ID   LARP1_CRYNH             Reviewed;         895 AA.
AC   J9VT60;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=La RNA-binding domain-containing protein LHP1 {ECO:0000305};
GN   Name=LHP1 {ECO:0000303|PubMed:27611567};
GN   ORFNames=CNAG_04570 {ECO:0000312|EMBL:AFR97647.1};
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN   [1] {ECO:0000312|Proteomes:UP000010091}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2] {ECO:0000305}
RP   IDENTIFICATION BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE, AND
RP   PHOSPHORYLATION AT SER-847.
RX   PubMed=27611567; DOI=10.1371/journal.ppat.1005873;
RA   Park H.S., Chow E.W., Fu C., Soderblom E.J., Moseley M.A., Heitman J.,
RA   Cardenas M.E.;
RT   "Calcineurin Targets Involved in Stress Survival and Fungal Virulence.";
RL   PLoS Pathog. 12:e1005873-e1005873(2016).
CC   -!- FUNCTION: May act as an RNA-binding protein. {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Sensitive to various stresses including heat,
CC       sodium dodecyl sulfate and calcineurin inhibitor tacrolimus
CC       (PubMed:27611567). Resistant to tunicamycin-induced ER stress
CC       (PubMed:27611567). Simultaneous disruption of CRZ1 enhances
CC       thermosensitivity and decreases virulence in a mouse intranasal
CC       inhalation model for pulmonary infection (PubMed:27611567).
CC       {ECO:0000269|PubMed:27611567}.
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DR   EMBL; CP003829; AFR97647.1; -; Genomic_DNA.
DR   RefSeq; XP_012052065.1; XM_012196675.1.
DR   AlphaFoldDB; J9VT60; -.
DR   SMR; J9VT60; -.
DR   SwissPalm; J9VT60; -.
DR   EnsemblFungi; AFR97647; AFR97647; CNAG_04570.
DR   GeneID; 23887966; -.
DR   VEuPathDB; FungiDB:CNAG_04570; -.
DR   HOGENOM; CLU_324910_0_0_1; -.
DR   Proteomes; UP000010091; Chromosome 10.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR045180; La_dom_prot.
DR   InterPro; IPR006630; La_HTH.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR22792; PTHR22792; 1.
DR   Pfam; PF05383; La; 1.
DR   SMART; SM00715; LA; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50961; HTH_LA; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; RNA-binding.
FT   CHAIN           1..895
FT                   /note="La RNA-binding domain-containing protein LHP1"
FT                   /id="PRO_0000451160"
FT   DOMAIN          706..796
FT                   /note="HTH La-type RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT   REGION          24..265
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          285..344
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          359..590
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          796..857
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          870..895
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        27..75
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..144
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        147..220
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        221..243
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        359..374
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        404..426
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        447..465
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        497..556
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        872..888
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         847
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:27611567"
SQ   SEQUENCE   895 AA;  93777 MW;  044321E6B0709B31 CRC64;
     MSPSQQQTSV FNALGSYADR IKDANGNFIK SSPSSSSSAT PEPTSLSSST SGKKAFSTAT
     SKSGQQKQGS SPQPGAITAE DDGPWETVQS TRARQRTDRS EEKEKRGSSS KNWRERSHRD
     EKNQDDGEKR NGRERSKKEK GDKSNSAPFV GSATSSEKTA KSLSSSTKNA WGVTSSSQGE
     NPIASNPGPK QKAQNDSTFR SSSAAAPVGP TTSTINEIIK QSEGSDEDNW RARPAKVEKN
     EKTEGPVSIT QAQPQPQRQL APPPSVNIWD LRKKMSVPVL VSPTSATSAV AGIPPKSDKE
     KSLTNGMVKE EDSGTGKSLS KKKSAAAAAA AGTSSTPPSI HDATLWPDII QAAEVVKAGE
     DKKEKAKERL NSESASVAEE STIGIGKKPK WTPIPAHELL AAADHAAEQS RRQNRMEAKK
     RSSGREGGES GPTGSGAPGK SNKTRKGMPA AEGKKARKEG AQQKDGHASS KAGETIGAGT
     GKANGDVKET KEGDARSASQ QESSSHRSGP SISASANTGI DSSLHARTKS TPNASTTPLP
     PHTFNLASSS NLPRSTRGRG EGRGSFGGGR ARGGFRSSGA LGHKGQLGHG YGHVHGQGQG
     FGYGYGSPPM GVAGLPVEGI IYAPLNPGAG AGAGASPNLY QRGYGMGFQP FYPAATAAAS
     AGEGGPTGAA AGDAAGVYDP AAAVYGNMGM YKSASMPPPP MPQTVVPNLD PLRFYVLGQV
     EYYFSMQNLA MDFFLRQQMD SEGWIDIAMI ASFNRIKSLT PETSIVRECM ILSNYLEVRE
     DKVRLSGAES HRWVLPDAAP SKFGPDPRSP SLAEGAESSE ERDGAASGSQ SGLVTAGEEG
     AQGLQASPRR MFGAQDVKDA LMKSSALSTV NGEIKEKEEV KAMENEGEES ENYEQ