LAV1_PHYPO
ID LAV1_PHYPO Reviewed; 355 AA.
AC P14725;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Plasmodial-specific protein LAV1-2;
OS Physarum polycephalum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Myxogastria;
OC Myxogastromycetidae; Physariida; Physaraceae; Physarum.
OX NCBI_TaxID=5791;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=M3C;
RX PubMed=2602164; DOI=10.1093/nar/17.24.10502;
RA Laroche A., Lemieux G., Pallotta D.;
RT "The nucleotide sequence of a developmentally regulated cDNA from Physarum
RT polycephalum.";
RL Nucleic Acids Res. 17:10502-10502(1989).
CC -!- MISCELLANEOUS: This protein has four EF-hand homolog domains inferred
CC to bind calcium.
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DR EMBL; X14502; CAA32655.1; -; mRNA.
DR PIR; S06939; S06939.
DR PDB; 1IJ5; X-ray; 3.00 A; A=33-355.
DR PDB; 1IJ6; X-ray; 3.10 A; A=33-355.
DR PDBsum; 1IJ5; -.
DR PDBsum; 1IJ6; -.
DR AlphaFoldDB; P14725; -.
DR SMR; P14725; -.
DR PRIDE; P14725; -.
DR EvolutionaryTrace; P14725; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 1.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Metal-binding; Repeat.
FT CHAIN 1..355
FT /note="Plasmodial-specific protein LAV1-2"
FT /id="PRO_0000073742"
FT DOMAIN 151..186
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 217..252
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 282..317
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 319..354
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 230
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 236
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 241
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 265
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 267
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 269
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 271
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 276
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 295
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 297
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 299
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 301
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 306
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 332
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 334
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 336
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 338
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 343
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT HELIX 37..60
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 74..112
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 117..130
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 150..160
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 162..165
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 173..184
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 191..201
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 203..205
FT /evidence="ECO:0007829|PDB:1IJ6"
FT HELIX 207..212
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 216..220
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 226..229
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 235..237
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 239..248
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 254..264
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 270..273
FT /evidence="ECO:0007829|PDB:1IJ6"
FT HELIX 274..293
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 296..298
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 304..313
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 318..320
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 324..331
FT /evidence="ECO:0007829|PDB:1IJ5"
FT TURN 332..334
FT /evidence="ECO:0007829|PDB:1IJ5"
FT STRAND 337..339
FT /evidence="ECO:0007829|PDB:1IJ5"
FT HELIX 341..351
FT /evidence="ECO:0007829|PDB:1IJ5"
SQ SEQUENCE 355 AA; 40504 MW; 18E1E5DD757AA413 CRC64;
MSYQEAWNPV DSSLDEIISI AASLKSKTGA VKEIFSQELT QREANVKKVH ENLEELQKKL
DHTSFAHKED RDRLEAQIAQ KEQEQKAKLA EYDQKVQNEF DARERAERER EAARGDAAAE
KQRLASLLKD LEDDASGYNR LRPSKPMLSE EDTNILRQLF LSSAVSGSGK FSFQDLKQVL
AKYADTIPEG PLKKLFVMVE NDTKGRMSYI TLVAVANDLA ALVADFRKID TNSNGTLSRK
EFREHFVRLG FDKKSVQDAL FRYADEDESD DVGFSEYVHL GLCLLVLRIL YAFADFDKSG
QLSKEEVQKV LEDAHIPESA RKKFEHQFSV VDVDDSKSLS YQEFVMLVLL MFHDD
