ID   LARC_DESHD              Reviewed;         402 AA.
AC   B8G2K7;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Dhaf_3339;
OS   Desulfitobacterium hafniense (strain DSM 10664 / DCB-2).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=272564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 10664 / DCB-2;
RX   PubMed=22316246; DOI=10.1186/1471-2180-12-21;
RA   Kim S.H., Harzman C., Davis J.K., Hutcheson R., Broderick J.B., Marsh T.L.,
RA   Tiedje J.M.;
RT   "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive
RT   anaerobe capable of dehalogenation and metal reduction.";
RL   BMC Microbiol. 12:21-21(2012).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; CP001336; ACL21357.1; -; Genomic_DNA.
DR   RefSeq; WP_015944549.1; NC_011830.1.
DR   AlphaFoldDB; B8G2K7; -.
DR   SMR; B8G2K7; -.
DR   EnsemblBacteria; ACL21357; ACL21357; Dhaf_3339.
DR   KEGG; dhd:Dhaf_3339; -.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; EILCKHE; -.
DR   Proteomes; UP000007726; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel.
FT   CHAIN           1..402
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000149768"
SQ   SEQUENCE   402 AA;  44761 MW;  98ABCD04EAC2340B CRC64;
     MKAAYLDCFS GISGDMLLGA LVDAGLDFNL LQRDLAGLDL DEYELYEQKV LKQGIRGTQI
     HVHALEGHVH RHLSDIQAII GRSALPPQVK EKSLEIFTRL GKAEAKIHGT DIEQIHFHEV
     GAVDAIVDIV GAVIGFWRLG IEKVFASPIH VGKGFVKAAH GLLPVPAPAT LELLTGVPIY
     AQDVEGELAT PTGAAIVTAY CREFGPFPKI RVERVGYGAG VKDLTIPNLL RLTVGELADE
     DKGQEGIREG EALTLEVNID DMNPECYDYL FEKLFQAGAM DVYIQTIQMK KNRPAVLLTV
     QTPYHKLEEM RKILFQETTT IGLRVYPIKK YMLPYELFTV ETNYGSAKVK VAFMEGRACT
     VSPEYEDCRR LARLTGEPLK QIYEEIKEKA KILLYSTKYP ID