ID   LAT7_LACTA              Reviewed;          97 AA.
AC   Q1ELV0;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=M-zodatoxin-Lt7a {ECO:0000305};
DE            Short=M-ZDTX-Lt7a {ECO:0000305};
DE   AltName: Full=Latarcin-7 {ECO:0000303|PubMed:16735513};
DE            Short=Ltc-7 {ECO:0000303|PubMed:16735513};
DE   Flags: Precursor;
OS   Lachesana tarabaevi (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Entelegynae incertae sedis; Zodariidae;
OC   Lachesana.
OX   NCBI_TaxID=379576;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 64-97, FUNCTION, SUBCELLULAR
RP   LOCATION, AND DOMAIN.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=16735513; DOI=10.1074/jbc.m602168200;
RA   Kozlov S.A., Vassilevski A.A., Feofanov A.V., Surovoy A.Y., Karpunin D.V.,
RA   Grishin E.V.;
RT   "Latarcins, antimicrobial and cytolytic peptides from the venom of the
RT   spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular
RT   diversity.";
RL   J. Biol. Chem. 281:20983-20992(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 64-97, SUBCELLULAR LOCATION, PQM MOTIF, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=27287558; DOI=10.1042/bcj20160436;
RA   Kuzmenkov A.I., Sachkova M.Y., Kovalchuk S.I., Grishin E.V.,
RA   Vassilevski A.A.;
RT   "Lachesana tarabaevi, an expert in membrane-active toxins.";
RL   Biochem. J. 473:2495-2506(2016).
CC   -!- FUNCTION: Does not have antimicrobial or antifungal activity. Does not
CC       have hemolytic activity against rabbit erythrocytes. However, it causes
CC       some conductance changes in planar bilayer membranes, without membrane
CC       rupture, suggesting a cytolytic function on other biological targets.
CC       It causes paralysis, but is not lethal when injected into insect
CC       (M.domestica) larvae. {ECO:0000269|PubMed:16735513}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16735513,
CC       ECO:0000269|PubMed:27287558}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:16735513}.
CC   -!- DOMAIN: The mature peptide (64-97) probably forms alpha-helices which
CC       disrupt target cell membranes. {ECO:0000303|PubMed:16735513}.
CC   -!- PTM: Cleavage of the propeptide depends on the processing quadruplet
CC       motif (XXXR, with at least one of X being E).
CC       {ECO:0000303|PubMed:27287558}.
CC   -!- MASS SPECTROMETRY: Mass=3942.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27287558};
CC   -!- SIMILARITY: Belongs to the cationic peptide 03 (latarcin) family. 07
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AM232689; CAJ81648.1; -; mRNA.
DR   AlphaFoldDB; Q1ELV0; -.
DR   SMR; Q1ELV0; -.
DR   ArachnoServer; AS000059; M-zodatoxin-Lt7a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR018802; Latarcin_precursor.
DR   Pfam; PF10279; Latarcin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..63
FT                   /id="PRO_0000249758"
FT   PEPTIDE         64..97
FT                   /note="M-zodatoxin-Lt7a"
FT                   /evidence="ECO:0000269|PubMed:27287558"
FT                   /id="PRO_0000249759"
FT   MOTIF           60..63
FT                   /note="Processing quadruplet motif"
FT                   /evidence="ECO:0000303|PubMed:27287558"
SQ   SEQUENCE   97 AA;  11021 MW;  93FFC5CB6C98DD25 CRC64;
     MKFYVVALAL LVAFVCIAES RSVETERAVD ADLEDDLDDL EEYLEGIAEA LELEDFPDTE
     EARGETFDKL KEKLKTFYQK LVEKAEDLKG DLKAKLS