LAR4_DROME
ID LAR4_DROME Reviewed; 1531 AA.
AC Q9I7T7; A0A0H4YFU3; Q7KV72; Q7YTX9; Q9VZS2;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=La-related protein Larp4B {ECO:0000303|PubMed:29462618};
DE AltName: Full=La ribonucleoprotein domain family member Larp4B {ECO:0000305};
GN Name=Larp4B {ECO:0000303|PubMed:29462618, ECO:0000312|FlyBase:FBgn0035424};
GN ORFNames=CG11505 {ECO:0000303|PubMed:29462618};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.,
RA Booth B.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1123; SER-1370 AND SER-1413,
RP AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1370, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DOMAIN.
RX PubMed=29462618; DOI=10.1016/j.bbrc.2018.02.148;
RA Funakoshi M., Tsuda M., Muramatsu K., Hatsuda H., Morishita S., Aigaki T.;
RT "Overexpression of Larp4B downregulates dMyc and reduces cell and organ
RT sizes in Drosophila.";
RL Biochem. Biophys. Res. Commun. 497:762-768(2018).
CC -!- FUNCTION: Probable RNA binding protein. Negatively regulates myc at the
CC protein level, via an unknown mechanism, and may therefore have a role
CC in growth. Has no effect on myc mRNA levels.
CC {ECO:0000269|PubMed:29462618}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q9I7T7-1; Sequence=Displayed;
CC Name=A; Synonyms=D;
CC IsoId=Q9I7T7-2; Sequence=VSP_037217;
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development and in adults.
CC Ubiquitously expressed in the central nervous system and imaginal
CC disks. {ECO:0000269|PubMed:29462618}.
CC -!- DOMAIN: The HTH La-type RNA-binding and RRM domains are required for
CC its function in negatively regulating organ and cell size.
CC {ECO:0000269|PubMed:29462618}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ22587.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF47745.1; -; Genomic_DNA.
DR EMBL; AE014296; AAG22237.2; -; Genomic_DNA.
DR EMBL; AE014296; AAS64955.2; -; Genomic_DNA.
DR EMBL; BT010118; AAQ22587.1; ALT_SEQ; mRNA.
DR EMBL; BT150483; AKR06211.1; -; mRNA.
DR RefSeq; NP_647793.1; NM_139536.4. [Q9I7T7-1]
DR RefSeq; NP_728843.1; NM_168007.2. [Q9I7T7-2]
DR RefSeq; NP_995985.2; NM_206263.4. [Q9I7T7-2]
DR AlphaFoldDB; Q9I7T7; -.
DR SMR; Q9I7T7; -.
DR BioGRID; 63895; 62.
DR IntAct; Q9I7T7; 2.
DR STRING; 7227.FBpp0072910; -.
DR iPTMnet; Q9I7T7; -.
DR PaxDb; Q9I7T7; -.
DR PRIDE; Q9I7T7; -.
DR EnsemblMetazoa; FBtr0073045; FBpp0072909; FBgn0035424. [Q9I7T7-2]
DR EnsemblMetazoa; FBtr0073046; FBpp0072910; FBgn0035424. [Q9I7T7-1]
DR EnsemblMetazoa; FBtr0308061; FBpp0300406; FBgn0035424. [Q9I7T7-2]
DR GeneID; 38399; -.
DR KEGG; dme:Dmel_CG11505; -.
DR UCSC; CG11505-RA; d. melanogaster.
DR UCSC; CG11505-RB; d. melanogaster. [Q9I7T7-1]
DR UCSC; CG11505-RC; d. melanogaster.
DR CTD; 23185; -.
DR FlyBase; FBgn0035424; Larp4B.
DR VEuPathDB; VectorBase:FBgn0035424; -.
DR eggNOG; KOG2591; Eukaryota.
DR GeneTree; ENSGT00940000172042; -.
DR HOGENOM; CLU_247266_0_0_1; -.
DR InParanoid; Q9I7T7; -.
DR OMA; LAGHNEY; -.
DR OrthoDB; 771508at2759; -.
DR PhylomeDB; Q9I7T7; -.
DR SignaLink; Q9I7T7; -.
DR BioGRID-ORCS; 38399; 1 hit in 3 CRISPR screens.
DR ChiTaRS; CG11505; fly.
DR GenomeRNAi; 38399; -.
DR PRO; PR:Q9I7T7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035424; Expressed in brain and 16 other tissues.
DR ExpressionAtlas; Q9I7T7; baseline and differential.
DR Genevisible; Q9I7T7; DM.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISS:FlyBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:FlyBase.
DR GO; GO:0017148; P:negative regulation of translation; IMP:FlyBase.
DR GO; GO:0045727; P:positive regulation of translation; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR045180; La_dom_prot.
DR InterPro; IPR006630; La_HTH.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR22792; PTHR22792; 2.
DR Pfam; PF05383; La; 1.
DR SMART; SM00715; LA; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50961; HTH_LA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..1531
FT /note="La-related protein Larp4B"
FT /id="PRO_0000372865"
FT DOMAIN 262..351
FT /note="HTH La-type RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT DOMAIN 348..423
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 112..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 533..605
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 710..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 748..768
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 791..1135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1160..1211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1251..1285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1393..1418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1450..1531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 801..831
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 832..847
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 861..886
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 916..933
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 948..964
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 977..1050
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1071..1135
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1185..1199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1251..1269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1393..1416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1465..1479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1493..1531
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1123
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1370
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656,
FT ECO:0000269|PubMed:18327897"
FT MOD_RES 1413
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..10
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_037217"
FT CONFLICT 120
FT /note="Q -> QQQH (in Ref. 3; AAQ22587)"
FT /evidence="ECO:0000305"
FT CONFLICT 1439
FT /note="L -> I (in Ref. 3; AAQ22587)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1531 AA; 161994 MW; 77C510044DDC81A9 CRC64;
MEYFLGGYVY MNGDALKIQP PVYTNVPVET AMLATNLFGA TTQIAASPAA AAAAHIPLIT
AAAAAAAAAG APAVAPPVAV APVATASAAQ AAVVPAQQQQ QAHPHQAQIL AHTHVAHQQQ
QQQQQQTIQQ HLHQQQQQQS PHPAQHLTYG HQPALSQATA GGSGTISGGA VGPSGVVVEG
QDTNEYAIMN GALGQTQDGT VVCYTTDALN NTNLVALDPQ PHQIVVPVQV PVQVPVPVQL
PANGSADPQQ GSHNAAGGEE PNIPLDKLKQ MLATQLEYYF SRENLANDTY LLSQMDSDQY
VPIYTVARFN LVRKLTNDIN LITEVLRESP NVQVDDKGLR VRPNRKRCII ILREISNNTP
LDDVKALFSN ESCPRPISCE FAANNSWYIT FESDEDAQKA YKYLREEVKE FQGKPIMARI
KPKSFINRIQ AVPKNGYRLT SPPTANVFDP AGAGGATVSY AAAQQPRYLY TNGAAIAAPA
SVQYSNPVLI PIPQNQFYPG LVAGPWPPGT VAATTAHGQN FYELGGNIFT TNPLPPQPVT
AGFAQAPPPT SQAMVTPKPQ GGGRYNNNHR GNPNNVGGAN SGPRAESRSK PPRSTPSASH
IQGGNIMPIQ ITAPIPGGMV SVVPTNIVQD PLQPAPLQQQ PQQVIQQIQQ QPSSSAQQQQ
AATVQMNNTT RHYPIKSNWK GGMQKNLDKS YSGGVATHHH YTTQVQALPA HSHHLQAQQQ
LQGQTQQQHY QLASSSAASA PQVTYVTTAP APQQPGQHQH HLVVQQTQPQ QQQQQQQVVL
QQQQVQVQEL QEAGDGVEHS SHAMQQVNRS SNMSASSSSS LATSMSKEPL QWQNRPRRRR
RDEEGGINYS PGGRVGLYGS SPSNPHPQQH LMSSSTGSNV QSAGGTDGGG ASHRGGERQS
HYNTIHDVPP PQHRGNYKGN NYNPHYHAQH SSMASGSHHH HHHNALSSQQ QQHHLTTGTG
QQGSGHHYHH HHHHNSIGSN VGNSGGLGVS SGGSGGGGSG GGSGSNSLSG GSNERGIHHS
SLGYQGHQHQ HQQQQQQQQQ QQQQQPAAPV QPPQFDLEDA AFPPLPATSA TAPHTPQATG
GASLHNSTTS SSSSTGLGQK QTLHQQQQQA PQQHQVLSSS VESAGGDEQR SSNQQGIETS
NIHLANSSTA NNWVENRLSD VVRTGGGGGG GGKGKARKDN RHPQGQNQPH MAPNYQQHQP
RNPPVSPTPA LGAEYGIQIQ EGNNTKNVTK QSSSNNNSIH VIKCQTPNIN ASSKEEAAGA
QQQQQQQLDK SNKTEDEMHP KQPSQQRLVE GYVQQQQLPL LAAHNEYSAA LAAGAGACSV
VEGGLTTSLA ECSLGQHKKP PSVAALHAKK DANLLEKGAS KHKSVATSTS TENLSAAATA
SKLSYAQVAQ HHKAAASSDS KETGSGGSTG TLSPTGSHKM DLVFGDPAVV VAAVEKSGLA
TVSTEKRVAG GASPAALIGK PAGGLPATNT TQGSSAVVVS AKEKDNRPNA SVRPLSKEKQ
QQHYGSATEH NTNANIGLGA SGNRKSRANN S
