LASR_PSEAE
ID LASR_PSEAE Reviewed; 239 AA.
AC P25084; Q51475; Q51476;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcriptional activator protein LasR;
GN Name=lasR; OrderedLocusNames=PA1430;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=1902216; DOI=10.1128/jb.173.9.3000-3009.1991;
RA Gambello M.J., Iglewski B.H.;
RT "Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a
RT transcriptional activator of elastase expression.";
RL J. Bacteriol. 173:3000-3009(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29260 / PA103, and NBRC 13130 / JCM 20134;
RX PubMed=7937080; DOI=10.1093/nar/22.18.3706;
RA Fukushima J., Ishiwata T., Kurata M., You Z., Okuda K.;
RT "Intracellular receptor-type transcription factor, LasR, contains a highly
RT conserved amphipathic region which precedes the putative helix-turn-helix
RT DNA binding motif.";
RL Nucleic Acids Res. 22:3706-3707(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Transcriptional activator of elastase structural gene (LasB).
CC Binds to the PAI autoinducer.
CC -!- MISCELLANEOUS: LasR in strain PA103 is not active, this is probably due
CC to the change in position 180 of the sequence.
CC -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional
CC regulatory protein family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M59425; AAA25874.1; -; Genomic_DNA.
DR EMBL; D30813; BAA06490.1; -; Genomic_DNA.
DR EMBL; D30812; BAA06489.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04819.1; -; Genomic_DNA.
DR PIR; A43660; A43660.
DR PIR; S53692; S53692.
DR PIR; S53693; S53693.
DR RefSeq; NP_250121.1; NC_002516.2.
DR RefSeq; WP_003082999.1; NZ_QZGE01000005.1.
DR PDB; 2UV0; X-ray; 1.80 A; E/F/G/H=1-173.
DR PDB; 3IX3; X-ray; 1.40 A; A/B=1-173.
DR PDB; 3IX4; X-ray; 1.80 A; A/B/C/D/E/F/G/H=1-173.
DR PDB; 3IX8; X-ray; 1.80 A; A/B/C/D=1-173.
DR PDB; 3JPU; X-ray; 2.30 A; A/B/C/D/E=1-173.
DR PDB; 4NG2; X-ray; 2.41 A; A/B/C/D=1-170.
DR PDB; 6D6A; X-ray; 1.90 A; A/B/C/D/E/F/G/H=1-170.
DR PDB; 6D6B; X-ray; 1.70 A; A/B/C/D/E/F/G/H=1-170.
DR PDB; 6D6C; X-ray; 1.88 A; A/B/C/D/E/F/G/H/I/J/K/L=1-170.
DR PDB; 6D6D; X-ray; 1.70 A; A/B=1-169.
DR PDB; 6D6L; X-ray; 1.63 A; A/B/C/D=1-170.
DR PDB; 6D6M; X-ray; 1.90 A; A/B/C/D=1-170.
DR PDB; 6D6N; X-ray; 1.81 A; A/B/C/D=1-170.
DR PDB; 6D6O; X-ray; 1.65 A; A/B/C/D=1-170.
DR PDB; 6D6P; X-ray; 1.65 A; A/B/C/D=1-170.
DR PDB; 6MWH; X-ray; 2.20 A; A/B=1-239.
DR PDB; 6MWL; X-ray; 1.50 A; A/B=1-239.
DR PDB; 6MWW; X-ray; 2.76 A; A/B=1-239.
DR PDB; 6MWZ; X-ray; 1.66 A; A/B=1-239.
DR PDB; 6V7W; X-ray; 3.00 A; B/E=1-239.
DR PDB; 6V7X; X-ray; 2.90 A; B=1-239.
DR PDBsum; 2UV0; -.
DR PDBsum; 3IX3; -.
DR PDBsum; 3IX4; -.
DR PDBsum; 3IX8; -.
DR PDBsum; 3JPU; -.
DR PDBsum; 4NG2; -.
DR PDBsum; 6D6A; -.
DR PDBsum; 6D6B; -.
DR PDBsum; 6D6C; -.
DR PDBsum; 6D6D; -.
DR PDBsum; 6D6L; -.
DR PDBsum; 6D6M; -.
DR PDBsum; 6D6N; -.
DR PDBsum; 6D6O; -.
DR PDBsum; 6D6P; -.
DR PDBsum; 6MWH; -.
DR PDBsum; 6MWL; -.
DR PDBsum; 6MWW; -.
DR PDBsum; 6MWZ; -.
DR PDBsum; 6V7W; -.
DR PDBsum; 6V7X; -.
DR AlphaFoldDB; P25084; -.
DR BMRB; P25084; -.
DR SMR; P25084; -.
DR STRING; 287.DR97_592; -.
DR BindingDB; P25084; -.
DR ChEMBL; CHEMBL1075207; -.
DR DrugBank; DB08324; N-3-OXO-DODECANOYL-L-HOMOSERINE LACTONE.
DR PaxDb; P25084; -.
DR PRIDE; P25084; -.
DR DNASU; 881789; -.
DR EnsemblBacteria; AAG04819; AAG04819; PA1430.
DR GeneID; 881789; -.
DR KEGG; pae:PA1430; -.
DR PATRIC; fig|208964.12.peg.1480; -.
DR PseudoCAP; PA1430; -.
DR HOGENOM; CLU_072786_6_0_6; -.
DR InParanoid; P25084; -.
DR OMA; RESLQWV; -.
DR PhylomeDB; P25084; -.
DR BioCyc; PAER208964:G1FZ6-1456-MON; -.
DR EvolutionaryTrace; P25084; -.
DR PHI-base; PHI:5183; -.
DR PHI-base; PHI:7655; -.
DR PHI-base; PHI:8640; -.
DR PHI-base; PHI:8641; -.
DR PHI-base; PHI:8821; -.
DR PRO; PR:P25084; -.
DR Proteomes; UP000002438; Chromosome.
DR CollecTF; EXPREG_000009b0; -.
DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:PseudoCAP.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:CollecTF.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR GO; GO:0051544; P:positive regulation of elastin biosynthetic process; IMP:PseudoCAP.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:CACAO.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR GO; GO:0060310; P:regulation of elastin catabolic process; IMP:PseudoCAP.
DR GO; GO:0010468; P:regulation of gene expression; IDA:CACAO.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:PseudoCAP.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.450.80; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03472; Autoind_bind; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF75516; SSF75516; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Quorum sensing; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..239
FT /note="Transcriptional activator protein LasR"
FT /id="PRO_0000184160"
FT DOMAIN 170..235
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 194..213
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT VARIANT 144
FT /note="M -> I (in strain: IFO 3455 and PA103)"
FT VARIANT 180
FT /note="R -> W (in strain: PA103)"
FT HELIX 8..12
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 15..29
FT /evidence="ECO:0007829|PDB:3IX3"
FT STRAND 33..40
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 47..49
FT /evidence="ECO:0007829|PDB:3IX3"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 58..66
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 69..71
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 74..81
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 90..92
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 96..107
FT /evidence="ECO:0007829|PDB:3IX3"
FT STRAND 112..119
FT /evidence="ECO:0007829|PDB:3IX3"
FT STRAND 125..131
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 137..145
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 148..167
FT /evidence="ECO:0007829|PDB:3IX3"
FT HELIX 179..189
FT /evidence="ECO:0007829|PDB:6V7X"
FT HELIX 194..201
FT /evidence="ECO:0007829|PDB:6V7X"
FT HELIX 205..219
FT /evidence="ECO:0007829|PDB:6V7X"
FT HELIX 224..233
FT /evidence="ECO:0007829|PDB:6V7X"
SQ SEQUENCE 239 AA; 26619 MW; 28B6E06E9187924A CRC64;
MALVDGFLEL ERSSGKLEWS AILQKMASDL GFSKILFGLL PKDSQDYENA FIVGNYPAAW
REHYDRAGYA RVDPTVSHCT QSVLPIFWEP SIYQTRKQHE FFEEASAAGL VYGLTMPLHG
ARGELGALSL SVEAENRAEA NRFMESVLPT LWMLKDYALQ SGAGLAFEHP VSKPVVLTSR
EKEVLQWCAI GKTSWEISVI CNCSEANVNF HMGNIRRKFG VTSRRVAAIM AVNLGLITL
