LAT62_LACTA
ID LAT62_LACTA Reviewed; 116 AA.
AC Q1ELU7;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=M-zodatoxin-Lt6a/c {ECO:0000305};
DE Short=M-ZDTX-Lt6a/c {ECO:0000305};
DE AltName: Full=Latarcin 6-2 {ECO:0000303|PubMed:16735513};
DE Short=Ltc 6-2 {ECO:0000303|PubMed:16735513};
DE Contains:
DE RecName: Full=M-zodatoxin-Lt6c {ECO:0000305};
DE Short=M-ZDTX-Lt6c {ECO:0000305};
DE AltName: Full=Latarcin-6c;
DE Short=Ltc-6c;
DE Contains:
DE RecName: Full=M-zodatoxin-Lt6a {ECO:0000305};
DE Short=M-ZDTX-Lt6a {ECO:0000305};
DE AltName: Full=Latarcin-6a {ECO:0000303|PubMed:27287558};
DE Short=Ltc-6a {ECO:0000303|PubMed:27287558};
DE Flags: Precursor;
OS Lachesana tarabaevi (Spider).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC Lachesana.
OX NCBI_TaxID=379576;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 84-116, FUNCTION, SUBCELLULAR
RP LOCATION, AND DOMAIN.
RC TISSUE=Venom, and Venom gland;
RX PubMed=16735513; DOI=10.1074/jbc.m602168200;
RA Kozlov S.A., Vassilevski A.A., Feofanov A.V., Surovoy A.Y., Karpunin D.V.,
RA Grishin E.V.;
RT "Latarcins, antimicrobial and cytolytic peptides from the venom of the
RT spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular
RT diversity.";
RL J. Biol. Chem. 281:20983-20992(2006).
RN [2]
RP PROTEIN SEQUENCE OF 84-116, SUBCELLULAR LOCATION, PQM MOTIF, MASS
RP SPECTROMETRY, AND PYROGLUTAMATE FORMATION AT GLN-84.
RC TISSUE=Venom;
RX PubMed=27287558; DOI=10.1042/bcj20160436;
RA Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA Vassilevski A.A.;
RT "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL Biochem. J. 473:2495-2506(2016).
CC -!- FUNCTION: [M-zodatoxin-Lt6a]: Does not have antimicrobial activity
CC against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC>70 uM)
CC and B.subtilis VKM B-501 (MIC>70 uM)), Gram-negative bacteria (E.coli
CC DH5-alpha (MIC>70 uM), E.coli MH1 (MIC>70 uM) and P.aeruginosa PAO1
CC (MIC>70 uM)), yeast (P.pastoris GS115 (MIC>70 uM) or S.cerevisiae Y190
CC (MIC>70 uM)). Does not have hemolytic activity against rabbit
CC erythrocytes. However, it causes some conductance changes in planar
CC bilayer membranes, without membrane rupture, suggesting a cytolytic
CC function on other biological targets. It causes paralysis, but is not
CC lethal when injected into insect larvae. {ECO:0000269|PubMed:16735513}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16735513,
CC ECO:0000269|PubMed:27287558}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:16735513, ECO:0000305|PubMed:27287558}.
CC -!- DOMAIN: The mature peptides (45-79 and 84-116) probably form alpha-
CC helices which disrupt target cell membranes.
CC {ECO:0000303|PubMed:16735513}.
CC -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC motif (XXXR, with at least one of X being E).
CC {ECO:0000303|PubMed:27287558}.
CC -!- MASS SPECTROMETRY: [M-zodatoxin-Lt6a]: Mass=4035.1; Method=MALDI;
CC Note=M-zodatoxin-Lt6a.; Evidence={ECO:0000269|PubMed:27287558};
CC -!- SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 06
CC subfamily. {ECO:0000305}.
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DR EMBL; AM232692; CAJ81652.1; -; mRNA.
DR AlphaFoldDB; Q1ELU7; -.
DR SMR; Q1ELU7; -.
DR ArachnoServer; AS000057; M-zodatoxin-Lt6a.
DR ArachnoServer; AS000058; M-zodatoxin-Lt6c.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR018802; Latarcin_precursor.
DR Pfam; PF10279; Latarcin; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Pyrrolidone carboxylic acid; Secreted; Signal;
KW Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..44
FT /id="PRO_0000249754"
FT PEPTIDE 45..79
FT /note="M-zodatoxin-Lt6c"
FT /id="PRO_0000249755"
FT PROPEP 80..83
FT /id="PRO_0000249756"
FT PEPTIDE 84..116
FT /note="M-zodatoxin-Lt6a"
FT /evidence="ECO:0000269|PubMed:27287558"
FT /id="PRO_0000249757"
FT MOTIF 41..44
FT /note="Processing quadruplet motif 1"
FT /evidence="ECO:0000303|PubMed:27287558"
FT MOTIF 80..83
FT /note="Processing quadruplet motif 2"
FT /evidence="ECO:0000303|PubMed:27287558"
FT MOD_RES 84
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:27287558"
SQ SEQUENCE 116 AA; 13804 MW; F07A14C44E70C47C CRC64;
MKYFVVALTL AVAFVCIEEC KTVEIGYAVS EDFDQNEIDN DEARQAFKTF TPDWNKIRND
AKRMQDNLEQ MKKKFNLNLE EARQAFQTFK PDWNKIRYDA MKMQTSLGQM KKRFNL
