ID   LARC_DESHY              Reviewed;         402 AA.
AC   Q24VE4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=DSY2209;
OS   Desulfitobacterium hafniense (strain Y51).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=138119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y51;
RX   PubMed=16513756; DOI=10.1128/jb.188.6.2262-2274.2006;
RA   Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA   Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT   "Complete genome sequence of the dehalorespiring bacterium
RT   Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT   ethenogenes 195.";
RL   J. Bacteriol. 188:2262-2274(2006).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; AP008230; BAE83998.1; -; Genomic_DNA.
DR   RefSeq; WP_011460167.1; NC_007907.1.
DR   AlphaFoldDB; Q24VE4; -.
DR   SMR; Q24VE4; -.
DR   STRING; 138119.DSY2209; -.
DR   EnsemblBacteria; BAE83998; BAE83998; DSY2209.
DR   KEGG; dsy:DSY2209; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; EILCKHE; -.
DR   OrthoDB; 1547060at2; -.
DR   Proteomes; UP000001946; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..402
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000073016"
SQ   SEQUENCE   402 AA;  44769 MW;  CCF3B6EDF65A7A7D CRC64;
     MKAAYLDCFS GISGDMLLGA LVDAGLDFNL LQRDLAGLDL DEYELYEQKV LKQGIRGTQI
     HVHALEGHVH RHLSDIQAII GRSALPPQVK EKSLEIFTRL GKAEAKIHGT DIEQIHFHEV
     GAVDAIVDIV GAVIGFWRLG IEKVFASPIH VGKGFVKAAH GLLPVPAPAT LELLTGVPIY
     AQDVEGELAT PPGAAIVTAY CREFGPFPKI RVERVGYGAG VKDLTIPNLL RLTVGELADE
     DKGQEGIREG EALTLEVNID DMNPECYDYL FEKLFQAGAM DVYIQTIQMK KNRPAVLLTV
     QTPYHKLEEM RRILFQETTT IGLRVYPIKK YMLPYELLTV ETNYGSAKVK VAFMEGRACT
     VSPEYEDCRR LARLTGEPLK QIYEEIKEKA KILLYSTKYP MD