; Lantibiotic_a; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW Lantibiotic; Plasmid; Secreted; Thioether bond.
FT PROPEP 1..29
FT /evidence="ECO:0000269|PubMed:10608807,
FT ECO:0000269|PubMed:15023056"
FT /id="PRO_0000042849"
FT PEPTIDE 30..59
FT /note="Lantibiotic lacticin 3147 A1"
FT /evidence="ECO:0000269|PubMed:15023056"
FT /id="PRO_0000042850"
FT MOD_RES 32
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 34
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 36
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 30..31
FT /note="Lanthionine (Cys-Ser)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 38..48
FT /note="Lanthionine (Ser-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 49..54
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 51..58
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
SQ SEQUENCE 59 AA; 6798 MW; 850B313769976D42 CRC64;
MNKNEIETQP VTWLEEVSDQ NFDEDVFGAC STNTFSLSDY WGNNGAWCTL THECMAWCK
