LAP4A_HUMAN
ID LAP4A_HUMAN Reviewed; 233 AA.
AC Q15012; Q6UW22;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Lysosomal-associated transmembrane protein 4A;
DE AltName: Full=Golgi 4-transmembrane-spanning transporter MTP;
GN Name=LAPTM4A; Synonyms=KIAA0108, LAPTM4, MBNT, MTRP;
GN ORFNames=UNQ1846/PRO3574;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow;
RX PubMed=7788527; DOI=10.1093/dnares/2.1.37;
RA Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S.,
RA Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N.;
RT "Prediction of the coding sequences of unidentified human genes. III. The
RT coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of
RT cDNA clones from human cell line KG-1.";
RL DNA Res. 2:37-43(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [6]
RP ACETYLATION AT MET-1.
RX PubMed=25732826; DOI=10.1016/j.celrep.2015.01.053;
RA Aksnes H., Van Damme P., Goris M., Starheim K.K., Marie M., Stoeve S.I.,
RA Hoel C., Kalvik T.V., Hole K., Glomnes N., Furnes C., Ljostveit S.,
RA Ziegler M., Niere M., Gevaert K., Arnesen T.;
RT "An organellar nalpha-acetyltransferase, naa60, acetylates cytosolic N
RT termini of transmembrane proteins and maintains Golgi integrity.";
RL Cell Rep. 10:1362-1374(2015).
CC -!- FUNCTION: May function in the transport of nucleosides and/or
CC nucleoside derivatives between the cytosol and the lumen of an
CC intracellular membrane-bound compartment. {ECO:0000250}.
CC -!- INTERACTION:
CC Q15012; P46379-2: BAG6; NbExp=3; IntAct=EBI-723416, EBI-10988864;
CC Q15012; Q8WUW1: BRK1; NbExp=3; IntAct=EBI-723416, EBI-2837444;
CC Q15012; G5E9A7: DMWD; NbExp=3; IntAct=EBI-723416, EBI-10976677;
CC Q15012; P29692-2: EEF1D; NbExp=3; IntAct=EBI-723416, EBI-5280572;
CC Q15012; O15552: FFAR2; NbExp=3; IntAct=EBI-723416, EBI-2833872;
CC Q15012; O14901: KLF11; NbExp=3; IntAct=EBI-723416, EBI-948266;
CC Q15012; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-723416, EBI-5235340;
CC Q15012; Q6PL24: TMED8; NbExp=3; IntAct=EBI-723416, EBI-11603430;
CC Q15012; P14927: UQCRB; NbExp=3; IntAct=EBI-723416, EBI-743128;
CC -!- SUBCELLULAR LOCATION: Endomembrane system {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=May reside in an intracellular
CC membrane-bound compartment. {ECO:0000305}.
CC -!- DOMAIN: The C-terminal domain is necessary for retention within
CC intracellular membranes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LAPTM4/LAPTM5 transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ89387.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAA03522.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; D14696; BAA03522.2; ALT_INIT; mRNA.
DR EMBL; AY359028; AAQ89387.1; ALT_FRAME; mRNA.
DR EMBL; BC000421; AAH00421.1; -; mRNA.
DR CCDS; CCDS1696.1; -.
DR RefSeq; NP_055528.1; NM_014713.4.
DR AlphaFoldDB; Q15012; -.
DR BioGRID; 115089; 33.
DR IntAct; Q15012; 22.
DR MINT; Q15012; -.
DR STRING; 9606.ENSP00000175091; -.
DR iPTMnet; Q15012; -.
DR PhosphoSitePlus; Q15012; -.
DR SwissPalm; Q15012; -.
DR BioMuta; LAPTM4A; -.
DR DMDM; 3122413; -.
DR EPD; Q15012; -.
DR jPOST; Q15012; -.
DR MassIVE; Q15012; -.
DR PaxDb; Q15012; -.
DR PeptideAtlas; Q15012; -.
DR PRIDE; Q15012; -.
DR ProteomicsDB; 60364; -.
DR Antibodypedia; 27203; 134 antibodies from 21 providers.
DR DNASU; 9741; -.
DR Ensembl; ENST00000175091.5; ENSP00000175091.4; ENSG00000068697.7.
DR GeneID; 9741; -.
DR KEGG; hsa:9741; -.
DR MANE-Select; ENST00000175091.5; ENSP00000175091.4; NM_014713.5; NP_055528.1.
DR CTD; 9741; -.
DR DisGeNET; 9741; -.
DR GeneCards; LAPTM4A; -.
DR HGNC; HGNC:6924; LAPTM4A.
DR HPA; ENSG00000068697; Low tissue specificity.
DR MIM; 618837; gene.
DR neXtProt; NX_Q15012; -.
DR OpenTargets; ENSG00000068697; -.
DR PharmGKB; PA30295; -.
DR VEuPathDB; HostDB:ENSG00000068697; -.
DR eggNOG; ENOG502QSAX; Eukaryota.
DR GeneTree; ENSGT00940000153446; -.
DR HOGENOM; CLU_059239_2_0_1; -.
DR InParanoid; Q15012; -.
DR OMA; HPNTVPT; -.
DR OrthoDB; 1145926at2759; -.
DR PhylomeDB; Q15012; -.
DR TreeFam; TF330843; -.
DR PathwayCommons; Q15012; -.
DR SignaLink; Q15012; -.
DR BioGRID-ORCS; 9741; 16 hits in 1081 CRISPR screens.
DR ChiTaRS; LAPTM4A; human.
DR GenomeRNAi; 9741; -.
DR Pharos; Q15012; Tbio.
DR PRO; PR:Q15012; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q15012; protein.
DR Bgee; ENSG00000068697; Expressed in seminal vesicle and 218 other tissues.
DR ExpressionAtlas; Q15012; baseline and differential.
DR Genevisible; Q15012; HS.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IDA:UniProtKB.
DR GO; GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
DR InterPro; IPR004687; LAPTM4/5.
DR InterPro; IPR018396; LAPTM_4A/5.
DR Pfam; PF03821; Mtp; 2.
DR TIGRFAMs; TIGR00799; mtp; 1.
PE 1: Evidence at protein level;
KW Acetylation; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..233
FT /note="Lysosomal-associated transmembrane protein 4A"
FT /id="PRO_0000096637"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:25732826,
FT ECO:0007744|PubMed:22814378"
SQ SEQUENCE 233 AA; 26801 MW; 9BB76152B6E1365E CRC64;
MVSMSFKRNR SDRFYSTRCC GCCHVRTGTI ILGTWYMVVN LLMAILLTVE VTHPNSMPAV
NIQYEVIGNY YSSERMADNA CVLFAVSVLM FIISSMLVYG AISYQVGWLI PFFCYRLFDF
VLSCLVAISS LTYLPRIKEY LDQLPDFPYK DDLLALDSSC LLFIVLVFFA LFIIFKAYLI
NCVWNCYKYI NNRNVPEIAV YPAFEAPPQY VLPTYEMAVK MPEKEPPPPY LPA
