ID   LANNA_STAHO             Reviewed;          57 AA.
AC   B5MFD0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Lantibiotic nukacin {ECO:0000250|UniProtKB:Q9KWM4};
DE   AltName: Full=Nukacin KQ-1;
DE   AltName: Full=Nukacin KQU-131 {ECO:0000303|PubMed:18685189};
DE   Flags: Precursor;
GN   Name=nukA {ECO:0000250|UniProtKB:Q9KWM4};
GN   Synonyms=nkqA {ECO:0000312|EMBL:BAG70955.1};
OS   Staphylococcus hominis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1290;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAG70955.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-38,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND MASS SPECTROMETRY.
RC   STRAIN=KQU-131 {ECO:0000269|PubMed:18685189};
RX   PubMed=18685189; DOI=10.1271/bbb.80239;
RA   Wilaipun P., Zendo T., Okuda K., Nakayama J., Sonomoto K.;
RT   "Identification of the nukacin KQU-131, a new type-A(II) lantibiotic
RT   produced by Staphylococcus hominis KQU-131 isolated from Thai fermented
RT   fish product (Pla-ra).";
RL   Biosci. Biotechnol. Biochem. 72:2232-2235(2008).
CC   -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC       active on Gram-positive bacteria. The bactericidal activity of
CC       lantibiotics is based on depolarization of energized bacterial
CC       cytoplasmic membranes, initiated by the formation of aqueous
CC       transmembrane pores (By similarity). {ECO:0000250|UniProtKB:Q9KWM4}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Retains 50% of its antimicrobial activity after heating at 100
CC         degrees Celsius for 100 minutes, and retains 25% antimicrobial
CC         activity after heating at 121 Celsius for 15 minutes.
CC         {ECO:0000269|PubMed:18685189};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9KWM4}.
CC       Note=Through the specific ABC transporter nukT.
CC       {ECO:0000250|UniProtKB:Q9KWM4}.
CC   -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC       Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC       with cysteine. This is followed by membrane translocation and cleavage
CC       of the modified precursor (By similarity).
CC       {ECO:0000250|UniProtKB:Q9KWM4}.
CC   -!- MASS SPECTROMETRY: Mass=3003.97; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18685189};
CC   -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000255}.
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DR   EMBL; AB432987; BAG70955.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5MFD0; -.
DR   TCDB; 1.C.21.1.8; the lacticin 481 (lacticin 481) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR007682; Lantibiotic_typ-A_Lactobact.
DR   Pfam; PF04604; L_biotic_typeA; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Lantibiotic; Secreted; Thioether bond.
FT   PROPEP          1..30
FT                   /evidence="ECO:0000269|PubMed:18685189"
FT                   /id="PRO_0000408769"
FT   PEPTIDE         31..57
FT                   /note="Lantibiotic nukacin"
FT                   /evidence="ECO:0000269|PubMed:18685189"
FT                   /id="PRO_0000408770"
FT   MOD_RES         54
FT                   /note="2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9KWM4"
FT   CROSSLNK        39..44
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9KWM4"
FT   CROSSLNK        41..55
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9KWM4"
FT   CROSSLNK        48..56
FT                   /note="Beta-methyllanthionine (Thr-Cys)"
FT                   /evidence="ECO:0000250|UniProtKB:Q9KWM4"
SQ   SEQUENCE   57 AA;  6463 MW;  9BF6DF7549E0A5F6 CRC64;
     MENSKIMKDI EVANLLEEVQ EDELNEVLGA KKKSGVIPTV SHDCHMNTFQ FMFTCCS