LANA_RUMGN
ID LANA_RUMGN Reviewed; 47 AA.
AC P83674; P83676; P83677; Q8VLK0; Q9K381;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Ruminococcin-A;
DE AltName: Full=RumA protein 1/2/3;
DE Flags: Precursor;
GN Name=rumA1;
GN and
GN Name=rumA2;
GN and
GN Name=rumA3;
OS Ruminococcus gnavus.
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae;
OC Mediterraneibacter.
OX NCBI_TaxID=33038 {ECO:0000312|EMBL:CAB93673.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), PROTEIN
RP SEQUENCE OF 24-44, DEHYDRATION AT THR-30 AND THR-39, LANTHIONINE
RP CROSS-LINKS, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC STRAIN=E1 {ECO:0000312|EMBL:CAB93673.1};
RX PubMed=11526013; DOI=10.1128/aem.67.9.4111-4118.2001;
RA Dabard J., Bridonneau C., Phillipe C., Anglade P., Molle D., Nardi M.,
RA Ladire M., Girardin H., Marcille F., Gomez A., Fons M.;
RT "Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain
RT isolated from human feces.";
RL Appl. Environ. Microbiol. 67:4111-4118(2001).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3).
RC STRAIN=LEMB04 {ECO:0000312|EMBL:AAL73933.1},
RC LEMB53 {ECO:0000312|EMBL:AAL73945.1}, LEMV58 {ECO:0000312|EMBL:AAL73927.1},
RC LEMV66 {ECO:0000312|EMBL:AAL73924.1}, LEMV95 {ECO:0000312|EMBL:AAL73930.1},
RC and LEMV99 {ECO:0000312|EMBL:AAL73939.1};
RX PubMed=12089024; DOI=10.1128/aem.68.7.3424-3431.2002;
RA Marcille F., Gomez A., Joubert P., Ladire M., Veau G., Clara A., Gavini F.,
RA Willems A., Fons M.;
RT "Distribution of genes encoding the trypsin-dependent lantibiotic
RT ruminococcin A among bacteria isolated from human fecal microbiota.";
RL Appl. Environ. Microbiol. 68:3424-3431(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RUMA1; RUMA2 AND RUMA3), DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC STRAIN=E1 {ECO:0000312|EMBL:AAL73945.1};
RX PubMed=11741840; DOI=10.1128/jb.184.1.18-28.2002;
RA Gomez A., Ladire M., Marcille F., Fons M.;
RT "Trypsin mediates growth phase-dependent transcriptional regulation of
RT genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by
RT a Ruminococcus gnavus strain from a human intestinal microbiota.";
RL J. Bacteriol. 184:18-28(2002).
CC -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC active on Gram-positive bacteria. The bactericidal activity of
CC lantibiotics is based on depolarization of energized bacterial
CC cytoplasmic membranes, initiated by the formation of aqueous
CC transmembrane pores. Ruminococcin A is a broad spectrum bacteriocin
CC exhibiting activity against a wide range of pathogenic clostridia and
CC B.longum. {ECO:0000250|UniProtKB:P36499, ECO:0000269|PubMed:11526013}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11526013}.
CC -!- DEVELOPMENTAL STAGE: Highest expression in mid to late logarithmic
CC growth phase, after which expression decreases slowly before leveling
CC off in the stationary growth phase. {ECO:0000269|PubMed:11741840}.
CC -!- INDUCTION: By trypsin. {ECO:0000269|PubMed:11741840}.
CC -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC with cysteine. This is followed by membrane translocation and cleavage
CC of the modified precursor. {ECO:0000303|PubMed:11526013}.
CC -!- PTM: It is not established whether the 2,3-didehydrobutyrine is the
CC E- or Z-isomer. {ECO:0000269|PubMed:11526013}.
CC -!- MASS SPECTROMETRY: Mass=2675.17; Mass_error=0.14; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:11526013};
CC -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000305}.
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DR EMBL; AF320327; AAK73193.1; -; Genomic_DNA.
DR EMBL; AF320327; AAK73194.1; -; Genomic_DNA.
DR EMBL; AF320327; AAK73195.1; -; Genomic_DNA.
DR EMBL; AF439548; AAL73922.1; -; Genomic_DNA.
DR EMBL; AF439548; AAL73923.1; -; Genomic_DNA.
DR EMBL; AF439548; AAL73924.1; -; Genomic_DNA.
DR EMBL; AF439549; AAL73925.1; -; Genomic_DNA.
DR EMBL; AF439549; AAL73926.1; -; Genomic_DNA.
DR EMBL; AF439549; AAL73927.1; -; Genomic_DNA.
DR EMBL; AF439550; AAL73928.1; -; Genomic_DNA.
DR EMBL; AF439550; AAL73929.1; -; Genomic_DNA.
DR EMBL; AF439550; AAL73930.1; -; Genomic_DNA.
DR EMBL; AF439551; AAL73931.1; -; Genomic_DNA.
DR EMBL; AF439551; AAL73932.1; -; Genomic_DNA.
DR EMBL; AF439551; AAL73933.1; -; Genomic_DNA.
DR EMBL; AF439553; AAL73937.1; -; Genomic_DNA.
DR EMBL; AF439553; AAL73938.1; -; Genomic_DNA.
DR EMBL; AF439553; AAL73939.1; -; Genomic_DNA.
DR EMBL; AF439554; AAL73943.1; -; Genomic_DNA.
DR EMBL; AF439554; AAL73944.1; -; Genomic_DNA.
DR EMBL; AF439554; AAL73945.1; -; Genomic_DNA.
DR EMBL; AJ276653; CAB93671.1; -; Genomic_DNA.
DR EMBL; AJ276653; CAB93672.1; -; Genomic_DNA.
DR EMBL; AJ276653; CAB93673.1; -; Genomic_DNA.
DR RefSeq; WP_071144043.1; NZ_JADMTD010000012.1.
DR AlphaFoldDB; P83674; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0006965; P:positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria; IDA:UniProtKB.
DR InterPro; IPR007682; Lantibiotic_typ-A_Lactobact.
DR Pfam; PF04604; L_biotic_typeA; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW Lantibiotic; Secreted; Signal; Thioether bond.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:11526013"
FT PEPTIDE 24..47
FT /note="Ruminococcin-A"
FT /id="PRO_0000017127"
FT MOD_RES 30
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000305|PubMed:11526013"
FT MOD_RES 39
FT /note="2,3-didehydrobutyrine"
FT /evidence="ECO:0000305|PubMed:11526013"
FT CROSSLNK 30..35
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000305|PubMed:11526013"
FT CROSSLNK 32..46
FT /note="Lanthionine (Ser-Cys)"
FT /evidence="ECO:0000305|PubMed:11526013"
FT CROSSLNK 45..47
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000305|PubMed:11526013"
FT VARIANT 15
FT /note="K -> N (in strain: LEMB04 and LEMV99)"
FT /evidence="ECO:0000269|PubMed:12089024"
SQ SEQUENCE 47 AA; 5360 MW; 2D70514404D1FED9 CRC64;
MRNDVLTLTN PMEEKELEQI LGGGNGVLKT ISHECNMNTW QFLFTCC
