ID   LAR_DESUN               Reviewed;         382 AA.
AC   Q84V83;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Leucoanthocyanidin reductase;
DE            Short=Leucocyanidin reductase;
DE            EC=1.17.1.3;
GN   Name=LAR;
OS   Desmodium uncinatum (Silverleaf Spanish clover) (Hedysarum uncinatum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Desmodieae; Desmodium.
OX   NCBI_TaxID=225101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RC   TISSUE=Leaf;
RX   PubMed=12788945; DOI=10.1074/jbc.m302783200;
RA   Tanner G.J., Francki K.T., Abrahams S., Watson J.M., Larkin P.J.,
RA   Ashton A.R.;
RT   "Proanthocyanidin biosynthesis in plants. Purification of legume
RT   leucoanthocyanidin reductase and molecular cloning of its cDNA.";
RL   J. Biol. Chem. 278:31647-31656(2003).
CC   -!- FUNCTION: Catalyzes the synthesis of catechin from 3,4-cis-
CC       leucocyanidin. Also synthesizes afzelechin and gallocatechin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3S)-catechin + H2O + NADP(+) = (2R,3S,4S)-leucocyanidin +
CC         H(+) + NADPH; Xref=Rhea:RHEA:10616, ChEBI:CHEBI:11412,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15600,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.17.1.3;
CC   -!- PATHWAY: Flavonoid metabolism; proanthocyanidin biosynthesis.
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone
CC       reductase subfamily. {ECO:0000305}.
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DR   EMBL; AJ550154; CAD79341.1; -; mRNA.
DR   AlphaFoldDB; Q84V83; -.
DR   SMR; Q84V83; -.
DR   KEGG; ag:CAD79341; -.
DR   BRENDA; 1.17.1.3; 1878.
DR   UniPathway; UPA00952; -.
DR   GO; GO:0033788; F:leucoanthocyanidin reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009807; P:lignan biosynthetic process; IEA:UniProt.
DR   GO; GO:0010023; P:proanthocyanidin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd05259; PCBER_SDR_a; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR008030; NmrA-like.
DR   InterPro; IPR045312; PCBER-like.
DR   Pfam; PF05368; NmrA; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NADP; Oxidoreductase.
FT   CHAIN           1..382
FT                   /note="Leucoanthocyanidin reductase"
FT                   /id="PRO_0000204553"
FT   ACT_SITE        142
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         19..25
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         44
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         52
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         146
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
SQ   SEQUENCE   382 AA;  42665 MW;  19CCF6B619FED368 CRC64;
     MTVSGAIPSM TKNRTLVVGG TGFIGQFITK ASLGFGYPTF LLVRPGPVSP SKAVIIKTFQ
     DKGAKVIYGV INDKECMEKI LKEYEIDVVI SLVGGARLLD QLTLLEAIKS VKTIKRFLPS
     EFGHDVDRTD PVEPGLTMYK EKRLVRRAVE EYGIPFTNIC CNSIASWPYY DNCHPSQVPP
     PMDQFQIYGD GNTKAYFIDG NDIGKFTMKT IDDIRTLNKN VHFRPSSNCY SINELASLWE
     KKIGRTLPRF TVTADKLLAH AAENIIPESI VSSFTHDIFI NGCQVNFSID EHSDVEIDTL
     YPDEKFRSLD DCYEDFVPMV HDKIHAGKSG EIKIKDGKPL VQTGTIEEIN KDIKTLVETQ
     PNEEIKKDMK ALVEAVPISA MG