LAX1_MEDTR
ID LAX1_MEDTR Reviewed; 479 AA.
AC Q9FEL8;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Auxin transporter-like protein 1;
DE AltName: Full=AUX1-like protein 1;
DE AltName: Full=MtLAX1;
GN Name=LAX1;
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jemalong; TISSUE=Root;
RX PubMed=11277424; DOI=10.1094/mpmi.2001.14.3.267;
RA de Billy F., Grosjean C., May S., Bennett M.J., Cullimore J.V.;
RT "Expression studies on AUX1-like genes in Medicago truncatula suggest that
RT auxin is required at two steps in early nodule development.";
RL Mol. Plant Microbe Interact. 14:267-277(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=15375694; DOI=10.1007/s00438-004-1057-x;
RA Schnabel E.L., Frugoli J.;
RT "The PIN and LAX families of auxin transport genes in Medicago
RT truncatula.";
RL Mol. Genet. Genomics 272:420-432(2004).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). May be involved in lateral roots and nodules formation.
CC {ECO:0000250, ECO:0000269|PubMed:11277424}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Shoots and roots of nodulating plants. Higher
CC levels in roots, flowers and stems, lower in nodules, leaves, petioles
CC and shoot apices. {ECO:0000269|PubMed:11277424,
CC ECO:0000269|PubMed:15375694}.
CC -!- DEVELOPMENTAL STAGE: In primary roots, mostly localized in tips and to
CC a lower extent in vasculature of older regions. In root tips, mostly
CC expressed in the central tissues of the elongating zone (developing
CC vasculature), in the starch-filled cells of the root cap and in some
CC cortical cells. During lateral root development, striking expression in
CC the proximal region of the primordium, close to the primary root
CC central cylinder, and then in elongating cells of the developing
CC vasculature and in developing root cap. During nodule formation,
CC expressed in young elongated primordium, mostly in cells close to the
CC root vasculature. In later stages, confined in small cells rich in
CC amyloplasts with small nuclei. Near the periphery of developing nodules
CC strong expression at the base that tapers off toward the apex. Not
CC expressed in mature nodules. {ECO:0000269|PubMed:11277424}.
CC -!- INDUCTION: Transient induction in roots by S.meliloti.
CC {ECO:0000269|PubMed:11277424}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: Because of the similarity in sequence, the probe used to
CC describe the developmental stages did not discriminate among the
CC various MtLAX mRNAs. {ECO:0000305}.
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DR EMBL; AJ299397; CAC12995.1; -; mRNA.
DR EMBL; AY115841; AAM55302.1; -; Genomic_DNA.
DR RefSeq; XP_003616599.1; XM_003616551.2.
DR AlphaFoldDB; Q9FEL8; -.
DR SMR; Q9FEL8; -.
DR STRING; 3880.AES99557; -.
DR EnsemblPlants; AES99557; AES99557; MTR_5g082220.
DR GeneID; 11406709; -.
DR Gramene; AES99557; AES99557; MTR_5g082220.
DR eggNOG; KOG1303; Eukaryota.
DR OMA; HHTKSIF; -.
DR OrthoDB; 509343at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Glycoprotein;
KW Membrane; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..479
FT /note="Auxin transporter-like protein 1"
FT /id="PRO_0000093845"
FT TOPO_DOM 1..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..78
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..195
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..313
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..355
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..479
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 474
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 54259 MW; 6887C4F5837F5A3B CRC64;
MLSEKQGEET MMSSLNETIE LNEEREEEKG ASPGSGFKNF LWHGGSVYDA WFSCASNQVA
QVLLTLPYSF SQLGMISGII FQVFYGLMGS WTAYLISILY VEYRSRKEKE NVSFKNHVIQ
WFEVLEGLLG PYWKAIGLAF NCTFLLFGSV IQLIACASNI YYINDHLDKR TWTYIFGACC
ATTVFIPSFH NYRIWSFLGL GMTTYTAWYM TIAAIVHGQV ENVVHSGPKK MVWYFTGATN
ILYTFGGHAV TVEIMHAMWK PQKFKAIYFF ATLYVFTLTL PSAIAVYWAF GDQLLDHSNA
FSLLPRNAWR DAGVILMLIH QFITFGFACT PLYFVWEKVI GMHDTKSIFL RALARLPVVI
PIWFLAIIFP FFGPINSAVG ALLVSFTVYV IPASAHMLTY RSASARQNAA EKLPKVIPSW
TLMYVINAFV VIWVTIVGFG FGGWASMTNF IKQVDTFGLF AKCYQCPPKL PASNHTMHH
