LAX1_ORYSJ
ID LAX1_ORYSJ Reviewed; 215 AA.
AC Q7XAQ6; Q0JI15;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Transcription factor LAX PANICLE 1 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 123 {ECO:0000303|PubMed:16896230};
DE Short=OsbHLH123 {ECO:0000303|PubMed:16896230};
DE AltName: Full=Protein LAX PANICLE {ECO:0000303|PubMed:13130077};
GN Name=LAX1 {ECO:0000303|PubMed:11237465};
GN Synonyms=BHLH123 {ECO:0000303|PubMed:16896230},
GN LAX {ECO:0000303|PubMed:13130077};
GN OrderedLocusNames=Os01g0831000 {ECO:0000312|EMBL:BAF06613.1},
GN LOC_Os01g61480 {ECO:0000305};
GN ORFNames=P0446G04.27 {ECO:0000312|EMBL:BAD81850.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND MUTAGENESIS OF ALA-49 AND ARG-50.
RC STRAIN=cv. Nipponbare;
RX PubMed=13130077; DOI=10.1073/pnas.1932414100;
RA Komatsu K., Maekawa M., Ujiie S., Satake Y., Furutani I., Okamoto H.,
RA Shimamoto K., Kyozuka J.;
RT "LAX and SPA: major regulators of shoot branching in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11765-11770(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kang S.G., Matin N.;
RT "Genetic and phenotypic functions of a lax panicle mutant gene in rice
RT (Oryza sativa L.).";
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP IDENTIFICATION OF LAX PANICLE DISRUPTION PHENOTYPE.
RX PubMed=11237465; DOI=10.1006/dbio.2000.9988;
RA Komatsu M., Maekawa M., Shimamoto K., Kyozuka J.;
RT "The LAX1 and FRIZZY PANICLE 2 genes determine the inflorescence
RT architecture of rice by controlling rachis-branch and spikelet
RT development.";
RL Dev. Biol. 231:364-373(2001).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [9]
RP INTERACTION WITH LAX2.
RC STRAIN=cv. Nipponbare;
RX PubMed=21963665; DOI=10.1105/tpc.111.088765;
RA Tabuchi H., Zhang Y., Hattori S., Omae M., Shimizu-Sato S., Oikawa T.,
RA Qian Q., Nishimura M., Kitano H., Xie H., Fang X., Yoshida H., Kyozuka J.,
RA Chen F., Sato Y.;
RT "LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the
RT formation of axillary meristems.";
RL Plant Cell 23:3276-3287(2011).
CC -!- FUNCTION: Transcription factor that seems to regulate organogenesis in
CC postembryonic development. Involved in the regulation of shoot
CC branching by controlling axillary meristem initiation. Functions in
CC association with LAX2 to regulate the process of AM formation.
CC Possesses transactivation activity in yeast (PubMed:21963665).
CC {ECO:0000269|PubMed:13130077, ECO:0000269|PubMed:21963665}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein (By similarity). Interacts with LAX2 (PubMed:21963665).
CC {ECO:0000250, ECO:0000269|PubMed:21963665}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in the boundary between the shoot apical
CC meristem (SAM) and the region of new meristem formation.
CC {ECO:0000269|PubMed:13130077}.
CC -!- DEVELOPMENTAL STAGE: Expressed in growing new meristem. Down-regulated
CC in elongating new meristem. {ECO:0000269|PubMed:13130077}.
CC -!- DISRUPTION PHENOTYPE: Defects in initiation and/or maintenance of
CC rachis-branches, lateral spikelets and terminal spikelets.
CC {ECO:0000269|PubMed:11237465}.
CC -!- SIMILARITY: Belongs to the bHLH protein family.
CC -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; AB115668; BAC80247.1; -; mRNA.
DR EMBL; JN210896; AEL31274.1; -; mRNA.
DR EMBL; AP003252; BAD81850.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06613.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75054.1; -; Genomic_DNA.
DR RefSeq; XP_015618581.1; XM_015763095.1.
DR AlphaFoldDB; Q7XAQ6; -.
DR SMR; Q7XAQ6; -.
DR STRING; 4530.OS01T0831000-01; -.
DR PaxDb; Q7XAQ6; -.
DR PRIDE; Q7XAQ6; -.
DR EnsemblPlants; Os01t0831000-01; Os01t0831000-01; Os01g0831000.
DR GeneID; 4327431; -.
DR Gramene; Os01t0831000-01; Os01t0831000-01; Os01g0831000.
DR KEGG; osa:4327431; -.
DR eggNOG; ENOG502S37F; Eukaryota.
DR HOGENOM; CLU_1216297_0_0_1; -.
DR InParanoid; Q7XAQ6; -.
DR OMA; MDPYHYQ; -.
DR OrthoDB; 1392607at2759; -.
DR PlantReactome; R-OSA-9608575; Reproductive meristem phase change.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q7XAQ6; OS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:2000032; P:regulation of secondary shoot formation; IMP:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..215
FT /note="Transcription factor LAX PANICLE 1"
FT /id="PRO_0000127260"
FT DOMAIN 40..89
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 40..53
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 54..89
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT MUTAGEN 49
FT /note="A->T: In lax-4; defects in lateral spikelets. Weak
FT phenotype."
FT /evidence="ECO:0000269|PubMed:13130077"
FT MUTAGEN 50
FT /note="R->D: In lax-5; defects in lateral spikelets. Weak
FT phenotype."
FT /evidence="ECO:0000269|PubMed:13130077"
SQ SEQUENCE 215 AA; 23092 MW; 9600D908C4B3773A CRC64;
MHDPRGFPIH PQPYHLHPTA GGLGEGRMRG GGRRRPGAKL STDPQSVAAR ERRHRISDRF
RVLRSLVPGG SKMDTVSMLE QAIHYVKFLK AQVTLHQAAL VQHEEGCQHA DVAAAFSAAD
ADLALELNHR HGGAGDDDAG MTTLEMAPMQ EAVGYGDGPA HQMMQQALDP AGQLMMGGAH
QLPPLPCCVF VQETDPSCYS VCNVHGEESG AQGSY
