LARGN_MOUSE
ID LARGN_MOUSE Reviewed; 304 AA.
AC A3KMN5; Q8BLB9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Protein Largen;
DE AltName: Full=Proline-rich protein 16;
GN Name=Prr16;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION.
RX PubMed=24656129; DOI=10.1016/j.molcel.2014.02.028;
RA Yamamoto K., Gandin V., Sasaki M., McCracken S., Li W., Silvester J.L.,
RA Elia A.J., Wang F., Wakutani Y., Alexandrova R., Oo Y.D., Mullen P.J.,
RA Inoue S., Itsumi M., Lapin V., Haight J., Wakeham A., Shahinian A.,
RA Ikura M., Topisirovic I., Sonenberg N., Mak T.W.;
RT "Largen: a molecular regulator of mammalian cell size control.";
RL Mol. Cell 53:904-915(2014).
CC -!- FUNCTION: Regulator of cell size that promotes cell size increase
CC independently of mTOR and Hippo signaling pathways. Acts by stimulating
CC the translation of specific mRNAs, including those encoding proteins
CC affecting mitochondrial functions. Increases mitochondrial mass and
CC respiration (Probable). {ECO:0000305|PubMed:24656129}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A3KMN5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A3KMN5-2; Sequence=VSP_028882;
CC -!- MISCELLANEOUS: Overexpression causes embryonic lethality. Conditional
CC overexpression leads to increased cell size (PubMed:24656129).
CC {ECO:0000305|PubMed:24656129}.
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DR EMBL; AK045646; BAC32443.1; -; mRNA.
DR EMBL; BC132481; AAI32482.1; -; mRNA.
DR CCDS; CCDS37818.1; -. [A3KMN5-1]
DR RefSeq; NP_001074693.1; NM_001081224.2. [A3KMN5-1]
DR AlphaFoldDB; A3KMN5; -.
DR SMR; A3KMN5; -.
DR BioGRID; 214671; 1.
DR STRING; 10090.ENSMUSP00000112338; -.
DR PhosphoSitePlus; A3KMN5; -.
DR MaxQB; A3KMN5; -.
DR PaxDb; A3KMN5; -.
DR PRIDE; A3KMN5; -.
DR ProteomicsDB; 264973; -. [A3KMN5-1]
DR ProteomicsDB; 264974; -. [A3KMN5-2]
DR Antibodypedia; 25588; 106 antibodies from 18 providers.
DR Ensembl; ENSMUST00000116639; ENSMUSP00000112338; ENSMUSG00000073565. [A3KMN5-1]
DR Ensembl; ENSMUST00000235541; ENSMUSP00000158421; ENSMUSG00000073565. [A3KMN5-1]
DR GeneID; 71373; -.
DR KEGG; mmu:71373; -.
DR UCSC; uc008ewz.1; mouse. [A3KMN5-1]
DR CTD; 51334; -.
DR MGI; MGI:1918623; Prr16.
DR VEuPathDB; HostDB:ENSMUSG00000073565; -.
DR eggNOG; ENOG502QQGT; Eukaryota.
DR GeneTree; ENSGT00910000144204; -.
DR HOGENOM; CLU_073752_0_0_1; -.
DR InParanoid; A3KMN5; -.
DR OMA; DCDVRYN; -.
DR OrthoDB; 1282387at2759; -.
DR PhylomeDB; A3KMN5; -.
DR TreeFam; TF332746; -.
DR BioGRID-ORCS; 71373; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Prr16; mouse.
DR PRO; PR:A3KMN5; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; A3KMN5; protein.
DR Bgee; ENSMUSG00000073565; Expressed in epididymal fat pad and 76 other tissues.
DR Genevisible; A3KMN5; MM.
DR GO; GO:0045793; P:positive regulation of cell size; IDA:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
DR InterPro; IPR027997; Largen/INSYN1.
DR PANTHER; PTHR15917; PTHR15917; 1.
DR Pfam; PF15252; DUF4589; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Reference proteome.
FT CHAIN 1..304
FT /note="Protein Largen"
FT /id="PRO_0000308160"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 33..70
FT /evidence="ECO:0000255"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..132
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..260
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..291
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..70
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028882"
SQ SEQUENCE 304 AA; 32836 MW; E0BDD35521784A55 CRC64;
MSAKSKGNPS SSSAAEGPPA ASKTKVKEQI KIIVEDLELV LGDLKDVAKE LKEVVDQIDT
LTSDLQLEDE MTDSSKTDTL NSSSSGTTAS SIEKIKEQAN APLIKPPAHP SAILTVLRKP
NPPPPPPRLT PVRCEEPQRV VPTANPVKTN GTLLRNGGLA GRPNKIPNGD ICCIPNSNLD
KAPLQSLMHR PEKDRSPQAG PRERVRFNEK VQYHGYCPDC ESRYNIKNRE VHLHSEPVHP
PGKIPHQGPL LHPPPHLPNF PLENGGLGIS HSNSFPPPTP ATVPPPAAPK PQKTILRKST
TTTV
