LAX1_ARATH
ID LAX1_ARATH Reviewed; 488 AA.
AC Q9LFB2; Q9SMW2;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Auxin transporter-like protein 1;
DE AltName: Full=AUX1-like protein 1;
GN Name=LAX1; OrderedLocusNames=At5g01240; ORFNames=F7J8_220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1007/s003440010030;
RA Parry P.G., Marchant A., May S.T., Swarup R., Swarup K., James N.,
RA Graham N., Allen T., Martucci T., Yemm A., Napier R., Manning K., King G.,
RA Bennett M.J.;
RT "Quick on the uptake: characterization of a family of plant auxin influx
RT carriers.";
RL J. Plant Growth Regul. 20:217-225(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LFB2-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ249442; CAB55758.1; -; mRNA.
DR EMBL; AL137189; CAB69852.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90315.1; -; Genomic_DNA.
DR PIR; T45964; T45964.
DR RefSeq; NP_195744.1; NM_120202.5. [Q9LFB2-1]
DR AlphaFoldDB; Q9LFB2; -.
DR BioGRID; 17142; 1.
DR STRING; 3702.AT5G01240.1; -.
DR iPTMnet; Q9LFB2; -.
DR PaxDb; Q9LFB2; -.
DR PRIDE; Q9LFB2; -.
DR ProteomicsDB; 237076; -. [Q9LFB2-1]
DR EnsemblPlants; AT5G01240.1; AT5G01240.1; AT5G01240. [Q9LFB2-1]
DR GeneID; 831866; -.
DR Gramene; AT5G01240.1; AT5G01240.1; AT5G01240. [Q9LFB2-1]
DR KEGG; ath:AT5G01240; -.
DR Araport; AT5G01240; -.
DR TAIR; locus:2150089; AT5G01240.
DR eggNOG; KOG1303; Eukaryota.
DR InParanoid; Q9LFB2; -.
DR OMA; FANCYQC; -.
DR OrthoDB; 509343at2759; -.
DR PhylomeDB; Q9LFB2; -.
DR PRO; PR:Q9LFB2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFB2; baseline and differential.
DR Genevisible; Q9LFB2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0010328; F:auxin influx transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048829; P:root cap development; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Auxin signaling pathway;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..488
FT /note="Auxin transporter-like protein 1"
FT /id="PRO_0000093842"
FT TOPO_DOM 1..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..84
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..141
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..202
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..238
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..320
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 342..362
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..427
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 449..488
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 303
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 16..17
FT /note="ED -> DE (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="V -> L (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
FT CONFLICT 365..366
FT /note="VV -> C (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
FT CONFLICT 372..373
FT /note="LA -> WP (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
FT CONFLICT 379..381
FT /note="FGP -> SA (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
FT CONFLICT 413..416
FT /note="RRNA -> AER (in Ref. 1; CAB55758)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 488 AA; 54601 MW; 06B0BF524EDE2BD8 CRC64;
MSGEKQAEES IVVSGEDEVA GRKVEDSAAE EDIDGNGGNG FSMKSFLWHG GSAWDAWFSC
ASNQVAQVLL TLPYSFSQLG MLSGILLQIF YGLMGSWTAY LISVLYVEYR ARMEKQEAKS
FKNHVIQWFE VLDGLLGPYW KAAGLAFNCT FLLFGSVIQL IACASNIYYI NDRLDKRTWT
YIFGACCATT VFIPSFHNYR IWSFLGLGMT TYTAWYLTIA SFLHGQAEGV THSGPTKLVL
YFTGATNILY TFGGHAVTVE IMHAMWKPRK FKSIYLMATL YVFTLTLPSA SAVYWAFGDQ
LLNHSNAFSL LPKTRFRDTA VILMLIHQFI TFGFACTPLY FVWEKAIGMH HTKSLCLRAL
VRLPVVVPIW FLAIIFPFFG PINSAVGALL VTFTVYIIPA LAHMLTYRTA SARRNAAEKP
PFFIPSWAGV YVINAFIVVW VLVLGFGFGG WASMTNFIRQ IDTFGLFAKC YQCKPPPAPI
AAGAHHRR
