LANA2_LACLL
ID LANA2_LACLL Reviewed; 65 AA.
AC O87237;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=Lantibiotic lacticin 3147 A2;
DE Flags: Precursor;
GN Name=ltnA2 {ECO:0000303|PubMed:10608807};
GN Synonyms=ltnB {ECO:0000312|EMBL:AAF32257.1}; ORFNames=ORF00036;
OS Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG Plasmid pMRC01 {ECO:0000312|EMBL:AAC56054.1}, and
OG Plasmid pBAC105 {ECO:0000312|EMBL:AAF32257.1}.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1360;
RN [1] {ECO:0000312|EMBL:AAC56054.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DPC3147 {ECO:0000312|EMBL:AAC56054.1}; PLASMID=pMRC01;
RX PubMed=9767571; DOI=10.1046/j.1365-2958.1998.00988.x;
RA Dougherty B.A., Hill C., Weidman J.F., Richardson D.R., Venter J.C.,
RA Ross R.P.;
RT "Sequence and analysis of the 60 kb conjugative, bacteriocin-producing
RT plasmid pMRC01 from Lactococcus lactis DPC3147.";
RL Mol. Microbiol. 29:1029-1038(1998).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF32257.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 51-57, FUNCTION,
RP SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC STRAIN=IFPL105 {ECO:0000312|EMBL:AAF32257.1}; PLASMID=pBAC105;
RX PubMed=10971756; DOI=10.1046/j.1365-2672.2000.01103.x;
RA Martinez-Cuesta M.C., Buist G., Kok J., Hauge H.H., Nissen-Meyer J.,
RA Pelaez C., Requena T.;
RT "Biological and molecular characterization of a two-peptide lantibiotic
RT produced by Lactococcus lactis IFPL105.";
RL J. Appl. Microbiol. 89:249-260(2000).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 37-65, MASS SPECTROMETRY, THIOETHER BONDS, AND
RP DEHYDRATION AT THR-38; THR-41; SER-45 AND SER-48.
RC STRAIN=DPC3147 {ECO:0000269|PubMed:15023056};
RX PubMed=15023056; DOI=10.1021/bi0362065;
RA Martin N.I., Sprules T., Carpenter M.R., Cotter P.D., Hill C., Ross R.P.,
RA Vederas J.C.;
RT "Structural characterization of lacticin 3147, a two-peptide lantibiotic
RT with synergistic activity.";
RL Biochemistry 43:3049-3056(2004).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC STRAIN=DPC3147 {ECO:0000269|PubMed:10608807};
RX PubMed=10608807; DOI=10.1074/jbc.274.53.37544;
RA Ryan M.P., Jack R.W., Josten M., Sahl H.-G., Jung G., Ross R.P., Hill C.;
RT "Extensive post-translational modification, including serine to D-alanine
RT conversion, in the two-component lantibiotic, lacticin 3147.";
RL J. Biol. Chem. 274:37544-37550(1999).
CC -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC active on Gram-positive bacteria. The bactericidal activity of
CC lantibiotics is based on depolarization of energized bacterial
CC cytoplasmic membranes, initiated by the formation of aqueous
CC transmembrane pores. When present individually lacticin 3147 A2
CC exhibits weak activity towards L.lactis strain AM2 and L.lactis strain
CC HP, and no activity towards L.lactis strain IFPL359, but when combined
CC with lacticin 3147 A1 it displays strong activity towards all three
CC strains. {ECO:0000269|PubMed:10608807, ECO:0000269|PubMed:10971756}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10608807,
CC ECO:0000269|PubMed:10971756}.
CC -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC with cysteine. This is followed by membrane translocation and cleavage
CC of the modified precursor. {ECO:0000269|PubMed:15023056}.
CC -!- PTM: It is not established whether the 2,3-didehydrobutyrines are the
CC E- or Z-isomers (PubMed:15023056 and PubMed:10608807). In the NMR model
CC they were assumed to be the Z-isomer.
CC -!- MASS SPECTROMETRY: Mass=2848; Method=Plasma desorption;
CC Evidence={ECO:0000269|PubMed:10971756};
CC -!- MASS SPECTROMETRY: Mass=2847.40; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15023056};
CC -!- MASS SPECTROMETRY: Mass=2847.47; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10608807};
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DR EMBL; AE001272; AAC56054.1; -; Genomic_DNA.
DR EMBL; AF167432; AAF32257.1; -; Genomic_DNA.
DR PIR; T43107; T43107.
DR RefSeq; NP_047320.1; NC_001949.1.
DR AlphaFoldDB; O87237; -.
DR TCDB; 1.C.21.2.4; the lacticin 481 (lacticin 481) family.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW Lantibiotic; Plasmid; Secreted; Thioether bond.
FT PROPEP 1..36
FT /evidence="ECO:0000269|PubMed:15023056"
FT /id="PRO_0000042851"
FT PEPTIDE 37..65
FT /note="Lantibiotic lacticin 3147 A2"
FT /evidence="ECO:0000269|PubMed:15023056"
FT /id="PRO_0000042852"
FT MOD_RES 37
FT /note="2-oxobutanoic acid"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 38
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 41
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 45
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT MOD_RES 48
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 52..56
FT /note="Lanthionine (Ser-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 58..61
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
FT CROSSLNK 62..65
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:15023056"
SQ SEQUENCE 65 AA; 7080 MW; DD063A6B5DCE5F82 CRC64;
MKEKNMKKND TIELQLGKYL EDDMIELAEG DESHGGTTPA TPAISILSAY ISTNTCPTTK
CTRAC
