ID   LARC_CLOAB              Reviewed;         420 AA.
AC   Q97KZ2;
DT   04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=CA_C0774;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; AE001437; AAK78750.1; -; Genomic_DNA.
DR   PIR; C96995; C96995.
DR   RefSeq; NP_347410.1; NC_003030.1.
DR   RefSeq; WP_010964092.1; NC_003030.1.
DR   AlphaFoldDB; Q97KZ2; -.
DR   SMR; Q97KZ2; -.
DR   STRING; 272562.CA_C0774; -.
DR   PRIDE; Q97KZ2; -.
DR   EnsemblBacteria; AAK78750; AAK78750; CA_C0774.
DR   GeneID; 44997285; -.
DR   KEGG; cac:CA_C0774; -.
DR   PATRIC; fig|272562.8.peg.979; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; TCLGLDW; -.
DR   OrthoDB; 1547060at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..420
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_0000146843"
FT   REGION          81..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   420 AA;  47918 MW;  AB09D4DEC20EB3CD CRC64;
     MRILYYDCFC GISGDMNLAA LVDLGVPKKY LIDELLKLNL GSEYEIKIEK GQKLGITGTR
     VDVILKDEHH NKHELEEVLH NHEHKHNHHE IKNDEPAHSH EHHHRSLRDI EEIINSSTLN
     DKVKKLSLNI FMKVAEAEAK VHGRELYEVH FHEVGAVDSI VDIVGAAICI DYLKVDKILA
     SRVQVGGGFV RCAHGIMPVP APATVEILKN IPINTGIVQF ETTTPTGAAI LAANVKEFTQ
     KLDFIIKKTA YGIGHRDLEI PNVLRVYLGE EESFQDIENQ YILETNIDDM NPEIYGYVEE
     KLFKVGALDV FKTPICMKKG RPGIKLSVLT NEKSEREVLD VIFEETTSIG VRRYKVEKIM
     LKREFSKVKT EFGDITVKKA YYKGELVKYK PEYEECKDIA REKNVSIHKV YKAVYKQDLK