LARC_THEP1
ID LARC_THEP1 Reviewed; 397 AA.
AC A5INL0;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000255|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000255|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000255|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000255|HAMAP-Rule:MF_01074}; OrderedLocusNames=Tpet_1783;
OS Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 /
OS RKU-1).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=390874;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C.,
RA Tapia R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT "Complete sequence of Thermotoga petrophila RKU-1.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000255|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000255|HAMAP-
CC Rule:MF_01074}.
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DR EMBL; CP000702; ABQ47783.1; -; Genomic_DNA.
DR RefSeq; WP_011944187.1; NC_009486.1.
DR AlphaFoldDB; A5INL0; -.
DR SMR; A5INL0; -.
DR STRING; 390874.Tpet_1783; -.
DR EnsemblBacteria; ABQ47783; ABQ47783; Tpet_1783.
DR KEGG; tpt:Tpet_1783; -.
DR eggNOG; COG1641; Bacteria.
DR HOGENOM; CLU_028523_2_1_0; -.
DR OMA; TCLGLDW; -.
DR Proteomes; UP000006558; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR PANTHER; PTHR36566; PTHR36566; 1.
DR Pfam; PF01969; DUF111; 1.
DR TIGRFAMs; TIGR00299; TIGR00299; 1.
PE 3: Inferred from homology;
KW Lyase; Nickel.
FT CHAIN 1..397
FT /note="Pyridinium-3,5-bisthiocarboxylic acid mononucleotide
FT nickel insertion protein"
FT /id="PRO_1000136696"
SQ SEQUENCE 397 AA; 44444 MW; 2322F9899270A0F7 CRC64;
MEKILYLDPF SGIAGDMFLG LLVDLGVDPE ELKKRLSKLG VEFELRIRKV NKKGITATKV
DVVFPGKEHH EDHIDHEHHG RHLSEIMKIL ERLEDPAREK ATEMFETLAE AESKVHGLPK
EKVHFHEVGA MDAVIEIAGA VVGLELLGVE RVFCGAVNTG SGFVMTEHGR YPVPAPATAE
LLKGIPIYMD QKVRAELVTP TGAVILKELV DEFGTPILRI EKVGYGAGTV DLEIPNVLRG
YLGFLEGKSE RDILIETNVD DMNPQLFGYL MEKLFETGAK DVFYTPIYMK KNRPAVKVSV
LCSEEKRDQI LKVLFKESTS IGARVFHLEK VEAPRKVISV ETEYGKIPVK VAYFDSEVVN
VSPEYEACKK IAQEKEVPLK EIYNAVYRKI SEVQGNV
