ID   LARC_CORGB              Reviewed;         397 AA.
AC   A4QGM2;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=cgR_2381;
OS   Corynebacterium glutamicum (strain R).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=340322;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R;
RX   PubMed=17379713; DOI=10.1099/mic.0.2006/003657-0;
RA   Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M.,
RA   Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.;
RT   "Comparative analysis of the Corynebacterium glutamicum group and complete
RT   genome sequence of strain R.";
RL   Microbiology 153:1042-1058(2007).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP009044; BAF55388.1; -; Genomic_DNA.
DR   RefSeq; WP_011897772.1; NC_009342.1.
DR   AlphaFoldDB; A4QGM2; -.
DR   SMR; A4QGM2; -.
DR   EnsemblBacteria; BAF55388; BAF55388; cgR_2381.
DR   KEGG; cgt:cgR_2381; -.
DR   HOGENOM; CLU_028523_2_1_11; -.
DR   OMA; TCLGLDW; -.
DR   PhylomeDB; A4QGM2; -.
DR   Proteomes; UP000006698; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel.
FT   CHAIN           1..397
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000064646"
SQ   SEQUENCE   397 AA;  42232 MW;  538CB0EBFA84588E CRC64;
     MGLWIDATAG VAGDMLLGAL IDAGAELEKI QQVVEAVIPG DVLLRAEEVV RQGQRGIKLH
     VEAQHEHHHH RHLSTIKELL VNADIPEQTK QDALGVFELI AIAEGKVHGI DPEKIHFHEV
     GAWDSIADIV GVCEAIRQLN PGLIAASPIA LGFGRIKAAH GDIPVPVPAV AELVKGWPTQ
     TGALMESTEP VGELATPTGV ALIRHFATQD GPFPGGIINE VGIGAGTKDT AGRPNVVRAV
     LFSTSGKAAS NPDTRTLVQL EANVDDQDPR LWTGVIESLL VAGAVDAWLT PILMKKGRPA
     HTVSALVDSS AVEAVKTALF ASTTTFGIRS WEVEREGLDR RFEQVEVDGH TINIKIGSRN
     GHDISAQPEF EDIRSAAVAL GISEREVVAR IPQGTTE