ID   LARC_CLOK5              Reviewed;         399 AA.
AC   A5N505;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=CKL_0332;
OS   Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=431943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX   PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA   Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA   Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA   Hagemeier C., Thauer R.K., Gottschalk G.;
RT   "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT   metabolic features.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000673; EDK32386.1; -; Genomic_DNA.
DR   RefSeq; WP_011988901.1; NC_009706.1.
DR   AlphaFoldDB; A5N505; -.
DR   SMR; A5N505; -.
DR   STRING; 431943.CKL_0332; -.
DR   PRIDE; A5N505; -.
DR   EnsemblBacteria; EDK32386; EDK32386; CKL_0332.
DR   KEGG; ckl:CKL_0332; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; TCLGLDW; -.
DR   OrthoDB; 1547060at2; -.
DR   Proteomes; UP000002411; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..399
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000084509"
SQ   SEQUENCE   399 AA;  45124 MW;  72EE428AE2161537 CRC64;
     MKILYYDCFC GISGDMNLAA LIDLGVPKEY LMEELSKVNL NSEYEMKIER SVKLGITGTR
     VDVKLNESSH GEEEHGHDHY HHHRKLKDIE KIINSSHLSD KVKNISLGMF MKIAEAEAKI
     HGKSLCEVHF HEVGAIDSIV DLVGAAICID YLKVDRIIAS PVQVGGGFVE CSHGIIPVPA
     PATTEILKNI PISTGIVQFE TTTPTGAAIL AVNVEEFTSK IDFSIEKIGY GIGHRDLEIP
     NVLRVYLGEQ ERSEKVEEQY ILETNIDDMN PEFYEYVQER LFEVGALDVF KTPIYMKKGR
     PGINLSVLIS EKGEKDVLDV IFEETTSIGV RKHKVEKIML NRDFSKVKTE YGDITVKKSY
     YKGKLVKYKP EYEECKAIAK EKNISIDKIY KVVYRQDLN