LAX12_ORYSJ
ID LAX12_ORYSJ Reviewed; 482 AA.
AC Q688J2; Q0DHR6;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Auxin transporter-like protein 2;
GN OrderedLocusNames=Os05g0447200, LOC_Os05g37470; ORFNames=OSJNBb0012G21.5;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx. May
CC mediate the formation of auxin gradient from developing leaves (site of
CC auxin biosynthesis) to tips (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q688J2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q688J2-2; Sequence=VSP_017012;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
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DR EMBL; AC135421; AAU10758.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17607.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS94291.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS94292.1; -; Genomic_DNA.
DR EMBL; AK111659; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK111849; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015637481.1; XM_015781995.1. [Q688J2-2]
DR RefSeq; XP_015637482.1; XM_015781996.1. [Q688J2-1]
DR AlphaFoldDB; Q688J2; -.
DR SMR; Q688J2; -.
DR STRING; 4530.OS05T0447200-01; -.
DR PaxDb; Q688J2; -.
DR PRIDE; Q688J2; -.
DR EnsemblPlants; Os05t0447200-01; Os05t0447200-01; Os05g0447200. [Q688J2-2]
DR EnsemblPlants; Os05t0447200-02; Os05t0447200-02; Os05g0447200. [Q688J2-1]
DR GeneID; 4338948; -.
DR Gramene; Os05t0447200-01; Os05t0447200-01; Os05g0447200. [Q688J2-2]
DR Gramene; Os05t0447200-02; Os05t0447200-02; Os05g0447200. [Q688J2-1]
DR KEGG; osa:4338948; -.
DR eggNOG; KOG1303; Eukaryota.
DR InParanoid; Q688J2; -.
DR OMA; HHTKSIF; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q688J2; baseline and differential.
DR Genevisible; Q688J2; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Auxin signaling pathway;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..482
FT /note="Auxin transporter-like protein 2"
FT /id="PRO_0000093851"
FT TOPO_DOM 1..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..78
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..313
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..355
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..423
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..482
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT VAR_SEQ 59
FT /note="V -> VRPTTNDLVMPLAHISFGILQV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_017012"
FT CONFLICT 446
FT /note="S -> G (in Ref. 5; AK111849)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 482 AA; 53358 MW; FAE0DBD64A686F79 CRC64;
MVPAGDQAEE AIVADAGKEE AEVRAAMGVE QDGKFSMTSL LWHGGSVWDA WFSCASNQVA
QVLLTLPYSF SQLGMLSGLL LQVFYGLMGS WTAYLISVLY VEYRARKEKE GVSFKNHVIQ
WFEVLDGLLG PYWKAAGLAF NCTFLLFGSV IQLIACASNI YYINDRLDKR TWTYIFGACC
STTVFIPSFH NYRIWSFLGL GMTTYTAWYL AIAAAVHGQV DGVTHSGPSK MVLYFTGATN
ILYTFGGHAV TVEIMHAMWK PQKFKYIYLV ATLYVFTLTL PSASAMYWAF GDALLTHSNA
FSLLPRSGWR DAAVILMLIH QFITFGFACT PLYFVWEKAI GMHGTRSVLT RALARLPIVV
PIWFLAIIFP FFGPINSAVG ALLVSFTVYI IPSLSHILTY RSASARLNAA EKPPPFLPSW
SGMFVVNVFV VAWVLVVGFG LGGWASVTNF IKQIDTFGLF AKCYQCPPRA HAGAPLPAPP
RH
