LAT2B_LACTA
ID LAT2B_LACTA Reviewed; 84 AA.
AC Q1ELU0;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=M-zodatoxin-Lt2b {ECO:0000305};
DE Short=M-ZDTX-Lt2b {ECO:0000305};
DE AltName: Full=Latarcin-2b {ECO:0000303|PubMed:16735513};
DE Short=Ltc-2b {ECO:0000303|PubMed:16735513};
DE Flags: Precursor;
OS Lachesana tarabaevi (Spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC Lachesana.
OX NCBI_TaxID=379576;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=16735513; DOI=10.1074/jbc.m602168200;
RA Kozlov S.A., Vassilevski A.A., Feofanov A.V., Surovoy A.Y., Karpunin D.V.,
RA Grishin E.V.;
RT "Latarcins, antimicrobial and cytolytic peptides from the venom of the
RT spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular
RT diversity.";
RL J. Biol. Chem. 281:20983-20992(2006).
RN [2]
RP PROTEIN SEQUENCE OF 59-84, SUBCELLULAR LOCATION, PQM MOTIF, AND MASS
RP SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=27287558; DOI=10.1042/bcj20160436;
RA Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA Vassilevski A.A.;
RT "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL Biochem. J. 473:2495-2506(2016).
CC -!- FUNCTION: Has antimicrobial activity against both Gram-positive and
CC Gram-negative bacteria, and yeasts. Also has a strong hemolytic
CC activity against rabbit erythrocytes. Causes paralysis, but is not
CC lethal when injected into insect (M.domestica) larvae (By similarity).
CC {ECO:0000250|UniProtKB:Q1ELU1}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27287558}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:27287558}.
CC -!- DOMAIN: The mature peptide (59-84) forms alpha-helices which probably
CC disrupt target cell membranes. {ECO:0000250|UniProtKB:Q1ELU7}.
CC -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC motif (XXXR, with at least one of X being E).
CC {ECO:0000303|PubMed:27287558}.
CC -!- MASS SPECTROMETRY: Mass=2879.7; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:27287558};
CC -!- SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 02
CC subfamily. {ECO:0000305}.
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DR EMBL; AM232699; CAJ81659.1; -; mRNA.
DR AlphaFoldDB; Q1ELU0; -.
DR SMR; Q1ELU0; -.
DR ArachnoServer; AS000051; M-zodatoxin-Lt2b.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR018802; Latarcin_precursor.
DR Pfam; PF10279; Latarcin; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Fungicide;
KW Hemolysis; Secreted; Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..58
FT /evidence="ECO:0000250"
FT /id="PRO_0000249738"
FT PEPTIDE 59..84
FT /note="M-zodatoxin-Lt2b"
FT /id="PRO_0000249739"
FT MOTIF 55..58
FT /note="Processing quadruplet motif"
FT /evidence="ECO:0000303|PubMed:27287558"
SQ SEQUENCE 84 AA; 9341 MW; B25A4D69A520E76F CRC64;
MKYFVIALAL AVALVCIAES TAYEVNEELE NELDDLDDAA WLAVAEELQG LEDFEESRGL
FGKLIKKFGR KAISYAVKKA RGKN
