LAPA_ECOLI
ID LAPA_ECOLI Reviewed; 102 AA.
AC P0ACV4; P77614;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Lipopolysaccharide assembly protein A {ECO:0000255|HAMAP-Rule:MF_01948, ECO:0000303|PubMed:24722986};
GN Name=lapA {ECO:0000255|HAMAP-Rule:MF_01948, ECO:0000303|PubMed:24722986};
GN Synonyms=yciS; OrderedLocusNames=b1279, JW1271;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24722986; DOI=10.1074/jbc.m113.539494;
RA Klein G., Kobylak N., Lindner B., Stupak A., Raina S.;
RT "Assembly of lipopolysaccharide in Escherichia coli requires the essential
RT LapB heat shock protein.";
RL J. Biol. Chem. 289:14829-14853(2014).
CC -!- FUNCTION: Involved in the assembly of lipopolysaccharide (LPS).
CC {ECO:0000255|HAMAP-Rule:MF_01948, ECO:0000269|PubMed:24722986}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01948, ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:24722986}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01948}. Note=Copurifies with LptE/LptD,
CC LptBFGC, LptA, DnaK/DnaJ and LPS. {ECO:0000269|PubMed:24722986}.
CC -!- INDUCTION: Induced by heat shock, via RpoH.
CC {ECO:0000269|PubMed:24722986}.
CC -!- DISRUPTION PHENOTYPE: Disruption mutant shows accumulation of a few
CC incomplete LPS precursors. {ECO:0000269|PubMed:24722986}.
CC -!- SIMILARITY: Belongs to the LapA family. {ECO:0000255|HAMAP-
CC Rule:MF_01948, ECO:0000305}.
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DR EMBL; U00096; AAC74361.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA14833.1; -; Genomic_DNA.
DR PIR; B64876; B64876.
DR RefSeq; NP_415795.1; NC_000913.3.
DR RefSeq; WP_000876286.1; NZ_STEB01000005.1.
DR AlphaFoldDB; P0ACV4; -.
DR BioGRID; 4260130; 32.
DR DIP; DIP-47880N; -.
DR IntAct; P0ACV4; 20.
DR STRING; 511145.b1279; -.
DR jPOST; P0ACV4; -.
DR PaxDb; P0ACV4; -.
DR PRIDE; P0ACV4; -.
DR EnsemblBacteria; AAC74361; AAC74361; b1279.
DR EnsemblBacteria; BAA14833; BAA14833; BAA14833.
DR GeneID; 66674896; -.
DR GeneID; 944936; -.
DR KEGG; ecj:JW1271; -.
DR KEGG; eco:b1279; -.
DR PATRIC; fig|1411691.4.peg.1002; -.
DR EchoBASE; EB3663; -.
DR eggNOG; COG3771; Bacteria.
DR HOGENOM; CLU_160072_0_0_6; -.
DR OMA; QGDYRIS; -.
DR PhylomeDB; P0ACV4; -.
DR BioCyc; EcoCyc:G6637-MON; -.
DR PRO; PR:P0ACV4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0008653; P:lipopolysaccharide metabolic process; IEA:InterPro.
DR HAMAP; MF_01948; LPS_assembly_LapA; 1.
DR InterPro; IPR032906; LapA.
DR InterPro; IPR010445; LapA_dom.
DR Pfam; PF06305; LapA_dom; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Stress response; Transmembrane; Transmembrane helix.
FT CHAIN 1..102
FT /note="Lipopolysaccharide assembly protein A"
FT /id="PRO_0000168886"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01948"
FT TOPO_DOM 24..43
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01948"
FT TOPO_DOM 65..102
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
FT COILED 64..92
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01948"
SQ SEQUENCE 102 AA; 11351 MW; 716C2881E2E2EDFC CRC64;
MKYLLIFLLV LAIFVISVTL GAQNDQQVTF NYLLAQGEYR ISTLLAVLFA AGFAIGWLIC
GLFWLRVRVS LARAERKIKR LENQLSPATD VAVVPHSSAA KE
