ID   LARC_NOCSJ              Reviewed;         393 AA.
AC   A1SD28;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE            EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE   AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN   Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Noca_0168;
OS   Nocardioides sp. (strain ATCC BAA-499 / JS614).
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Nocardioides.
OX   NCBI_TaxID=196162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-499 / JS614;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J.,
RA   Richardson P.;
RT   "Complete sequence of chromosome 1 of Nocardioides sp. JS614.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC       ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC       delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC       (P2TMN), to form the mature cofactor. Is thus probably required for the
CC       activation of nickel-pincer cofactor-dependent enzymes.
CC       {ECO:0000255|HAMAP-Rule:MF_01074}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC         Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC         Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC         ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01074};
CC   -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01074}.
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DR   EMBL; CP000509; ABL79713.1; -; Genomic_DNA.
DR   RefSeq; WP_011753664.1; NC_008699.1.
DR   AlphaFoldDB; A1SD28; -.
DR   SMR; A1SD28; -.
DR   STRING; 196162.Noca_0168; -.
DR   EnsemblBacteria; ABL79713; ABL79713; Noca_0168.
DR   KEGG; nca:Noca_0168; -.
DR   eggNOG; COG1641; Bacteria.
DR   HOGENOM; CLU_028523_2_1_11; -.
DR   OMA; TCLGLDW; -.
DR   OrthoDB; 1547060at2; -.
DR   Proteomes; UP000000640; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01074; LarC; 1.
DR   InterPro; IPR002822; Ni_insertion.
DR   PANTHER; PTHR36566; PTHR36566; 1.
DR   Pfam; PF01969; DUF111; 1.
DR   TIGRFAMs; TIGR00299; TIGR00299; 1.
PE   3: Inferred from homology;
KW   Lyase; Nickel; Reference proteome.
FT   CHAIN           1..393
FT                   /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT                   nickel insertion protein"
FT                   /id="PRO_1000064655"
SQ   SEQUENCE   393 AA;  40240 MW;  91B03F5D01691CF4 CRC64;
     MTTAWVDASA GASGDMLLGA LVGAGVPVAV LQAAVDAVAP EPVTLRVEHV RRGGLAATRC
     HVEVTDTARH RTWRDVRTLL AAADLADDVR GLALRVFERL ATAEATVHGT SPDDVHFHEV
     GALDAIADVT GVVAGFVHLG ATAVTVSPVA VGSGSVSTAH GLLPVPPPAV AELLRGVPSY
     AGAATMETCT PTGAALLTTL ATAYGPQPAM SVDTIGVGAG GRDPEGHPNV LRLFVGVPAD
     AGGGPLLLEC NVDDLDPRVW PAVIAALLEA GASDAWLTPI LMKKGRPAHT LSALVDAGRA
     AGVRAAIFRQ TSTIGLREQP LTKHALEREI VAVDVDGQRI AVKLARHDGA VVNAQPEYDD
     VARAAADLDR PVADVLAEAV ARGRAFLTPG DKI