ID   LARR_LACPL              Reviewed;         221 AA.
AC   F9USS8;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Lactate racemization regulatory protein {ECO:0000305|PubMed:25349156};
DE   AltName: Full=Transcriptional activator LarR {ECO:0000305|PubMed:25349156};
GN   Name=larR {ECO:0000303|PubMed:24710389};
GN   OrderedLocusNames=lp_0103 {ECO:0000312|EMBL:CCC77659.1};
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
RN   [3]
RP   INDUCTION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=24710389; DOI=10.1038/ncomms4615;
RA   Desguin B., Goffin P., Viaene E., Kleerebezem M., Martin-Diaconescu V.,
RA   Maroney M.J., Declercq J.P., Soumillion P., Hols P.;
RT   "Lactate racemase is a nickel-dependent enzyme activated by a widespread
RT   maturation system.";
RL   Nat. Commun. 5:3615-3615(2014).
RN   [4]
RP   FUNCTION, ACTIVITY REGULATION, SUBUNIT, AND DNA-BINDING.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=25349156; DOI=10.1128/jb.02192-14;
RA   Desguin B., Goffin P., Bakouche N., Diman A., Viaene E., Dandoy D.,
RA   Fontaine L., Hallet B., Hols P.;
RT   "Enantioselective regulation of lactate racemization by LarR in
RT   Lactobacillus plantarum.";
RL   J. Bacteriol. 197:219-230(2015).
CC   -!- FUNCTION: Positive transcriptional regulator that is absolutely
CC       required for the expression of lactate racemase (Lar) activity.
CC       Controls Lar expression by sensing the L-/D-lactate ration. Binds to a
CC       16-bp palindromic sequence (Lar box motif) that is present in the larR-
CC       larA intergenic region, allowing transcription of the larABCDE operon.
CC       {ECO:0000269|PubMed:25349156}.
CC   -!- ACTIVITY REGULATION: L-lactate acts as a positive effector on the
CC       binding and multimerization of LarR on DNA, while D-lactate antagonizes
CC       the positive effect of L-lactate. {ECO:0000269|PubMed:25349156}.
CC   -!- SUBUNIT: Multimerizes on DNA. Multimerization is required for
CC       transcription activation. {ECO:0000269|PubMed:25349156}.
CC   -!- INDUCTION: Induced by L-lactate but not by a racemic mixture of DL-
CC       lactate. Makes part of the larR(MN)QO operon.
CC       {ECO:0000269|PubMed:24710389}.
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DR   EMBL; AL935263; CCC77659.1; -; Genomic_DNA.
DR   RefSeq; WP_003641710.1; NC_004567.2.
DR   RefSeq; YP_004888173.1; NC_004567.2.
DR   AlphaFoldDB; F9USS8; -.
DR   SMR; F9USS8; -.
DR   STRING; 220668.lp_0103; -.
DR   DNASU; 1061368; -.
DR   EnsemblBacteria; CCC77659; CCC77659; lp_0103.
DR   GeneID; 57026945; -.
DR   GeneID; 66448243; -.
DR   KEGG; lpl:lp_0103; -.
DR   PATRIC; fig|220668.9.peg.83; -.
DR   eggNOG; COG0664; Bacteria.
DR   HOGENOM; CLU_075053_12_0_9; -.
DR   OMA; MTMNGKK; -.
DR   PhylomeDB; F9USS8; -.
DR   BioCyc; LPLA220668:G1GW0-81-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF13545; HTH_Crp_2; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..221
FT                   /note="Lactate racemization regulatory protein"
FT                   /id="PRO_0000441648"
FT   DOMAIN          139..213
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        172..192
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
SQ   SEQUENCE   221 AA;  25692 MW;  BB04358320261234 CRC64;
     MVLTDIEYLL SYLEAHHVPT IKKKRHTYLT YHGLAEHYTY VLKDGIIKNS IILQDGREYN
     LSYIAKPDVI SLLRDEVSRS TDQPFNVRIE SEYATFYQVN RVAFWKYVNS TPELQNYVKN
     YYRKKLSENI LRLQRMVMNG KKGAICAFIY SLVDLFGRKV NEGILIDFVV TNDDIAGFCG
     ISSRSSVNRM LKELRTDGVI TVKNHKFIIQ DVSYLLDQIA N