LARO_LACPL
ID LARO_LACPL Reviewed; 241 AA.
AC Q890D1; F9USS5;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Nickel import ATP-binding protein LarO {ECO:0000305};
DE EC=7.2.2.- {ECO:0000305};
DE AltName: Full=Energy-coupling factor transporter ATP-binding protein LarO {ECO:0000305};
DE Short=ECF transporter A component LarO {ECO:0000305};
DE AltName: Full=Nickel transport ATP-binding protein {ECO:0000303|PubMed:24710389};
GN Name=larO {ECO:0000303|PubMed:24710389}; OrderedLocusNames=lp_0100;
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=24710389; DOI=10.1038/ncomms4615;
RA Desguin B., Goffin P., Viaene E., Kleerebezem M., Martin-Diaconescu V.,
RA Maroney M.J., Declercq J.P., Soumillion P., Hols P.;
RT "Lactate racemase is a nickel-dependent enzyme activated by a widespread
RT maturation system.";
RL Nat. Commun. 5:3615-3615(2014).
CC -!- FUNCTION: Probable ATP-binding component of the energy-coupling factor
CC (ECF) transporter complex LarMNQO involved in nickel import. LarO is
CC presumably responsible for energy coupling to the transport system.
CC {ECO:0000305|PubMed:24710389}.
CC -!- SUBUNIT: May form an energy-coupling factor (ECF) transporter complex
CC composed of an ATP-binding protein (A component, LarO), a transmembrane
CC protein (T component, LarQ) and a fused possible substrate-capture
CC protein (S component, LarMN) of unknown stoichiometry. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A2RI02};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:A2RI02}.
CC -!- INDUCTION: Induced by L-lactate but not by a racemic mixture of DL-
CC lactate. Makes part of the larR(MN)QO operon.
CC {ECO:0000269|PubMed:24710389}.
CC -!- DISRUPTION PHENOTYPE: Deletion of both larO and larQ leads to a loss of
CC lactate racemase activity. Addition of Ni(2+) (but not Co(2+)) restores
CC lactate racemase activity in this mutant.
CC {ECO:0000269|PubMed:24710389}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; AL935263; CCC77656.1; -; Genomic_DNA.
DR RefSeq; YP_004888170.1; NC_004567.2.
DR AlphaFoldDB; Q890D1; -.
DR SMR; Q890D1; -.
DR STRING; 220668.lp_0100; -.
DR EnsemblBacteria; CCC77656; CCC77656; lp_0100.
DR KEGG; lpl:lp_0100; -.
DR PATRIC; fig|220668.9.peg.80; -.
DR eggNOG; COG1122; Bacteria.
DR HOGENOM; CLU_000604_1_22_9; -.
DR PhylomeDB; Q890D1; -.
DR BioCyc; LPLA220668:G1GW0-78-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell membrane; Ion transport; Membrane; Nickel;
KW Nickel transport; Nucleotide-binding; Reference proteome; Translocase;
KW Transport.
FT CHAIN 1..241
FT /note="Nickel import ATP-binding protein LarO"
FT /id="PRO_0000092023"
FT DOMAIN 2..240
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 241 AA; 26336 MW; 84E826265AC7A5BC CRC64;
MIKLVNICYD YPDTCGLKDL SLTVNSGDFI CLMGPNGSGK STLLRLLSGL ASPTSGAYQF
HDQPITTTYL ADAQNRQQLH QRIGMVFQNT DVQLFNTSVT EEVAFGPRQL GLSAAMVAQR
VADCLQLTDC ANLADRVPYQ LSGGEKKRVA LASVLALNPE ILLLDEPLNG LTIAAQQQML
TLLQRLQAAG KTIIMASHNY QQVQAVGERF IIFNSTHQVD ADLTRADLDQ QPARQAQLMT
L
