LARC_ACET2
ID LARC_ACET2 Reviewed; 407 AA.
AC A3DCC8;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Cthe_0369;
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; CP000568; ABN51607.1; -; Genomic_DNA.
DR RefSeq; WP_003512650.1; NC_009012.1.
DR AlphaFoldDB; A3DCC8; -.
DR SMR; A3DCC8; -.
DR STRING; 203119.Cthe_0369; -.
DR PRIDE; A3DCC8; -.
DR EnsemblBacteria; ABN51607; ABN51607; Cthe_0369.
DR KEGG; cth:Cthe_0369; -.
DR eggNOG; COG1641; Bacteria.
DR HOGENOM; CLU_028523_2_1_9; -.
DR OMA; TCLGLDW; -.
DR OrthoDB; 1547060at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel; Reference proteome.
FT CHAIN 1..407
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_1000064645"
SQ SEQUENCE 407 AA; 45633 MW; AC704B990EEE49D2 CRC64;
MRILYFDCFA GASGDMILGA LLDLGIDVGI FKRELAGLNL DGFDIAVEKK VINSIAVTDV
NVIVKEECNH HTGHHHHCER NLADIEKIID ESSLKDNVKR LSKKIFSEIA RAEAKVHNKS
IEDVHFHEVG AIDSIVDIVG TAICLDLLKV DKIYSSPMHD GTGFIECQHG KLPVPVPAVL
EMLKESNIPY ITEDVNTELL TPTGLGIIKC VASKFGPMPP MTIEKVGYGA GKRQTGRFNA
LRCILGNAKE KEKIDDEICM LETNIDDMNP EILGYVMNRL FENGALDVFY TPVYMKKNRP
GVLLTVLTDK EHEEKLVDII LTETTTLGIR KTTAQRYVLE REIKHVNTEF GKIRVKESSF
GDYKKYSPEF EDCKKVAQEL KIPLSKVYDA VNKAILVFEE RNENALQ
