LARQ_LACPL
ID LARQ_LACPL Reviewed; 273 AA.
AC F9USS6;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Nickel permease LarQ {ECO:0000303|PubMed:24710389};
DE AltName: Full=Energy-coupling factor transporter transmembrane protein LarQ {ECO:0000305};
DE Short=ECF transporter T component LarQ {ECO:0000305};
GN Name=larQ {ECO:0000303|PubMed:24710389};
GN OrderedLocusNames=lp_0101 {ECO:0000312|EMBL:CCC77657.1};
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=24710389; DOI=10.1038/ncomms4615;
RA Desguin B., Goffin P., Viaene E., Kleerebezem M., Martin-Diaconescu V.,
RA Maroney M.J., Declercq J.P., Soumillion P., Hols P.;
RT "Lactate racemase is a nickel-dependent enzyme activated by a widespread
RT maturation system.";
RL Nat. Commun. 5:3615-3615(2014).
CC -!- FUNCTION: Probable transmembrane component of the energy-coupling
CC factor (ECF) transporter complex LarMNQO involved in nickel import.
CC {ECO:0000305|PubMed:24710389}.
CC -!- SUBUNIT: May form an energy-coupling factor (ECF) transporter complex
CC composed of an ATP-binding protein (A component, LarO), a transmembrane
CC protein (T component, LarQ) and a fused possible substrate-capture
CC protein (S component, LarMN) of unknown stoichiometry. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced by L-lactate but not by a racemic mixture of DL-
CC lactate. Makes part of the larR(MN)QO operon.
CC {ECO:0000269|PubMed:24710389}.
CC -!- DISRUPTION PHENOTYPE: Deletion of both larO and larQ leads to a loss of
CC lactate racemase activity. Addition of Ni(2+) (but not Co(2+)) restores
CC lactate racemase activity in this mutant.
CC {ECO:0000269|PubMed:24710389}.
CC -!- SIMILARITY: Belongs to the CbiQ family. {ECO:0000305}.
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DR EMBL; AL935263; CCC77657.1; -; Genomic_DNA.
DR RefSeq; WP_011100882.1; NC_004567.2.
DR RefSeq; YP_004888171.1; NC_004567.2.
DR AlphaFoldDB; F9USS6; -.
DR SMR; F9USS6; -.
DR STRING; 220668.lp_0101; -.
DR EnsemblBacteria; CCC77657; CCC77657; lp_0101.
DR GeneID; 57026943; -.
DR KEGG; lpl:lp_0101; -.
DR PATRIC; fig|220668.9.peg.81; -.
DR eggNOG; COG0619; Bacteria.
DR HOGENOM; CLU_056469_3_0_9; -.
DR OMA; MECRGFT; -.
DR BioCyc; LPLA220668:G1GW0-79-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR InterPro; IPR003339; ABC/ECF_trnsptr_transmembrane.
DR Pfam; PF02361; CbiQ; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..273
FT /note="Nickel permease LarQ"
FT /id="PRO_0000441650"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 273 AA; 31307 MW; 8C9C4D998A9AD479 CRC64;
MKPTRPNTDI PAWLQTTTTT PTPAIKAKFW QRNQRHLRQL LSRLAQPAPV TATSHWRVAP
QFKLIQLLLL VILIALSNNL ILLWSLALLV GCQLLWLPPR QLRRFMGSWL ISVGMAMLFV
LPSYWLAGPT TLLFFGLKTS LMLANAQYYR LTTPFQDLLA GLKALHCPDL LIMTLAIAIT
YLRMLGQHLL LTMEALELRT VAPTAHPYRL IGALFGNLYL KSYTYALELY AAMEARGFNG
HYVRSTGRRT HWRDYLALSP AIIVWILFIF WRH
