LAT5_LACTA
ID LAT5_LACTA Reviewed; 93 AA.
AC Q1ELU9;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=M-zodatoxin-Lt5a {ECO:0000305};
DE Short=M-ZDTX-Lt5a {ECO:0000305};
DE AltName: Full=Latarcin-5 {ECO:0000303|PubMed:16735513};
DE Short=Ltc-5 {ECO:0000303|PubMed:16735513};
DE Flags: Precursor;
OS Lachesana tarabaevi (Spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC Lachesana.
OX NCBI_TaxID=379576;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 65-92, SYNTHESIS OF 65-92,
RP AMIDATION AT LEU-92, FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MASS
RP SPECTROMETRY.
RC TISSUE=Venom, and Venom gland;
RX PubMed=16735513; DOI=10.1074/jbc.m602168200;
RA Kozlov S.A., Vassilevski A.A., Feofanov A.V., Surovoy A.Y., Karpunin D.V.,
RA Grishin E.V.;
RT "Latarcins, antimicrobial and cytolytic peptides from the venom of the
RT spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular
RT diversity.";
RL J. Biol. Chem. 281:20983-20992(2006).
RN [2]
RP SUBCELLULAR LOCATION, PQM MOTIF, MASS SPECTROMETRY, AND AMIDATION AT
RP LEU-92.
RC TISSUE=Venom;
RX PubMed=27287558; DOI=10.1042/bcj20160436;
RA Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA Vassilevski A.A.;
RT "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL Biochem. J. 473:2495-2506(2016).
CC -!- FUNCTION: Has antimicrobial activity against. Gram-positive bacteria
CC (A.globiformis VKM Ac-1112 (MIC=1.1 uM), and B.subtilis VKM B-501
CC (MIC=0.6 uM)), Gram-negative bacteria (E.coli DH5-alpha (MIC=0.6 uM),
CC E.coli MH1 (MIC=0.6 uM), and P.aeruginosa PAO1 (MIC=18 uM)), and yeasts
CC (P.pastoris GS115 (MIC>37 uM), and S.cerevisiae Y190 (MIC>37 uM)). Also
CC has a moderate hemolytic activity against rabbit erythrocytes. Causes
CC paralysis, but is not lethal when injected into insect (M.domestica)
CC larvae. {ECO:0000269|PubMed:16735513}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16735513,
CC ECO:0000269|PubMed:27287558}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:16735513, ECO:0000305|PubMed:27287558}.
CC -!- DOMAIN: The mature peptide (65-92) probably forms alpha-helices which
CC disrupt target cell membranes. {ECO:0000303|PubMed:16735513}.
CC -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC motif (XXXR, with at least one of X being E).
CC {ECO:0000303|PubMed:27287558}.
CC -!- MASS SPECTROMETRY: Mass=3428.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:16735513};
CC -!- MASS SPECTROMETRY: Mass=3430.4; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:27287558};
CC -!- SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 05
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM232690; CAJ81650.1; -; mRNA.
DR AlphaFoldDB; Q1ELU9; -.
DR ArachnoServer; AS000056; M-zodatoxin-Lt5a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR018802; Latarcin_precursor.
DR Pfam; PF10279; Latarcin; 1.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW Fungicide; Hemolysis; Secreted; Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..64
FT /evidence="ECO:0000269|PubMed:16735513"
FT /id="PRO_0000249748"
FT PEPTIDE 65..92
FT /note="M-zodatoxin-Lt5a"
FT /id="PRO_0000249749"
FT MOTIF 61..64
FT /note="Processing quadruplet motif"
FT /evidence="ECO:0000303|PubMed:27287558"
FT MOD_RES 92
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:16735513,
FT ECO:0000269|PubMed:27287558"
SQ SEQUENCE 93 AA; 10632 MW; ED219757E807805F CRC64;
MKYCVVILAL LVALVCITES RSTETGYAVA ETLEDNDLDE LQAYLEEIAE ASEMEDFSNI
EEARGFFGKM KEYFKKFGAS FKRRFANLKK RLG
