ID   LANE_STAEP              Reviewed;          52 AA.
AC   P08136; Q54093;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Lantibiotic epidermin;
DE   Flags: Precursor;
GN   Name=epiA;
OS   Staphylococcus epidermidis.
OG   Plasmid pTu 32.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TU 3298 / DSM 3095;
RX   PubMed=2835685; DOI=10.1038/333276a0;
RA   Schnell N., Entian K.-D., Schneider U., Gotz F., Zahner H., Kellner R.,
RA   Jung G.;
RT   "Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic
RT   with four sulphide-rings.";
RL   Nature 333:276-278(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TU 3298 / DSM 3095;
RX   PubMed=1740156; DOI=10.1111/j.1432-1033.1992.tb16605.x;
RA   Schnell N., Engelke G., Augustin J., Rosenstein R., Ungermann V., Goetz F.,
RA   Entian K.-D.;
RT   "Analysis of genes involved in the biosynthesis of lantibiotic epidermin.";
RL   Eur. J. Biochem. 204:57-68(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 31-52, DEHYDRATION AT THR-44, AND LANTHIONINE
RP   CROSS-LINKS.
RX   PubMed=3769923; DOI=10.1111/j.1432-1033.1986.tb09933.x;
RA   Allgaier H., Jung G., Werner R.G., Schneider U., Zahner H.;
RT   "Epidermin: sequencing of a heterodetic tetracyclic 21-peptide amide
RT   antibiotic.";
RL   Eur. J. Biochem. 160:9-22(1986).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.57 ANGSTROMS) OF 48-52 IN COMPLEX WITH EPID.
RX   PubMed=11101502; DOI=10.1093/emboj/19.23.6299;
RA   Blaesse M., Kupke T., Huber R., Steinbacher S.;
RT   "Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed
RT   with a pentapeptide substrate.";
RL   EMBO J. 19:6299-6310(2000).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC       active on Gram-positive bacteria. The bactericidal activity of
CC       lantibiotics is based on depolarization of energized bacterial
CC       cytoplasmic membranes, initiated by the formation of aqueous
CC       transmembrane pores.
CC   -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC       Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC       with cysteine. The C-terminal lanthionine undergoes decarboxylation.
CC       This is followed by membrane translocation and cleavage of the modified
CC       precursor.
CC   -!- PTM: The 2,3-didehydrobutyrine is determined to be the Z-isomer.
CC       {ECO:0000269|PubMed:3769923}.
CC   -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000305}.
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DR   EMBL; X07840; CAA30689.1; -; Genomic_DNA.
DR   EMBL; X07840; CAA30690.1; -; Genomic_DNA.
DR   EMBL; X62386; CAA44252.1; -; Genomic_DNA.
DR   PIR; S00768; EPSED.
DR   RefSeq; WP_002498697.1; NZ_CZRO020000143.1.
DR   PDB; 1G5Q; X-ray; 2.57 A; M/N/O/P=48-52.
DR   PDBsum; 1G5Q; -.
DR   AlphaFoldDB; P08136; -.
DR   SMR; P08136; -.
DR   TCDB; 1.C.20.1.6; the nisin (nisin) family.
DR   EvolutionaryTrace; P08136; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR006079; Lantibiotic_typ-A_Bacillales.
DR   Pfam; PF02052; Gallidermin; 1.
DR   PRINTS; PR00323; GALLIDERMIN.
DR   TIGRFAMs; TIGR03731; lantibio_gallid; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin; D-amino acid;
KW   Direct protein sequencing; Lantibiotic; Plasmid; Thioether bond.
FT   PROPEP          1..30
FT                   /evidence="ECO:0000269|PubMed:3769923"
FT                   /id="PRO_0000017116"
FT   PEPTIDE         31..52
FT                   /note="Lantibiotic epidermin"
FT                   /evidence="ECO:0000269|PubMed:2835685"
FT                   /id="PRO_0000017117"
FT   MOD_RES         44
FT                   /note="(Z)-2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:3769923"
FT   CROSSLNK        33..37
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:3769923"
FT   CROSSLNK        38..41
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000269|PubMed:3769923"
FT   CROSSLNK        46..51
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:3769923"
FT   CROSSLNK        49..52
FT                   /note="S-(2-aminovinyl)-D-cysteine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:3769923"
SQ   SEQUENCE   52 AA;  5632 MW;  8B1AD2875BF16D6D CRC64;
     MEAVKEKNDL FNLDVKVNAK ESNDSGAEPR IASKFICTPG CAKTGSFNSY CC