ID   LARC_GEOKA              Reviewed;         411 AA.
AC   Q5KYI8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=GK1963;
OS   Geobacillus kaustophilus (strain HTA426).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=235909;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT   Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; BA000043; BAD76248.1; -; Genomic_DNA.
DR   RefSeq; WP_011231449.1; NC_006510.1.
DR   AlphaFoldDB; Q5KYI8; -.
DR   SMR; Q5KYI8; -.
DR   STRING; 235909.GK1963; -.
DR   EnsemblBacteria; BAD76248; BAD76248; GK1963.
DR   KEGG; gka:GK1963; -.
DR   PATRIC; fig|235909.7.peg.2103; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_9; -.
DR   OMA; TCLGLDW; -.
DR   Proteomes; UP000001172; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..411
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000064648"
SQ   SEQUENCE   411 AA;  45196 MW;  D24372485CC1D493 CRC64;
     MKILYLDCFS GISGDMMVGA LVDAGVPSEK IEVELKKLSL SGYKLQWGKV VKKGISATKF
     DVIIQESIEG GHHHQSHHAH RHYSDIIDMI RRSELSEEVK QRAGQIFHCI AVAEAKIHGI
     PVENVHFHEV GAVDSIVDIV ATAIALTELH IDYIISAPIP LGHGSIRCAH GVYPVPAPAT
     LEILQGVPVV SNHLPFELTT PTGAGIVKSQ VNSYGPLPSM KISAIGYGAG TRDLPDQPNV
     LRVVIGEETR ITSSGPSLFH SKEETVYILE CQLDDMPGEA LGYVMDGLFQ KGALDVFYTP
     VFMKKNRPGV LLTVLTSATH VEQCEQFMLK ETTTLGIRKD IWVREVLERD VVTVATSYGN
     IRVKQAIHKG KVIRQMPEYE DVKEAALTHQ VAFLDVYAEA AEQARRRIKG E