LAS1_YEAST
ID LAS1_YEAST Reviewed; 502 AA.
AC P36146; D6VXC4; Q66R55;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein LAS1;
GN Name=LAS1; OrderedLocusNames=YKR063C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=S288c / GRFAT8;
RX PubMed=8582632; DOI=10.1093/genetics/141.3.857;
RA Doseff A.I., Arndt K.T.;
RT "LAS1 is an essential nuclear protein involved in cell morphogenesis and
RT cell surface growth.";
RL Genetics 141:857-871(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Regulates cell surface growth, bud formation and
CC morphogenesis. May act indirectly by regulating factor(s) involved in
CC these processes. {ECO:0000269|PubMed:8582632}.
CC -!- INTERACTION:
CC P36146; Q07845: GRC3; NbExp=5; IntAct=EBI-10053, EBI-3741923;
CC -!- SUBCELLULAR LOCATION: Nucleus. Mitochondrion.
CC -!- MISCELLANEOUS: Present with 647 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the LAS1 family. {ECO:0000305}.
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DR EMBL; U09670; AAC49111.1; -; Genomic_DNA.
DR EMBL; Z28288; CAA82142.1; -; Genomic_DNA.
DR EMBL; AY723842; AAU09759.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09214.1; -; Genomic_DNA.
DR PIR; S38139; S38139.
DR RefSeq; NP_012989.3; NM_001179853.3.
DR AlphaFoldDB; P36146; -.
DR SMR; P36146; -.
DR BioGRID; 34194; 240.
DR ComplexPortal; CPX-3384; LAS1 RNA processome complex.
DR DIP; DIP-6398N; -.
DR IntAct; P36146; 3.
DR STRING; 4932.YKR063C; -.
DR iPTMnet; P36146; -.
DR MaxQB; P36146; -.
DR PaxDb; P36146; -.
DR PRIDE; P36146; -.
DR EnsemblFungi; YKR063C_mRNA; YKR063C; YKR063C.
DR GeneID; 853937; -.
DR KEGG; sce:YKR063C; -.
DR SGD; S000001771; LAS1.
DR VEuPathDB; FungiDB:YKR063C; -.
DR eggNOG; KOG2425; Eukaryota.
DR GeneTree; ENSGT00390000014785; -.
DR HOGENOM; CLU_043823_0_0_1; -.
DR InParanoid; P36146; -.
DR OMA; HWGTHER; -.
DR BioCyc; YEAST:G3O-32031-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:P36146; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36146; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0090730; C:Las1 complex; IPI:ComplexPortal.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IPI:SGD.
DR GO; GO:0004519; F:endonuclease activity; IDA:SGD.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IDA:SGD.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IMP:SGD.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IMP:SGD.
DR InterPro; IPR007174; Las1.
DR PANTHER; PTHR15002; PTHR15002; 1.
DR Pfam; PF04031; Las1; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Nucleus; Reference proteome.
FT CHAIN 1..502
FT /note="Protein LAS1"
FT /id="PRO_0000211561"
FT REGION 469..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 97
FT /note="N -> D (in Ref. 4; AAU09759)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 502 AA; 59351 MW; B69F14A3DE211FB8 CRC64;
MIPPRIVPWR DFAELEELKL WFYPKSKGTI EDKRQRAVQR VQSYRLKGSQ YLPHVVDSTA
QITCAVLLDE KEACLGVHQD SIPIRLSYVM ALIRFVNGLL DPTQQSQFAI PLHTLAAKIG
LPSWFVDLRH WGTHERDLPG LEMLRWAANE ALSWLYDHYW NDEELEDDRD DDDDDDDTGY
GYRRNDKLEK YMESLTKTLD KWKRLRNEFL EYKWVWENAN DSLITSSNFS GDNLVNYDAE
KRKSSHASSS ETMIRENLRQ WQELWKLSIY HNVVLEKFFN NYDPLLLKVL MLNLNNFDWK
VIEWVARNYR TQQDDSNITT ILKRKFNAWK ELQKRLLDVI INNLNNKNFK NKWQNWEKLI
DENASYLILY FCQSMLAKLE TEKITGNSWR NKKRRKQIDS TVEIEAKLKE NIDNLSLRFN
EGEIKLYDFI PAEKDSVPLK KEVSPALKAD TNDILGDLAS LKQRMSSFGT VGKKNKQEEN
RATPVKNWSR VQNWKPKPFG VL
