ID   LARC_ALKMQ              Reviewed;         421 AA.
AC   A6TKJ0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Amet_0481;
OS   Alkaliphilus metalliredigens (strain QYMF).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Alkaliphilus.
OX   NCBI_TaxID=293826;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=QYMF;
RX   PubMed=27811105; DOI=10.1128/genomea.01226-16;
RA   Hwang C., Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina Del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F.,
RA   Land M.L., Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J.,
RA   Richardson P., Fields M.W.;
RT   "Complete genome sequence of Alkaliphilus metalliredigens strain QYMF, an
RT   alkaliphilic and metal-reducing bacterium isolated from borax-contaminated
RT   leachate ponds.";
RL   Genome Announc. 4:0-0(2016).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; CP000724; ABR46708.1; -; Genomic_DNA.
DR   RefSeq; WP_011971616.1; NC_009633.1.
DR   AlphaFoldDB; A6TKJ0; -.
DR   SMR; A6TKJ0; -.
DR   STRING; 293826.Amet_0481; -.
DR   EnsemblBacteria; ABR46708; ABR46708; Amet_0481.
DR   KEGG; amt:Amet_0481; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; TCLGLDW; -.
DR   OrthoDB; 1547060at2; -.
DR   Proteomes; UP000001572; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..421
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000064643"
SQ   SEQUENCE   421 AA;  46928 MW;  7CDC1EFA6EC2ACC7 CRC64;
     MSNQLYLECY SGISGDMAVS ALLDLGADVD VLKKSLHSLN LEGYEINIGR RQKCGIDACY
     FDVVLEGEAN HHHDHQEKHD HHHGGHPVHR NIKDIYEIID NSQISDRSKI LSKKIFHVVA
     KAEAKAHGIA IDEVHFHEVG AVDSIVDIVA AAVCLDNLEI NEVMISDLYE GSGHVKCQHG
     VLPVPVPAVA NIVMDNGLNL RITDTRGELV TPTGAAIAAA IKTKDTLPAS YGIKKIGIGS
     GTKDLPKANI LRAYIIEDNR NYPKKTVAVE DHIDSLDDGE AWVLETNIDD ATGESLGFTM
     ERLLENGAND VFFSPIYMKK NRPAYKLSVI CTKENVKIME SIIFKNTTTI GIRKHKIRRT
     VLKREVTTID TKYGQVRVKV CEFENETYYY PEYEDIKRIC NETGLGFQRV YDEVEKTKTV
     G