LARC_COREF
ID LARC_COREF Reviewed; 391 AA.
AC Q8FMY8;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=CE2361;
OS Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189
OS / NBRC 100395).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196164;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
RX PubMed=12840036; DOI=10.1101/gr.1285603;
RA Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S.,
RA Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.;
RT "Comparative complete genome sequence analysis of the amino acid
RT replacements responsible for the thermostability of Corynebacterium
RT efficiens.";
RL Genome Res. 13:1572-1579(2003).
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; BA000035; BAC19171.1; -; Genomic_DNA.
DR RefSeq; WP_006768367.1; NZ_GG700683.1.
DR AlphaFoldDB; Q8FMY8; -.
DR SMR; Q8FMY8; -.
DR STRING; 196164.23494204; -.
DR EnsemblBacteria; BAC19171; BAC19171; BAC19171.
DR KEGG; cef:CE2361; -.
DR eggNOG; COG1641; Bacteria.
DR HOGENOM; CLU_028523_2_1_11; -.
DR OMA; TCLGLDW; -.
DR OrthoDB; 1547060at2; -.
DR Proteomes; UP000001409; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel; Reference proteome.
FT CHAIN 1..391
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_0000146844"
SQ SEQUENCE 391 AA; 41558 MW; B7DAA3B5099497A8 CRC64;
MSLWIDASAG VAGDMLLGAL VDAGADLDQI QQVVDAVVPG DVRLTSEVVQ RAGQRCLKVE
VHTTHEHHPH RTWTTIRTML QEADLPDQTH ADALAVFELI AIAEGAVHGV DPETIHFHEV
GALDSIADIV GVCEAIRQLG QTRISSSPIA LGFGRVQAAH GDIPVPVPAV AELARDWPTL
SGAVGGSPEP VGELATPTGV ALVRHFASQA GPLPDGAITT IGVGAGTKDM PGRPNITRVL
LLDDAAPNAD TGKLVQLEAN IDDMDARLWP GVLEQLLDSG ARDAWLTPII MKKGRPAHTI
HVLAAASEVE MVKQVLFAHT TTFGVRSWAV DREGLDRRWE TVHVDGHEVR VKIGSRGGVD
QTTQPEFEDV RAAAQALGIS ESEVLRRAQG Q
