LAX2_ARATH
ID LAX2_ARATH Reviewed; 483 AA.
AC Q9S836;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Auxin transporter-like protein 2;
DE AltName: Full=AUX1-like protein 2;
GN Name=LAX2; OrderedLocusNames=At2g21050; ORFNames=F26H11.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1007/s003440010030;
RA Parry P.G., Marchant A., May S.T., Swarup R., Swarup K., James N.,
RA Graham N., Allen T., Martucci T., Yemm A., Napier R., Manning K., King G.,
RA Bennett M.J.;
RT "Quick on the uptake: characterization of a family of plant auxin influx
RT carriers.";
RL J. Plant Growth Regul. 20:217-225(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ243221; CAB45643.1; -; Genomic_DNA.
DR EMBL; AC006264; AAD29811.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07116.1; -; Genomic_DNA.
DR EMBL; AY054684; AAK96875.1; -; mRNA.
DR EMBL; AY128714; AAM91114.1; -; mRNA.
DR PIR; E84596; E84596.
DR RefSeq; NP_179701.1; NM_127675.3.
DR AlphaFoldDB; Q9S836; -.
DR SMR; Q9S836; -.
DR BioGRID; 1993; 3.
DR IntAct; Q9S836; 1.
DR STRING; 3702.AT2G21050.1; -.
DR PaxDb; Q9S836; -.
DR PRIDE; Q9S836; -.
DR ProteomicsDB; 250717; -.
DR EnsemblPlants; AT2G21050.1; AT2G21050.1; AT2G21050.
DR GeneID; 816640; -.
DR Gramene; AT2G21050.1; AT2G21050.1; AT2G21050.
DR KEGG; ath:AT2G21050; -.
DR Araport; AT2G21050; -.
DR TAIR; locus:2047102; AT2G21050.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_027994_2_0_1; -.
DR InParanoid; Q9S836; -.
DR OMA; HECKSMC; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q9S836; -.
DR PRO; PR:Q9S836; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9S836; baseline and differential.
DR Genevisible; Q9S836; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010588; P:cotyledon vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0048829; P:root cap development; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Glycoprotein;
KW Membrane; Reference proteome; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..483
FT /note="Auxin transporter-like protein 2"
FT /id="PRO_0000093843"
FT TOPO_DOM 1..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..73
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..226
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..306
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..350
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..374
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..483
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 478
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 483 AA; 54703 MW; 09DC2A45279098FA CRC64;
MENGEKAAET VVVGNYVEME KDGKALDIKS KLSDMFWHGG SAYDAWFSCA SNQVAQVLLT
LPYSFSQLGM LSGILFQLFY GILGSWTAYL ISILYVEYRT RKEREKVNFR NHVIQWFEVL
DGLLGKHWRN VGLAFNCTFL LFGSVIQLIA CASNIYYIND NLDKRTWTYI FGACCATTVF
IPSFHNYRIW SFLGLLMTTY TAWYLTIASI LHGQVEGVKH SGPSKLVLYF TGATNILYTF
GGHAVTVEIM HAMWKPQKFK SIYLFATLYV LTLTLPSASA VYWAFGDLLL NHSNAFALLP
KNLYRDFAVV LMLIHQFITF GFACTPLYFV WEKLIGMHEC RSMCKRAAAR LPVVIPIWFL
AIIFPFFGPI NSTVGSLLVS FTVYIIPALA HIFTFRSSAA RENAVEQPPR FLGRWTGAFT
INAFIVVWVF IVGFGFGGWA SMINFVHQID TFGLFTKCYQ CPPPVMVSPP PISHPHFNHT
HGL
