LAX3_ARATH
ID LAX3_ARATH Reviewed; 470 AA.
AC Q9CA25;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Auxin transporter-like protein 3;
DE AltName: Full=AUX1-like protein 3;
GN Name=LAX3; OrderedLocusNames=At1g77690; ORFNames=T32E8.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND NOMENCLATURE.
RA Parry P.G., Marchant A., May S.T., Swarup R., Swarup K., James N.,
RA Graham N., Allen T., Martucci T., Yemm A., Napier R., Manning K., King G.,
RA Bennett M.J.;
RT "Quick on the uptake: characterization of a family of plant auxin influx
RT carriers.";
RL J. Plant Growth Regul. 20:217-225(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
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DR EMBL; AY127575; AAN02284.1; -; Genomic_DNA.
DR EMBL; AC012193; AAG51630.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36010.1; -; Genomic_DNA.
DR EMBL; AY054266; AAL06925.1; -; mRNA.
DR EMBL; BT008300; AAP37659.1; -; mRNA.
DR PIR; F96806; F96806.
DR RefSeq; NP_177892.1; NM_106418.4.
DR AlphaFoldDB; Q9CA25; -.
DR SMR; Q9CA25; -.
DR BioGRID; 29324; 6.
DR IntAct; Q9CA25; 3.
DR STRING; 3702.AT1G77690.1; -.
DR PaxDb; Q9CA25; -.
DR PRIDE; Q9CA25; -.
DR ProteomicsDB; 238195; -.
DR EnsemblPlants; AT1G77690.1; AT1G77690.1; AT1G77690.
DR GeneID; 844105; -.
DR Gramene; AT1G77690.1; AT1G77690.1; AT1G77690.
DR KEGG; ath:AT1G77690; -.
DR Araport; AT1G77690; -.
DR TAIR; locus:2203226; AT1G77690.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_027994_2_0_1; -.
DR InParanoid; Q9CA25; -.
DR OMA; IRVHETK; -.
DR OrthoDB; 509343at2759; -.
DR PhylomeDB; Q9CA25; -.
DR PRO; PR:Q9CA25; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CA25; baseline and differential.
DR Genevisible; Q9CA25; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0010328; F:auxin influx transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0009926; P:auxin polar transport; IDA:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010311; P:lateral root formation; IGI:TAIR.
DR GO; GO:0009733; P:response to auxin; IDA:TAIR.
DR GO; GO:0048829; P:root cap development; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..470
FT /note="Auxin transporter-like protein 3"
FT /id="PRO_0000093844"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..77
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..170
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..312
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..354
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..426
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..470
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 470 AA; 53305 MW; B6A50B7900DA7130 CRC64;
MAAEKIETVV AGNYLEMERE EENISGNKKS STKTKLSNFF WHGGSVYDAW FSCASNQVAQ
VLLTLPYSFS QLGMMSGILF QLFYGLMGSW TAYLISVLYV EYRTRKEREK FDFRNHVIQW
FEVLDGLLGK HWRNLGLIFN CTFLLFGSVI QLIACASNIY YINDKLDKRT WTYIFGACCA
TTVFIPSFHN YRIWSFLGLA MTTYTSWYLT IASLLHGQAE DVKHSGPTTM VLYFTGATNI
LYTFGGHAVT VEIMHAMWKP QKFKAIYLLA TIYVLTLTLP SASAVYWAFG DKLLTHSNAL
SLLPKTGFRD TAVILMLIHQ FITFGFASTP LYFVWEKLIG VHETKSMFKR AMARLPVVVP
IWFLAIIFPF FGPINSAVGS LLVSFTVYII PALAHMLTFA PAPSRENAVE RPPRVVGGWM
GTYCINIFVV VWVFVVGFGF GGWASMVNFV RQIDTFGLFT KCYQCPPHKP
