LANN_LACLL
ID LANN_LACLL Reviewed; 57 AA.
AC P13068;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Lantibiotic nisin-A;
DE Flags: Precursor;
GN Name=spaN; Synonyms=nisA;
OS Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG Plasmid unnamed.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1360;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 11454 / DSM 20729 / LMG 7930 / NCDO 496 / NCIMB 8586 / Berridge
RC X 13;
RX PubMed=3141403; DOI=10.1016/s0021-9258(18)37587-2;
RA Buchman G.W., Banerjee S., Hansen J.N.;
RT "Structure, expression, and evolution of a gene encoding the precursor of
RT nisin, a small protein antibiotic.";
RL J. Biol. Chem. 263:16260-16266(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 11454 / DSM 20729 / LMG 7930 / NCDO 496 / NCIMB 8586 / Berridge
RC X 13;
RX PubMed=1905517; DOI=10.1128/aem.57.4.1181-1188.1991;
RA Steen M.T., Chung Y.J., Hansen J.N.;
RT "Characterization of the nisin gene as part of a polycistronic operon in
RT the chromosome of Lactococcus lactis ATCC 11454.";
RL Appl. Environ. Microbiol. 57:1181-1188(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=6F3; PLASMID=unnamed;
RX PubMed=2493449; DOI=10.1128/jb.171.3.1597-1601.1989;
RA Kaletta C., Entian K.-D.;
RT "Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and
RT posttranslational processing of its peptide product.";
RL J. Bacteriol. 171:1597-1601(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=6F3;
RX PubMed=1482192; DOI=10.1128/aem.58.11.3730-3743.1992;
RA Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D.;
RT "Biosynthesis of the lantibiotic nisin: genomic organization and membrane
RT localization of the NisB protein.";
RL Appl. Environ. Microbiol. 58:3730-3743(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NIZO R5; TRANSPOSON=Tn5276;
RX PubMed=7689965; DOI=10.1111/j.1432-1033.1993.tb18143.x;
RA Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.;
RT "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus
RT lactis. Requirement of expression of the nisA and nisI genes for
RT development of immunity.";
RL Eur. J. Biochem. 216:281-291(1993).
RN [6]
RP PROTEIN SEQUENCE OF 24-57.
RA Gross E.;
RL (In) Friedman M. (eds.);
RL Protein cross-linking, pp.131-153, Plenum Press, New York (1977).
RN [7]
RP PROTEIN SEQUENCE OF 24-57, DEHYDRATION AT THR-25; SER-28 AND SER-56, AND
RP LANTHIONINE CROSS-LINKS.
RX PubMed=5131162; DOI=10.1021/ja00747a073;
RA Gross E., Morell J.L.;
RT "The structure of nisin.";
RL J. Am. Chem. Soc. 93:4634-4635(1971).
RN [8]
RP STRUCTURE BY NMR.
RX PubMed=1765078; DOI=10.1111/j.1432-1033.1991.tb16488.x;
RA van de Ven F.J., van den Hooven H.W., Konings R.N.H., Hilbers C.W.;
RT "NMR studies of lantibiotics. The structure of nisin in aqueous solution.";
RL Eur. J. Biochem. 202:1181-1188(1991).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=1575686; DOI=10.1042/bj2830413;
RA Lian L.-Y., Chan W.C., Morley S.D., Roberts G.C.K., Bycroft B.W.,
RA Jackson D.;
RT "Solution structures of nisin A and its two major degradation products
RT determined by NMR.";
RL Biochem. J. 283:413-420(1992).
RN [10]
RP STRUCTURE BY NMR.
RX PubMed=8454055; DOI=10.1016/0014-5793(93)80065-3;
RA van den Hooven H.W., Fogolari F., Rollema H.S., Konings R.N.H.,
RA Hilbers C.W., van de Ven F.J.;
RT "NMR and circular dichroism studies of the lantibiotic nisin in non-aqueous
RT environments.";
RL FEBS Lett. 319:189-194(1993).
RN [11]
RP STRUCTURE BY NMR.
RX PubMed=8418850; DOI=10.1021/bi00052a039;
RA Sailer M., Helms G.L., Henkel T., Niemczura W.P., Stiles M.E.,
RA Vederas J.C.;
RT "15N- and 13C-labeled media from Anabaena sp. for universal isotopic
RT labeling of bacteriocins: NMR resonance assignments of leucocin A from
RT Leuconostoc gelidum and nisin A from Lactococcus lactis.";
RL Biochemistry 32:310-318(1993).
CC -!- FUNCTION: Lanthionine-containing peptide antibiotic (lantibiotic)
CC active on Gram-positive bacteria. The bactericidal activity of
CC lantibiotics is based on depolarization of energized bacterial
CC cytoplasmic membranes, initiated by the formation of aqueous
CC transmembrane pores.
CC -!- PTM: Maturation of lantibiotics involves the enzymatic conversion of
CC Thr, and Ser into dehydrated AA and the formation of thioether bonds
CC with cysteine. This is followed by membrane translocation and cleavage
CC of the modified precursor.
CC -!- PTM: The structure of the 2,3-didehydrobutyrine is not discussed in
CC PubMed:8454055. However, in Fig. 1 the residue is diagrammed as the Z-
CC isomer.
CC -!- BIOTECHNOLOGY: Used as a food preservative.
CC -!- MISCELLANEOUS: In strain 6F3 the nisA gene is encoded on an unnamed
CC plasmid. The reported protein sequences are identical.
CC {ECO:0000303|PubMed:2493449}.
CC -!- SIMILARITY: Belongs to the type A lantibiotic family. {ECO:0000305}.
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DR EMBL; J04057; AAA88606.1; -; Genomic_DNA.
DR EMBL; M65089; AAA73038.1; -; Genomic_DNA.
DR EMBL; M24527; AAA26948.1; -; Genomic_DNA.
DR EMBL; X68307; CAA48380.1; -; Genomic_DNA.
DR EMBL; M27277; AAA25188.1; -; Genomic_DNA.
DR EMBL; D00696; BAA00602.1; -; Genomic_DNA.
DR EMBL; L16226; AAA25189.1; -; Genomic_DNA.
DR EMBL; M79445; AAA25198.1; -; Genomic_DNA.
DR PIR; A31915; NILLSA.
DR RefSeq; WP_014570405.1; NZ_ML956318.1.
DR AlphaFoldDB; P13068; -.
DR SMR; P13068; -.
DR TCDB; 1.C.20.1.1; the nisin (nisin) family.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR InterPro; IPR006079; Lantibiotic_typ-A_Bacillales.
DR Pfam; PF02052; Gallidermin; 1.
DR PRINTS; PR00324; NISIN.
DR TIGRFAMs; TIGR03731; lantibio_gallid; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW Lantibiotic; Plasmid; Thioether bond.
FT PROPEP 1..23
FT /evidence="ECO:0000269|PubMed:5131162, ECO:0000269|Ref.6"
FT /id="PRO_0000017122"
FT PEPTIDE 24..57
FT /note="Lantibiotic nisin-A"
FT /id="PRO_0000017123"
FT MOD_RES 25
FT /note="(Z)-2,3-didehydrobutyrine"
FT /evidence="ECO:0000269|PubMed:5131162"
FT MOD_RES 28
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT MOD_RES 56
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT CROSSLNK 26..30
FT /note="Lanthionine (Ser-Cys)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT CROSSLNK 31..34
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT CROSSLNK 36..42
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT CROSSLNK 46..49
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:5131162"
FT CROSSLNK 48..51
FT /note="Beta-methyllanthionine (Thr-Cys)"
FT /evidence="ECO:0000269|PubMed:5131162"
SQ SEQUENCE 57 AA; 5963 MW; 315E4428AC70BFEA CRC64;
MSTKDFNLDL VSVSKKDSGA SPRITSISLC TPGCKTGALM GCNMKTATCH CSIHVSK
