ID   LAT61_LACTA             Reviewed;         116 AA.
AC   Q1ELU8;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=M-zodatoxin-Lt6a/b {ECO:0000305};
DE            Short=M-ZDTX-Lt6a/b {ECO:0000305};
DE   AltName: Full=Latarcin 6-1 {ECO:0000303|PubMed:16735513};
DE            Short=Ltc 6-1 {ECO:0000303|PubMed:16735513};
DE   Contains:
DE     RecName: Full=M-zodatoxin-Lt6b {ECO:0000305};
DE              Short=M-ZDTX-Lt6b {ECO:0000305};
DE     AltName: Full=Latarcin-6b;
DE              Short=Ltc-6b;
DE   Contains:
DE     RecName: Full=M-zodatoxin-Lt6a {ECO:0000305};
DE              Short=M-ZDTX-Lt6a {ECO:0000305};
DE     AltName: Full=Latarcin-6a {ECO:0000303|PubMed:27287558};
DE              Short=Ltc-6a {ECO:0000303|PubMed:27287558};
DE   Flags: Precursor;
OS   Lachesana tarabaevi (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC   Lachesana.
OX   NCBI_TaxID=379576;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 84-116, FUNCTION, SUBCELLULAR
RP   LOCATION, AND DOMAIN.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=16735513; DOI=10.1074/jbc.m602168200;
RA   Kozlov S.A., Vassilevski A.A., Feofanov A.V., Surovoy A.Y., Karpunin D.V.,
RA   Grishin E.V.;
RT   "Latarcins, antimicrobial and cytolytic peptides from the venom of the
RT   spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular
RT   diversity.";
RL   J. Biol. Chem. 281:20983-20992(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 84-116, SUBCELLULAR LOCATION, PQM MOTIF, MASS
RP   SPECTROMETRY, AND PYROGLUTAMATE FORMATION AT GLN-84.
RC   TISSUE=Venom;
RX   PubMed=27287558; DOI=10.1042/bcj20160436;
RA   Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA   Vassilevski A.A.;
RT   "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL   Biochem. J. 473:2495-2506(2016).
CC   -!- FUNCTION: [M-zodatoxin-Lt6a]: Does not have antimicrobial activity
CC       against neither Gram-positive bacteria (A.globiformis VKM Ac-1112
CC       (MIC>70 uM), and B.subtilis VKM B-501 (MIC>70 uM)), nor Gram-negative
CC       bacteria (E.coli DH5-alpha (MIC>70 uM), E.coli MH1 (MIC>70 uM), and
CC       P.aeruginosa PAO1 (MIC>70 uM)), nor yeasts (P.pastoris GS115 (MIC>70
CC       uM), and S.cerevisiae Y190 (MIC>70 uM)). Does not have hemolytic
CC       activity against rabbit erythrocytes. However, it causes some
CC       conductance changes in planar bilayer membranes, without membrane
CC       rupture, suggesting a cytolytic function on other biological targets.
CC       It causes paralysis, but is not lethal when injected into insect
CC       (M.domestica) larvae. {ECO:0000269|PubMed:16735513}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16735513,
CC       ECO:0000269|PubMed:27287558}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:16735513, ECO:0000305|PubMed:27287558}.
CC   -!- DOMAIN: The mature peptides (45-79 and 84-116) probably form alpha-
CC       helices which disrupt target cell membranes.
CC       {ECO:0000303|PubMed:16735513}.
CC   -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC       motif (XXXR, with at least one of X being E).
CC       {ECO:0000303|PubMed:27287558}.
CC   -!- MASS SPECTROMETRY: [M-zodatoxin-Lt6a]: Mass=4035.1; Method=MALDI;
CC       Note=M-zodatoxin-Lt6a.; Evidence={ECO:0000269|PubMed:27287558};
CC   -!- SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 06
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AM232691; CAJ81651.1; -; mRNA.
DR   AlphaFoldDB; Q1ELU8; -.
DR   SMR; Q1ELU8; -.
DR   ArachnoServer; AS000057; M-zodatoxin-Lt6a.
DR   ArachnoServer; AS000762; M-zodatoxin-Lt6b.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR018802; Latarcin_precursor.
DR   Pfam; PF10279; Latarcin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Pyrrolidone carboxylic acid; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..44
FT                   /id="PRO_0000249750"
FT   PEPTIDE         45..79
FT                   /note="M-zodatoxin-Lt6b"
FT                   /id="PRO_0000249751"
FT   PROPEP          80..83
FT                   /id="PRO_0000249752"
FT   PEPTIDE         84..116
FT                   /note="M-zodatoxin-Lt6a"
FT                   /evidence="ECO:0000269|PubMed:27287558"
FT                   /id="PRO_0000249753"
FT   MOTIF           41..44
FT                   /note="Processing quadruplet motif 1"
FT                   /evidence="ECO:0000303|PubMed:27287558"
FT   MOTIF           80..83
FT                   /note="Processing quadruplet motif 2"
FT                   /evidence="ECO:0000303|PubMed:27287558"
FT   MOD_RES         84
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:27287558"
SQ   SEQUENCE   116 AA;  13846 MW;  9B0A0A0A36D8C538 CRC64;
     MKYFVVALTL AVAFVCIEEC KTVEIGYAVS EDFDQNEIDN EEARQAFKTF TPDWNKIRND
     AKRMQDNLEQ MKKRFNLNLE EARQAFQTFK PDWNKIRYDA MKMQTSLGQM KKRFNL