LARC_STAES
ID LARC_STAES Reviewed; 395 AA.
AC Q8CTP4;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=SE_0371;
OS Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=176280;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 12228 / FDA PCI 1200;
RX PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT "Genome-based analysis of virulence genes in a non-biofilm-forming
RT Staphylococcus epidermidis strain (ATCC 12228).";
RL Mol. Microbiol. 49:1577-1593(2003).
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; AE015929; AAO03968.1; -; Genomic_DNA.
DR RefSeq; NP_763926.1; NC_004461.1.
DR RefSeq; WP_002438754.1; NZ_WBME01000026.1.
DR AlphaFoldDB; Q8CTP4; -.
DR SMR; Q8CTP4; -.
DR STRING; 176280.SE_0371; -.
DR EnsemblBacteria; AAO03968; AAO03968; SE_0371.
DR GeneID; 50019467; -.
DR KEGG; sep:SE_0371; -.
DR PATRIC; fig|176280.10.peg.346; -.
DR eggNOG; COG1641; Bacteria.
DR HOGENOM; CLU_028523_2_1_9; -.
DR OMA; TCLGLDW; -.
DR Proteomes; UP000001411; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel.
FT CHAIN 1..395
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_0000146851"
SQ SEQUENCE 395 AA; 44129 MW; B5253CF50712206D CRC64;
MTKALYLDCH AGIAGDMLLS ALVDLGANPE DIESELKKLP LDQFKLHFQK RVKQGIHAMT
LNIDVKEANH HRHVNDIFKM IDDSTLPERV KYRSKKIFEI IGQAEAKIHG MSFEEVHFHE
VGAMDSIIDI IGGCIALEQL GINTLYCSAI PTGHGKINIA HGIYPIPAPA TAEILKGIPI
AHFDVQSELT TPTGAAFAKG LVSSFGPFPS ATIQHIGYGA GSKDFDFPNI LRVIQFESEF
EQQDSVQVIE CQIDDMTPEA LGYFMNNALE QGALDAYYTP IFMKKSRPST QLTLICKLHD
KTYFEQLILQ ETSSLGVRST SVNRKTLNRA FKILSTQHGT VSIKFGLQNG KIMKMKPEYE
DLKKIAKTTK QPFQVIHNEV LQQLYQTYHI GNILQ
