ID   LANA_STRMD              Reviewed;          58 AA.
AC   H2A7G5;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Lantibiotic macedovicin {ECO:0000303|PubMed:23122510};
DE   Flags: Precursor;
GN   OrderedLocusNames=SMA_1409 {ECO:0000312|EMBL:CCF02700.1};
OS   Streptococcus macedonicus (strain ACA-DC 198).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1116231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ACA-DC 198;
RX   PubMed=22408241; DOI=10.1128/jb.06804-11;
RA   Papadimitriou K., Ferreira S., Papandreou N.C., Mavrogonatou E., Supply P.,
RA   Pot B., Tsakalidou E.;
RT   "Complete genome sequence of the dairy isolate Streptococcus macedonicus
RT   ACA-DC 198.";
RL   J. Bacteriol. 194:1838-1839(2012).
RN   [2]
RP   PROTEIN SEQUENCE OF 26-45, FUNCTION, MASS SPECTROMETRY, DEHYDRATION AT
RP   THR-33 AND THR-35, AND LANTHIONINE CROSS-LINKS.
RC   STRAIN=ACA-DC 198;
RX   PubMed=23122510; DOI=10.1016/j.fm.2012.09.008;
RA   Georgalaki M., Papadimitriou K., Anastasiou R., Pot B., Van Driessche G.,
RA   Devreese B., Tsakalidou E.;
RT   "Macedovicin, the second food-grade lantibiotic produced by Streptococcus
RT   macedonicus ACA-DC 198.";
RL   Food Microbiol. 33:124-130(2013).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC       active on Gram-positive bacteria. Macedovicin inhibits a broad spectrum
CC       of lactic acid bacteria, several food spoilage species (e.g.
CC       Clostridium spp.) and oral streptococci (PubMed:23122510). The
CC       bactericidal activity of lantibiotics is based on depolarization of
CC       energized bacterial cytoplasmic membranes, initiated by the formation
CC       of aqueous transmembrane pores. {ECO:0000269|PubMed:23122510,
CC       ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:23122510}.
CC   -!- PTM: Maturation of macedovicin involves the enzymatic dehydration of
CC       Thr-33 and Thr-35 into dehydrobutyrine residues, that can form a beta-
CC       methyllanthionine bond with Cys-38 and Cys-57, respectively
CC       (PubMed:23122510). This is followed by membrane translocation and
CC       cleavage of the modified precursor. {ECO:0000269|PubMed:23122510,
CC       ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3428.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23122510};
CC   -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000305}.
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DR   EMBL; HE613569; CCF02700.1; -; Genomic_DNA.
DR   AlphaFoldDB; H2A7G5; -.
DR   SMR; H2A7G5; -.
DR   STRING; 1116231.SMA_1409; -.
DR   EnsemblBacteria; CCF02700; CCF02700; SMA_1409.
DR   KEGG; smn:SMA_1409; -.
DR   HOGENOM; CLU_3022031_0_0_9; -.
DR   OMA; KTLTKDC; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR007682; Lantibiotic_typ-A_Lactobact.
DR   Pfam; PF04604; L_biotic_typeA; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Disulfide bond; Lantibiotic; Secreted; Thioether bond.
FT   PROPEP          1..25
FT                   /evidence="ECO:0000269|PubMed:23122510"
FT                   /id="PRO_0000443307"
FT   CHAIN           26..58
FT                   /note="Lantibiotic macedovicin"
FT                   /id="PRO_0000443308"
FT   MOD_RES         33
FT                   /note="2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:23122510"
FT   MOD_RES         35
FT                   /note="2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:23122510"
FT   DISULFID        46..54
FT                   /evidence="ECO:0000269|PubMed:23122510"
FT   CROSSLNK        33..38
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:23122510"
FT   CROSSLNK        35..57
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:23122510"
SQ   SEQUENCE   58 AA;  6250 MW;  8E3555B7069F6C43 CRC64;
     MMNATENQIF VETVSDQELE MLIGGADRGW IKTLTKDCPN VISSICAGTI ITACKNCA