LARC_AKKM8
ID LARC_AKKM8 Reviewed; 406 AA.
AC B2ULM9;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Amuc_1596;
OS Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC
OS 81048 / CCUG 64013 / CIP 107961 / Muc).
OC Bacteria; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales;
OC Akkermansiaceae; Akkermansia.
OX NCBI_TaxID=349741;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP
RC 107961 / Muc;
RX PubMed=21390229; DOI=10.1371/journal.pone.0016876;
RA van Passel M.W., Kant R., Zoetendal E.G., Plugge C.M., Derrien M.,
RA Malfatti S.A., Chain P.S., Woyke T., Palva A., de Vos W.M., Smidt H.;
RT "The genome of Akkermansia muciniphila, a dedicated intestinal mucin
RT degrader, and its use in exploring intestinal metagenomes.";
RL PLoS ONE 6:E16876-E16876(2011).
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; CP001071; ACD05416.1; -; Genomic_DNA.
DR AlphaFoldDB; B2ULM9; -.
DR SMR; B2ULM9; -.
DR STRING; 349741.Amuc_1596; -.
DR EnsemblBacteria; ACD05416; ACD05416; Amuc_1596.
DR KEGG; amu:Amuc_1596; -.
DR eggNOG; COG1641; Bacteria.
DR HOGENOM; CLU_028523_2_1_0; -.
DR OMA; TCLGLDW; -.
DR Proteomes; UP000001031; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel; Reference proteome.
FT CHAIN 1..406
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_1000136685"
SQ SEQUENCE 406 AA; 44217 MW; D01B83ACA702813D CRC64;
MRALVYDCSA GISGDMNLAA LIDLGADREN LERELSKLHV HGEWKLECRP AQQHGIRGTR
IDVLTEEEGH SGSSHAHHHR TMADIRKLIE TSALSNTVKR TALSIFTLLA EAEARVHGTT
SEEVHFHEVG AVDSIIDIAG AAICLEMLRV DTVFTGPVEL GSGTVTCQHG TMPVPAPATA
LLAKHFRATL NGTTHEATTP TGAAYIAALA QPVPSPLAGR ITATGYGIGH RQGLPVPNIL
RVMLVETEEE ETSPELLTEL CANIDDMTPE QTAYLAEKLM EAGALDTWQE SVCMKKGRLA
VKVCALCQPE QTDRVREAFF RHSSTPGIRQ HGMLRHILRR ESAPVHTPHG TVHVKTSFMH
GRPHYRKAEF EDCRILAEKT GLPLGQCQLM GLFPTSSHDN DATDSV
