LARC_THEMA
ID LARC_THEMA Reviewed; 402 AA.
AC Q9X059;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=TM_0964;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000512; AAD36043.1; -; Genomic_DNA.
DR PIR; A72312; A72312.
DR RefSeq; NP_228772.1; NC_000853.1.
DR RefSeq; WP_004080598.1; NZ_CP011107.1.
DR AlphaFoldDB; Q9X059; -.
DR SMR; Q9X059; -.
DR STRING; 243274.THEMA_09525; -.
DR EnsemblBacteria; AAD36043; AAD36043; TM_0964.
DR KEGG; tma:TM0964; -.
DR eggNOG; COG1641; Bacteria.
DR InParanoid; Q9X059; -.
DR OMA; TCLGLDW; -.
DR OrthoDB; 1547060at2; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel; Reference proteome.
FT CHAIN 1..402
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_0000146856"
SQ SEQUENCE 402 AA; 45064 MW; B712DE541A0E30A3 CRC64;
MRILYLDPFS GISGDMFLGL LVDLGVDPEK IKSRLEKLNV EFDLVVKKVN KKGVTATKVD
VVFPRKEHHE DHIVSDHDHH HHHGRHLSEI VEVLSRLEDP LKEKAIRMFE ALAEAESKIH
GLSKEKVHFH EVGAMDAVIE IAGAVVGLEL LEVEKVFCGT VNTGSGFVMT EHGRYPVPAP
ATAELLKGIP IYMDQKVRAE LVTPTGAVIL KILVDEFRTP ILRVEKVGYG AGTMDLEIPN
VLRGYLGYIE PSERTGDVLI ETNVDDMNPQ LFGHLMERLF EAGAKDVFFT PIYMKKNRPA
VKVSVLCHES KKDEILKLLF KESTSIGARV FYPEKVEATR TLKTVKTEYG EIPVKIASFD
SEIVNISPEY EACKKVAQEK GIPLKEVYRA VYKSVSEVRN DV
